Nageh OMRAN, Mostafa HASSAN, Mohamed ABDEL-RAHMAN, Abdel-aleem DESOKY, Stayed HAMOUDA

Mısır Sidr Balının Antimikrobiyal Aktivitesi ve Antimikrobiyal Ajanlarla Sinerjik Etkisi

Bu çalışmanın amacı, Yukarı Mısır'da üretilen Sidr balının beş referans bakteri suşuna (Gram pozitif ve Gram negatif suşlar) karşı in vitro antibakteriyel potansiyel aktivitesini ve bazı antimikrobiyal ajanlarla sinerjistik etkisini belirlemektir. Yukarı Mısır'daki üç valilikten on beş Sidr balı örneği toplanmıştır. Bal örnekleri seyreltilmiş ve agar seyreltme yöntemiyle Staphylococcus aureus, Streptococcus agalactiae, Escherichia coli, Klebsiella pneumoniae ve Bacillus cereus'a karşı test edilmiştir. Minimum inhibitör konsantrasyon (MİK) değerlerinin belirlenmesinden sonra, altı bal örneği etkisiz antimikrobiyal ajanlarla sinerjik etkileri açısından incelenmiştir. In vitro antimikrobiyal duyarlılık testinde, tüm bakteri suşları test edilen 13 antimikrobiyal ajana karşı yüksek çoklu antibiyotik direnci (MAR) indeksi ile çoklu ilaç direnci etkisi göstermiştir, bu değer ≥ 0.38'dir. Tüm Sidr arı balı örnekleri, test edilen beş referans bakteri suşuna karşı antibakteriyel aktivite göstermiştir. Tüm Sidr arı balı örnekleri, tüm antimikrobiyal ajanlara karşı daha iyi sinerjik etki göstermiştir.

Anahtar Kelimeler:

Mısır Sidr balı, Antimikrobiyal etki, Minimum inhibitör konsantrasyon, Sinerjistik etki

ANTIMICROBIAL ACTIVITY OF EGYPTIAN SIDR HONEY AND ITS SYNERGISTIC ACTION WITH ANTIMICROBIAL AGENTS

Determine the in vitro antibacterial potential activity sidr honey produced in upper Egypt against five references bacterial strains (Gram positive and Gram negative strains) and its synergistic effect with some antimicrobial agents. Material & Methods: fifteen Sidr honey samples were collected from three Governorates in Upper Egypt. Honey samples were diluted and tested against Staphylococcus aureus, Streptococcus agalactiae, Escherichia coli, Klebsiella pneumoniae and Bacillus cereus by agar dilution method. Post determination of the minimum inhibitory concentration (MIC) values, six honey samples were examined for their synergistic action with the ineffective antimicrobial agents. Results: In vitro antimicrobial sensitivity test, all bacterial strains showed multidrug resistance action against the 13 tested antimicrobial agents with high multiple antibiotic resistance (MAR) index, it was ≥ 0.38. All Sidr bee honey samples showing antibacterial activity against the five tested references bacterial strains. All Sidr bee honey samples, showed better synergistic effect with all antimicrobial agents against.

Keywords:

Egyptian Sidr honey, Antimicrobial impact, Minimum inhibitory concentration, Synergistic effect,

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