Kemal ÖZTABAK, Feraye ESEN, Aysel ÖZPINAR, Sabiha CİVELEK, Gülden BURÇAK

The effect of energy restricted diet onthe activities of plasma Cu-Zn SOD, GSH-Px, CAT and TBARS concentrations in late pregnant ewes

Enerji kısıtlı diyetle beslemenin gebeliğin son dönemindeki koyunlarda antioksidan savunma sistemi üzerine olan etkilerinin ortaya çıkarılması amacı ile yapılan çalışmamızda 30 adet multiple gebelik oranı yüksek Sakız ırkı koyun kullanılmıştır. Gebe olan koyunlar her bir grupta 10 hayvan olacak şekilde iki gruba ayrılırken (grup II ve III), gebe olmayan 10 adet hayvandan da üçüncü bir grup oluşturulmuştur (grup I). Grup I, II ve IlI'te bulunan koyunlar sırasıyla 9,14 MJ ME/kg kuru madde (KM)-%10,23 Ham protein (HP), 10,20 MJ ME/kg KM-%15,04 HP ve 8,82 MJ ME/kg KM-%14,47 HP içeren rasyonla beslenmişlerdir. Tüm koyunlardan gebeliğin 105. ve 148. günlerinde kan örnekleri toplanmış ve plazma Cu-Zn süperoksid dismutaz (SOD), glutatyon peroksidaz (GSH-Px), katalaz (CAT) aktiviteleri ve tiobarbiturik asit reaktif maddeleri (TBARS) konsantrasyonları tespit edilmiştir. Gebeliğin 105. ve 148. günlerinde plazma Cu-Zn süperoksid dismutaz ve TBARS düzeyleri açısından gruplar arasında farklılık bulunamamıştır. Gebeliğin 148. gününde grup lll'ün katalaz düzeyinin Grup I ve ll'den P < 0,05 düzeyinde daha yüksek olduğu belirlenmiştir. Yine 148. günde Grup lll'ün GSH-Px düzeyinin Grup l'den P < 0,05 düzeyinde daha yüksek olduğu saptanmıştır. Gruplar içinde günler arası yapılan kıyaslamada ise sadece Grup I'in GSH-Px aktivitesinin 148. gündeki artışının 105. günden P < 0,05 düzeyine daha yüksek olduğu belirlenmiştir. Sonuç olarak, gebeliğin sonunda plazma CAT ve GSH-Px konsantrasyonunun yüksek bulunmasının nedeni yavrunun hidrojen peroksidin zararlı etkilerinden korunmasıdır.

Gebeliğin son dönemindeki koyunlarda enerji kısıtlı diyetle beslemenin plazma CuZn SOD, GSH-Px, CAT aktiviteleri ile TBARS konsantrasyonları üzerine etkisi

The aim of the study was to determine the effects of low energy diets on the enzymatic antioxidant activities of ewes in late pregnancy. Thirty Chios ewes were used. Twenty pregnant ewes were divided into 2 groups of 10 ewes each (groups II and III), with 10 non-pregnant ewes being separated into a third group (group I). The ewes in groups I, II and III were fed rations containing 9.14 MJ ME/kg of dry matter (DM)-10.23% crude protein (CP), 10.20 MJ ME/kg of DM-15.04% CP, and 8.82 MJ ME/kg of DM-14.47% CP, respectively. Plasma copper-zinc superoxide dismutase (Cu-Zn SOD), glutathione peroxidase (GSH-Px), catalase (CAT) activities and thiobarbituric acid reactive substances (TBARS) concentrations were measured on days 105 and 148 of pregnancy. No significant differences for Cu-Zn SOD and TBARS were detected among the groups on days 105 and 148 of pregnancy. The catalase activities of group III were higher by P < 0.05 than those of both groups I and II on day 148 of pregnancy. The GSH-Px activities of group III were higher than those of group I on day 148 of pregnancy (P < 0.05). In a 2-day comparison of each group, only GSH-Px activities increased in group III (P < 0.05). It was concluded that the reason for the high plasma CAT and GSH-Px activities at the end of pregnancy is to protect the foetus from the deleterious effects of hydrogen peroxide.

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