Füsun CAN, Arıkan Özay AKAN, İstar DOPALÇI, Esra KOYUNCU, Zeynep Ceren KARAHAN, Alper TEKELİ

Genotyping of Trichosporon asahii strains isolated from urinary tract infections in a Turkish university hospital

Trichosporon infections are associated with a wide spectrum of clinical manifestations. Trichosporon asahii is the most important species in the genus. Our aim was to evaluate the genotypic relatedness of urinary T. asahii strains isolated from immunocompetent patients during a hospital outbreak. Materials and methods: Twenty-three T. asahii isolates obtained from 15 patients hospitalized in different wards of Ankara University Ibn-i Sina Hospital between July 2004 and April 2005 were studied. T. asahii was identified by API 20C AUX (BioMérieux Vitek, Hazelwood, USA) system and confirmed by genus and species specific polymerase chain reactions. Genetic relatedness of the isolates was evaluated by randomly amplified polymorphic DNA (RAPD) and repetitive element PCR (REP-PCR) analyses. Results: RAPD analysis divided the isolates into 5 groups. The results of REP-PCR correlated well with the data obtained by RAPD analysis, although this method produced 3 different groups. Conclusion: In this study of urinary trichosporonosis, 19 of the 23 isolates were found to be genotypically related, suggesting that a single genotype was prevalent in our hospital environment. The genotypically unrelated 4 isolates were probably of endogenous origin. RAPD and REP-PCR analyses can be used for genotyping T. asahii strains.

Anahtar Kelimeler:

Key words: Trichosporon asahii, genotyping

Genotyping of Trichosporon asahii strains isolated from urinary tract infections in a Turkish university hospital

Keywords:

Key words: Trichosporon asahii, genotyping,

PDF

___

Wolf DG, Falk R, Hacham M, Theelen B, Boekhout T, Scorzetti
G et al. Multidrug-resistant Trichosporon asahii infection of
nongranulocytopenic patients in three intensive care units. J
Clin Microbiol 2001; 39: 4420-5.
Mori Y, Hiraoka M, Katsu M, Tsukahara H, Mikami Y, Mayumi M. Marked renal damage in a child with hydronephrosis infected by Trichosporon asahii. Pediatr Nephrol 2005; 20: 234- 6.
Ghiasian SA, Maghsood AH, Mirhendi SH. Disseminated, fatal Trichosporon asahii infection in a bone marrow transplant recipient. J Microbiol Immunol Infect 2006; 39: 426-429.
Rodriguez-Tudela JL, Diaz-Guerra TM, Mellado E, Cano V, Tapia C, Perkins A et al. Susceptibility patterns and molecular identification of Trichosporon species. Antimicrob Agents Chemother 2005; 49: 4026-34.
Lo Passo C, Pernice I, Celeste A, Perdichizzi G, Todaro-Luck F. Transmission of Trichosporon asahii oesophagitis by a contaminated endoscope. Mycoses 2001, 44: 13-21.
Richardson MD, Warnock DW. Uncommon yeast infections. In: Richardson MD and Warnock DW, Fungal Infection Diagnosis and Management. USA: Blackwell Science Ltd., p. 232-234, 1997.
Schleupner CJ, Hamilton JR. A pseudoepidemic of pulmonary fungal infections related to fiberoptic bronchoscopy. Infect Control 1980; 1: 38-42.
Stone J, Manasse R. Pseudoepidemic of urinary tract infections due to Trichosporon beigelii. Infect Control Hosp Epidemiol 1989; 10: 312-5.
Ağırbaşlı H, Bilgen H, Keceli Özcan S, Otlu B, Sinik G, Çerikçioğlu N et al. Two possible cases of Trichosporon infections in bone-marrow-transplanted children: the first case of T. japonicum isolated from clinical specimens. Jpn J Infect Dis 2008; 61: 130-132.
Bayramoglu G, Sonmez M, Tosun I, Aydin K, Aydin F. Breakthrough Trichosporon asahii fungemia in neutropenic patient with acute leukemia while receiving caspofungin. Infection 2008; 36: 68-70.
Karabay O, Madariaga MG, Kocoglu E, İnce N, Kandirali E. Trichosporon asahii fungemia in a patient with non- hematological malignancy. Jpn J Infect Dis 2006; 59: 129-131.
Yıldıran A, Kücüködük Ş, Saniç A, Belet N, Güvenli A. Disseminated Trichosporon asahii infection in a preterm. Am J Perinatol 2003; 20: 269-271.
Sugita T, Ichikawa T, Matsukura M, Sueda M, Takashima M, Ikeda R et al. Genetic diversity and biochemical characteristics of Trichosporon asahii isolated from clinical specimens, houses of patients with summer-type-hypersensitivity pneumonitis and environmental materials. J Clin Microbiol 2001; 39: 2405-11.
Carnovale S, Lorenzo J, Kaufman S, Finquelievich J, Guelfand L. Genotypic study of strains belonging to the genus Trichosporon. Medical Mycology 2007; 45: 51-6.
Sugita T, Nishikawa A, Shinoda T. Identification of Trichosporon asahii by PCR based on sequences of the internal transcribed spacer regions. J Clin Microbiol 1998; 36: 2742-4.
Arikan S, Hasçelik G. Comparison of NCCLS microdilution method and Etest in antifungal susceptibility testing of clinical Trichosporon asahii isolates. Diagn Microbiol Infect Dis 2002; 43: 107-111.
Albufera U, Bhugaloo-Vial P, Issack MI, Jaufeerally-Fakim Y. Molecular characterization of Salmonella isolates by REP-PCR and RAPD analysis. Infect Genet Evol 2009; 9: 322-327.
Rasschaert G, Houf K, Imberechts H, Grijspeerdt K, De Zutter L, Heyndricz M. Comparison of five repetitive-sequence-based PCR typing methods for molecular discrimination of Salmonella enterica isolates. J Clin Microbiol 2005; 43: 3615- 3623.