Avipoxvirus Infection in Quails

The present study describes clinical, virological, histopathological, immunohistochemical and electron microscopic findings of pox infection encountered in a quail flock. Lesions consisted of single or multiple nodules with a crust in variable size of gray to yellow or dark brown discoloration on the comb, eyelids, and the other poorly feathered areas of the body. Histopathological changes consisted of hyperplastic epithelium overlying, with ballooning degeneration of keratinocytes, many of which had eosinophilic intracytoplasmic inclusion bodies (Bollinger bodies), and heterophilic infiltrations admixed with mononuclear cells extending into dermis. Avipoxvirus infection was confirmed by positive immunostaining in the cytoplasm of affected cells for poxvirus antigen, excluded no immunostaining of inclusion bodies, using streptavidin-biotin peroxidase complex method and by demonstration of brick-shaped virus particles with a central core using direct electron microscopy. Typical thickness of chorio-allantoic membranes (CAMs) infected with virus, compared with controls, was observed, and the harvested virus on CAMs was detected as positive against known fowlpox virus using gel diffusion test.

Anahtar Kelimeler:

Avipoxvirus, pox, quail

Avipoxvirus Infection in Quails

Keywords:

Avipoxvirus, pox, quail,

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Bolte, A.L., Meurer, J., Kaleta, E.F.: Avian host spectrum of avipoxviruses. Avian Pathol., 1999; 28: 415- 432. 2. Gough, R.E., Alexander, D.J., Collins, M.S., Lister, S.A.: Routine virus isolation or detection in the diagnosis of diseases in birds. Avian Pathol., 1988; 17: 893-907. 3. Tripathy D.N., Reed, W.M.: Pox. In: Calnek, B.W., Barnes, H.J., Beard, C.W., McDougald, L.R., Saif, Y.M., Eds. Diseases of Poultry. 10th ed., Iowa State University Press, Ames, Iowa, 1997; 643-659. 4.
Moss, B.: Poxviridae and their replication. In: Fields B.N., Knipe,
D.M., Chanock, R.M., Hirsch, M.S., Melnick, J.L., Monath, T.P.,
Roizman, B., Eds. Virology. 2nd ed., Raven Press, New York, 1990; 2079-2111. 5.
Crawford, J.A., Oates, R.M., Helfer, D.H.: Avian pox in California
quail from Oregon. J. Wildlife. Dis., 1979; 15: 447-449. 6.
Davidson, W.R., Kellogg, F.E., Doster, G.L.: An epornitic of avian
pox in wild bobwhite quail. J. Wildlife. Dis., 1980; 16: 293-298. 7. Poonacha, K.B., Wilson, M.: Avian pox in pen-raised bobwhite quail. J. Am. Vet. Med. Assoc., 1981; 179: 1264-1265. 8. Rinaldi, A., Mahnel, H., Nardelli, L., Andelli, G.C., Cervio, G., Valeri, A.: Charakterisierung eines wachtelpockenvirus. Zbl. Vet. Med. B. 1972; 19: 199-212. 9. Gülbahar, M.Y., Çabalar, M., Gül, Y., İçen, H.: Immunohistochemical detection of antigen in lamb tissues naturally infected with sheeppox virus. J. Vet. Med. B., 2000; 46: 173-181.
Singh, S.D., Mohanty, G.C., Kataria, J.M.: Vascular and cellular reactions in quail skin induced by fowl poxvirus. Indian J. Vet. Pathol., 1992; 16: 1-5.
Hernandez, M., Sanchez, C., Galka, M.E., Dominguez, L., Goyache, J., Oria, J., Pizarro, M.: Avian pox infection in Spanish imperial eagles (Aquila adalberti). Avian Pathol. 2001; 30: 91-97.
Loupal, V.G., Schonbauer, M., Jahn, J.: Pocken bei zoo und wildvogeln. Zbl. Vet. Med. B., 1985; 32: 326-336.
Minbay, A., Kreier, J.P.: An experimental study of the pathogenesis of fowlpox infection in chickens. Avian Dis., 1973; 17: 532-539.
Tanizaki, E., Horiuchi, T., Niikura, M., Mikami, T., Wakamiya, N.: Detection of fowlpox virus DNA by in situ hybridization using a biotinylated probe. Avian Dis., 1990; 34: 374-380.
Back, A., Soncini, R.A., Ruthes, O., Madureira, S.Jr., Flores, R.: An atypical fowl pox outbreak in broilers in southern Brazil. Avian Dis., 1995; 39: 902-906.
Tsai, S.S., Chang, T.C., Yang, S.F., Chi, Y.C., Cher, R.S., Chien, M.S., Itakura, C.: Unusual lesions associated with avian poxvirus infection in rosy-faced lovebirds (Agapornis roseicollis). Avian Pathol., 1997; 26: 75- 82.
McDonald, S.E., Lowenstine, L.J., Ardans, A.A.: Avian pox in blue- fronted Amazon parrots. J. Am. Vet. Med. Assoc., 1981; 179: 1218-1222.
Donnelly, T.M., Crane, L.A.: An epornitic of avian pox in a research aviary. Avian Dis., 1984; 28: 517-525.
Eleazer, T.H., Harrel, J.S., Blalock, H.G.: Transmission studies involving a wet fowl pox isolate. Avian Dis., 1983; 27: 542-544.
Ghildyal, N., Schnitzlein, W.M., Tripathy, D.N.: Genetic and antigenic differences between fowlpox and quailpox viruses. Arch. Virol., 1989; 106: 85-92.
Winterfield, R.W., Reed, W.: Avian pox: Infection and immunity with quail, psittacine, fowl, and pigeon pox viruses. Poultry Sci., 1985; 64: 65-70.
Çabalar, M., Voyvoda, H., Sekin, S.: Diagnosis of ecthyma contagiosum (orf) in lambs using direct electron microscopy. Proceeding of 13thNational Electron Microscopy Congress, METU, Ankara. 1997; 131-134.
Tripathy, D.N., Hanson, L.E., Killinger, A.H.: Immunoperoxidase technique for detection of fowlpox antigen. Avian Dis. 1973; 17: 274-278.
Kitamoto, N., Hiroi, K., Miyamoto, K., Tanaka, T., Mityamoto, H.: Virus-specific early antigen expressed in the nucleus of cowpox virus-infected cells. J. Gen. Virol., 1990; 71: 235-240.
Gafford, L.G., Randall, C.C.: Virus-specific RNA and DNA in nuclei of cells infected with fowlpox virus. Virology, 1976; 69: 1-14.
Mockett, A.P.A., Deuter, A., Southee, D.J.: Fowlpox vaccination: routes of inoculation and pathological effects. Avian Pathol., 1990; 19: 613-625.
Moss, B: Poxviridae: the viruses and their replication. In: Fields B.N., Knipe, D.M., Howley, P.M., Chanock, R.M., Melnick, J.L., Monath, T.P., Roizman, B., Eds. Fundamental Virology, 3rd ed. Lippincott-Raven, Philadelphia, 1996; 1163-1197.