Tarık OCAK, Arif DURAN, Gülzade ÖZYALVAÇLI, Zeynep OCAK

Protective effects of montelukast and Hypericum perforatum against intestinal ischemia-reperfusion injury in hamsters

To evaluate the effects of montelukast and Hypericum perforatum against ischemia/reperfusion (I/R)-induced intestinal damage. Materials and methods: Twenty-eight hamsters were divided into 4 groups following midline abdominal laparotomy: control group (n = 7), I/R group (n = 7), montelukast and I/R (MIR) group (n = 7), and Hypericum perforatum and I/R (HPIR) group (n = 7). After 60 min of ischemia through obstruction of the superior mesenteric artery, 24 h of reperfusion was maintained. Ten minutes prior to the reperfusion period, the MIR group received 7 mg/kg of intraperitoneal montelukast and the HPIR group received 7 mg/kg of intraperitoneal Hypericum perforatum. Malondialdehyde, glutathione, myeloperoxidase, and cardiotrophin-1 levels were measured from blood samples. A semiquantitative histological evaluation was performed. Results: Montelukast and Hypericum perforatum significantly reduced malondialdehyde levels and increased glutathione levels compared to the I/R group (P < 0.008). A statistically significant difference was also found between the I/R group and MIR and HPIR groups in terms of myeloperoxidase levels (P < 0.008). The MIR and HPIR groups showed increased cardiotrophin-1 levels compared to the control and I/R groups (P < 0.008 for all). The MIR and HPIR groups showed significantly lower histological scores compared to the I/R group (P = 0.03 and P = 0.007, respectively). Conclusion: This study demonstrated the preventive effects of montelukast and Hypericum perforatum on I/R-induced intestinal injury.

Anahtar Kelimeler:

Key words: Montelukast, Hypericum perforatum, intestinal ischemia-reperfusion injury

Protective effects of montelukast and Hypericum perforatum against intestinal ischemia-reperfusion injury in hamsters

Keywords:

Key words: Montelukast, Hypericum perforatum, intestinal ischemia-reperfusion injury,

