Şükrü TONBAK, Aykut ÖZDARENDELİ, Yusuf BOLAT

Cloning of Nucleoprotein Gene (NP) of Rinderpest Virus (RPV) RBOK Vaccine Strain

In the present study cloning of the NP gene in a cloning vector is reported. For this purpose, Vero cells were infected with rinderpest RBOK vaccine strain. Total RNA was obtained from the infected cells and reverse-transcription (RT) was performed to get cDNAs of NP gene. Afterwards, the NP gene was amplified by polymerase chain reaction (PCR) with the primers specific to the NP gene. The NP gene, 1575 bp in length, was cloned into pGEM-3Zf (-) cloning vector. PCR screening and enzyme digestion assays carried out to verify the presence of the NP gene.

Anahtar Kelimeler:

Rinderpest virus, nucleoprotein gene, cloning

Cloning of Nucleoprotein Gene (NP) of Rinderpest Virus (RPV) RBOK Vaccine Strain

Keywords:

Rinderpest virus, nucleoprotein gene, cloning,

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