Alpaslan GÖKÇİMEN, Yıldırım ERDOĞAN, Hüseyin ASLAN, Tuba ŞENTUT, Zühal KIRZIOĞLU

Quantitative analysis of odontoblast cells in fluorotic and nonfluorotic primary tooth pulp

To determine the effect of fluoride ingested during the development period on the number of odontoblast cells using stereological methods. Materials and methods: A total of 56 freshly extracted primary first and second molar teeth were used, obtained from children (aged between 9 and 11 years) requiring dental extractions for clinical reasons. Following histological procedures, longitudinal tooth sections, 25 µm thick, were stained with hematoxylin and eosin. The total number of odontoblast cells was estimated using the optical fractionator method with a stereological workstation. All of the data were expressed as means ± standard deviations for each group, and statistical analyses were performed using SPSS software. Results: The mean number of odontoblast cells in fluorotic second molar teeth was significantly lower compared to nonfluorotic teeth in the maxillary jaw. Furthermore, a statistically significant difference was found in the mandibular jaw, and the mean number of odontoblast cells in fluorotic second molar teeth was significantly lower than in the nonfluorotic group. Conclusion: Fluoride was found to decrease the number of odontoblast cells in fluorotic teeth, and this finding indicates that fluoride could affect the mechanism of pulpal recovery following operative procedures as a result of fewer odontoblast cells.

Anahtar Kelimeler:

Key words: Dental fluorosis, odontoblast, primary tooth, stereology, tooth pulp

Quantitative analysis of odontoblast cells in fluorotic and nonfluorotic primary tooth pulp

Keywords:

Key words: Dental fluorosis, odontoblast, primary tooth, stereology, tooth pulp,

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Den Besten PK. Dental fl uorosis: its use as a biomarker. Adv Dent Res 1994; 8: 105-10.
Couble ML, Farges JC, Bleicher F, Perrat-Mabillion B, Boudeulle M, Magloire H. Odontoblast diff erentiation of human dental pulp cells in explant cultures. Calcif Tissue Int 2000; 66: 129-38. 3. Murray PE, About I, Lumley PJ, Franquin JC, Remusat M, Smith AJ. Human odontoblast cell numbers aft er dental injury. J Dent 2000; 28: 277-85.
About I, Murray PE, Franquin JC, Remusat M, Smith AJ. Th e eff ect of cavity restoration variables on odontoblast cell numbers and dental repair. J Dent 2001; 29: 109-17. 5. Murray PE, Lumley PJ, Ross HF, Smith AJ. Tooth slice organ culture for cytotoxicity assessment of dental materials. Biomaterials 2000; 21: 1711-21.
Murray PE, About I, Franquin JC, Remusat M, Smith AJ. Restorative pulpal and repair responses. J Am Dent Assoc 2001; 132: 482-91. 7. Murray PE, Smith AJ, Windsor LJ, Mjör IA. Remaining dentine thickness and human pulp responses. Int Endod J 2003; 36: 33- 43. 8. Kahn FH, Rosenberg PA, Schertzer L, Korthals G, Nguyen PN. An in vitro evaluation of sealer placement methods. Int Endod J 1997; 30: 102-14. 9. Sazak S, Günday M, Alatli C. Eff ects of calcium hydroxide and combinations of ledermix and calcium hydroxide on infl amed pulp in dogs’ teeth. J Endod 1996; 22: 447-9.
Kirk EEJ, Meyer MJ. Morphology of the mineralizing front and observations of reparative dentine following induction and inhibition of dentinogenesis in the rat incisor. Endod Dent Traumatol 1992; 8: 195-201.
Geinisman Y, Gundersen HJG, Van Der Zee E, West MJ. Unbiased stereological estimation of the total number of synapses in a brain region. J Neurocytol 1996; 25: 805-19.
Chang YC, Chou MY. Cytotoxicity of fl uoride on human pulp cell cultures in vitro. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2001; 91: 230-4.
Moseley R, Sloan AJ, Waddington RJ, Smith AJ, Hall RC, Embery G. Th e infl uence of fl uoride on the cellular morphology and synthetic activity of the rat dentine-pulp complex in vitro. Arch Oral Biol 2003; 48: 39-46.
Nakade O, Koyama H, Arai J, Ariji H, Takada J, Kaku T. Stimulation by low concentrations of fl uoride of the proliferation and alkaline phosphatase activity of human dental pulp cells in vitro. Arch Oral Biol 1999; 44: 89-92.
Bélanger LF, Visek WJ, Lotz WE, Comar CL. Th e eff ects of fl uoride ingestion on the organic matrix of the teeth of growing pigs. J Dent Res 1958; 37: 264-75.
Th ylstrup A, Fejerskov O. A scanning electron microscopic and microradiographic study of pits in fl uorosed human enamel. Scand J Dent Res 1979; 87: 105-14.
Gundersen HJ. Stereology of arbitrary particles. A review of unbiased number and size estimators and the presentation of some new ones, in memory of William R. Th ompson. J Microsc 1986; 143: 3-45.
Chandler NP, Pitt Ford TR, Monteith BD. Coronal pulp size in molars: a study of bitewing radiographs. Int Endod J 2003; 36: 757-63.
Tziafas D. Basic mechanisms of cytodiff erentiation and dentinogenesis during dental tissue repair. Int J Dev Biol 1995; 39: 281-90.
Murray PE, Stanley HR, Matthews JB, Sloan AJ, Smith AJ. Age- related odontometric changes of human teeth. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2002; 93: 474-82.
Turgut M, Turkkani Tunc A, Aslan H, Yazici AC, Kaplan S. Eff ect of pinealectomy on the morphology of the chick cervical spinal cord: a stereological and histopathological study. Brain Res 2007; 1129: 166-73.
Ünal B, Aslan H, Canan S, Şahin B, Kaplan S. Biyolojik ortamlardaki objelerin sayımı yapılırken kullanılan eski (tarafl ı) metodların önemli hata kaynakları ve çözüm önerileri. T Klin Tıp Bilimleri 2002; 22: 1-6.
West MJ, Gundersen HJG. Unbiased stereological estimation of the number of neurons in the human hippocampus. J Comp Neurol 1990; 296: 1-22.
Eriksson SH, Free SL, Th om M, Martinian L, Sisodiya SM. Methodological aspects of 3D and automated 2D analysis of white matter neuronal density in temporal lobe epilepsy. Neuropathol Appl Neurobiol. 2006; 32: 260-70.
Sahin B, Acer N, Sonmez OF, Emirzeoglu M, Basaloglu H, Uzun A et al. Comparison of four methods for the estimation of intracranial volume: a gold standard study. Clin Anat 2007; 20: 766-73.
Acer N, Sahin B, Baş O, Ertekin T, Usanmaz M. Comparison of three methods for the estimation of total intracranial volume: stereological, planimetric, and anthropometric approaches. Ann Plast Surg 2007; 58: 48-53.
Murray PE, Kitasako Y, Tagami J, Windsor LJ, Smith AJ. Hierarchy of variables correlated to odontoblast-like cell numbers following pulp capping. J Dent 2002; 30: 297-304.
Maurice CG, Schour I. Eff ects of sodium fl uoride upon the pulp of the rat molar. J Dent Res 1956; 35: 69-82.
Brännström M, Nyborg H. Pulp reaction to fl uoride solution applied to deep cavities: an experimental histological study. J Dent Res 1971; 50: 1548-52.