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Kong SE, Blennerhassett LR, Heel KA, McCauley RD, Hall JC. Ischaemia-reperfusion injury to the intestine. Aust N Z J Surg 1998; 68: 554–561.
Cerqueira NF, Hussni CA, Yoshida WB. Pathophysiology of mesenteric ischemia/reperfusion: a review. Acta Cir Bras 2005; 20: 336–343.
Belviranlı M, Okudan N, Gökbel H, Kıyıcı A, Öz M, Kumak A. Cytokeratin 18 and h-FABP levels in intestinal ischemia– reperfusion injury: role of coenzyme Q10. Turk J Med Sci 2013; 43: 6–11.
Li C, Jackson RM. Reactive species mechanisms of cellular hypoxia-reoxygenation injury. Am J Physiol Cell Physiol 2002; 282: 227–241.
Filho DW, Torres MA, Bordin AL, Crezcynski-Pasa TB, Boveris A. Spermatic cord torsion, reactive oxygen and nitrogen species and ischemia-reperfusion injury. Mol Aspects Med 2004; 25: 199–210.
Kelly KJ, Williams WW Jr, Colvin RB, Meehan SM, Springer TA, Gutierrez-Ramos JC, Bonventre JV. Intercellular adhesion molecule-1-deficient mice are protected against ischemic renal injury. J Clin Invest 1996; 97: 1056–1063.
Buege JA, Aust SD. Microsomal lipid peroxidation. Methods Enzymol 1978; 52: 302–310.
Carsin H, Bargues L, Stephanazzi J, Paris A, Aubert P, Le Bever H. Inflammatory reaction and infection in severe burns. Pathol Biol (Paris) 2002; 50: 93–101.
Wallace JL, McKnight GW, Keenan CM, Byles NI, MacNaughton WK. Effects of leukotrienes on susceptibility of the rat stomach to damage and investigation of the mechanism of action. Gastroenterology 1990; 98: 1178–1186.
Sener G, Kabasakal L, Cetinel S, Contuk G, Gedik N, Yegen B. Leukotriene receptor blocker montelukast protects against burn-induced oxidative injury of the skin and remote organs. Burns 2005; 31: 587–596.
Ozturk N, Korkmaz S, Ozturk Y. Wound healing activity of St. John’s wort (Hypericum perforatum L.) on chicken embryonic fibroblasts. J Ethopharmacol 2007; 111: 33–39.
Di Paola R, Mazzon E, Muià C, Crisafulli C, Genovese T, Di Bella P, Esposito E, Menegazzi M, Meli R, Suzuki H et al. Protective effect of Hypericum perforatum in zymosan-induced multiple organ dysfunction syndrome: relationship to its inhibitory effect on nitric oxide production and its peroxynitrite scavenging activity. Nitric Oxide 2007; 16: 118–130.
Paterniti I, Briguglio E, Mazzon E, Galuppo M, Oteri G, Cordasco G, Cuzzocrea S. Effects of Hypericum perforatum, in a rodent model of periodontitis. BMC Complement Altern Med 2010; 10: 73.
Mukherjee PK, Verpoorte R, Suresh B. Evaluation of invivo wound healing activity of Hypericum patulum (family: Hypericaceae) leaf extract on different wound model in rats. J Ethnopharmacol 2000; 70: 315–321.
Samadi S, Khadivzadeh T, Emami A, Moosavi NS, Tafaghodi M, Behnam HR. The effect of Hypericum perforatum on the wound healing and scar of cesarean. J Altern Complement Med 2010; 16: 113–117.
Megison SM, Horton JW, Chao H, Walker PB. A new model for intestinal ischemia in the rat. J Surg Res 1990; 49: 168–173.
Watanabe T, Kobata A, Tanigawa T, Nadatani Y, Yamagami H, Watanabe K, Tominaga K, Fujiwara Y, Takeuchi K, Arakawa T. Activation of the MyD88 signaling pathway inhibits ischemiareperfusion injury in the small intestine. Am J Physiol Gastrointest Liver Physiol 2012; 303: 324–334.
Zhi-Yong S, Dong YL, Wang XH. Bacterial translocation and multiple system organ failure in bowel ischemia and reperfusion. J Trauma 1992; 32: 148–153.
Akman H, Somuncu S, Dikmen G, Ayva Ş, Soyer T, Doğan P, Çakmak M. Protective effect of selenium on intussusceptioninduced ischemia/reperfusion intestinal oxidative injury in rats. Turk J Med Sci 2010; 40: 391–397.
Mondello S, Galuppo M, Mazzon E, Domenico I, Mondello P, Carmela A, Cuzzocrea S. Glutamine treatment attenuates the development of ischaemia/reperfusion injury of the gut. Eur J Pharmacol 2010; 643: 304–315.
Sun Q, Meng QT, Jiang Y, Xia ZY. Ginsenoside Rb1 attenuates intestinal ischemia reperfusion induced renal injury by activating Nrf2/ARE pathway. Molecules 2012; 17: 7195–7205.
Hazinedaroglu SM, Dulger F, Kayaoglu HA, Pehlivan M, Serinsoz E, Canbolat O, Erverdi N. N-acetylcysteine in intestinal reperfusion injury: an experimental study in rats. Aust N Z J Surg 2004; 74: 676–678.
Sizlan A, Guven A, Uysal B, Yanarates O, Atim A, Oztas E, Cosar A, Korkmaz A. Proanthocyanidin protects intestine and remote organs against mesenteric ischemia/reperfusion injury. World J Surg 2009; 33: 1384–1391.
Vasileiou I, Kalimeris K, Nomikos T, Xanthopoulou MN, Perrea D, Agrogiannis G, Nakos G, Kostopanagiotou G. Propofol prevents lung injury following intestinal ischemiareperfusion. J Surg Res 2012; 172: 146–152.
Önder A, Kapan M, Gümüş M, Yüksel H, Böyük A, Alp H, Başarılı MK, Firat U. The protective effects of curcumin on intestine and remote organs against mesenteric ischemia/ reperfusion injury. Turk J Gastroenterol 2012; 23: 141–147.
Li C, Jackson RM. Reactive species mechanisms of cellular hypoxia-reoxygenation injury. Am J Physiol Cell Physiol 2002; 282: 227–241.
Aydın M, Çelik S. Effects of lycopene on plasma glucose, insulin levels, oxidative stress, and body weights of streptozotocininduced diabetic rats. Turk J Med Sci 2012; 42: 1406–1413.
Wang GZ, Yao JH, Jing HR, Zhang F, Lin MS, Shi L, Wu H, Gao DY, Liu KX, Tian XF. Suppression of the p66shc adapter protein by protocatechuic acid prevents the development of lung injury induced by intestinal ischemia reperfusion in mice. J Trauma Acute Care Surg 2012; 73: 1130–1137.
Watson MJ, Ke B, Shen XD, Gao F, Busuttil RW, KupiecWeglinski JW, Farmer DG. Treatment with antithymocyte globulin ameliorates intestinal ischemia and reperfusion injury in mice. Surgery 2012; 152: 843–850.
Nosáľová V, Drábiková K, Sotníková R, Nosáľ R. Enhanced chemiluminescence of rat small intestine after mesenteric ischaemia/reperfusion. Biologia: Section Cellular and Molecular Biology 2005; 60: 141–143.
Lojek A, Číž M, Slavíková H, Dušková M, Vondráček J, Kubala L, Rácz I, Hamar J. Leukocyte mobilization, chemiluminescence response, and antioxidative capacity of the blood in intestinal ischemia and reperfusion. Free Rad Res 1997; 27: 359–367.
Aguilar-Melero P, Luque A, Machuca MM, Perez de Obanos MP, Navarrete R, Rodríguez-García IC, Briceno J, Iniguez M, Ruiz J, Prieto J et al. Cardiotrophin-1 reduces ischemia/ reperfusion injury during liver transplant. J Surg Res 2013; 181: e83–e91.