Müslüm KUZU, Mehmet ÇİFTCİ

3438

Purification and characterization of NADPH-cytochrome P450 reductase from Lake Van fish liver microsomes and investigation of some chemical and metals' effects on the enzyme activity

Purification and characterization of NADPH-cytochrome P450 reductase from Lake Van fish liver microsomes and investigation of some chemical and metals' effects on the enzyme activity

Abstract: NADPH-cytochrome P450 reductase was purified from Lake Van fish liver microsomes by primary and secondary DEAE-cellulose column chromatograph with 20.46 mu M/min/mg enzyme specific activities, 54.4% purification yield, and purification of 38-fold. The purity of the enzyme was established, and its monomer molecular weight was determined by SDS-polyacrylamide gel electrophoresis. SDS-PAGE results showed a single band and the molecular weight of NADPH-cytochrome P450 reductase was 70 kDa. In addition, optimum ionic strength, optimum pH, optimum temperature, and stable pH values were determined for the enzyme in the kinetic studies performed. K_M and V_{max} were determined for NADPH and cytochrome c. Effects of some metals ions, antibiotics, and some other drugs used in aquarium fisheries on the activity of the enzyme were investigated. IC50 values and K_i values of metals showing an inhibitory effect were calculated.
PDF

___

  • Zangar, R. C.; Davydov, D. R. Verma, S. Toxicol. Appl. Pharm. 2004, 199, 316– 331.
  • Lu, A. Y. H.; Junk, K. W.; Coon, M. J. J. Biol. Chem. 1969, 244, 3714–3721.
  • Arın ̧c, E.; Philpot, R. M. J. Biol. Chem. 1976, 251, 3213–3220.
  • Vogel, F.; Lumper, L. Biochem. J. 1986, 236, 871–878.
  • Zhang, H.; Gruenke, L.; Saribas, A. S.; Im, S.; Shen, A. L.; Kasper, C. B.; Waskell, L. Biochemistry-US. 2003, 42, 6804–6813.
  • Laursen, T.; Jensen, K.; Møller, B. L. Biochim. Biophys. Acta. 2010, 1814, 132–138.
  • Arin ̧c, E.; Çelik, H. J. Biochem. Mol. Toxic. 2002, 16, 286–297.
  • Morant, M.; Bak, S.; Moller, B. L.; Werck-Reichhart, D. Curr. Opin. Biotech. 2003, 14, 151–162.
  • McMillan, K.; Bredt, D. S.; Hirsch, D. J.; Snyder, S. H.; Clark, J. E.; Masters, B. S. S. P. Natl. Acad. Sci. USA 1992, 89, 11141–11145.
  • Leclerc, D.; Wilson, A.; Dumas, R.; Gafuik, C.; Song, D.; Watkins, D.; Heng, H. H. Q.; Rommens, J. M.; Scherer, S. W.; Rosenblatt, D. S.; et al. P. Natl. Acad. Sci. USA 1998, 95, 3059–3064.
  • Paine, M. J. I.; Garner, A. P.; Powell, D.; Sibbald, J.; Sales, M.; Pratt, N.; Smith, T.; Tew, D. G.; Wolf, C. R. J. Biol. Chem. 2000, 275, 1471–1478.
  • Hass, E.; Horecker, B. L.; Hogness, T. T. J. Biol. Chem. 1940, 136, 747–774.
  • Horecker, B. L. J. Biol. Chem. 1950, 183, 593–605.
  • Kobayashi, S.; Rikans, L. E. Comp. Biochem. Phys. B 1984, 77, 313–318.
  • Yasukochi, Y.; Okita, R. T.; Masters, B. S. S. Arch. Biochem. Biophys. 1980, 202, 491–498.
  • Haniu, M.; Iyanagi, T.; Miller, P.; Lee, T. D.; Shively, J. E. Biochemistry-US 1986, 25, 7906–7911.
  • Iscan, M. Y.; Arin ̧c, E. Int. J. Biochem. 1986, 18, 731–741.
  • Iscan, M. Y.; Arin ̧c, E. Int. J. Biochem. 1988, 20, 1189–1196.
  • Guengerich, F. P.; Wang, P.; Mason, P. S. Biochemistry-US 1981, 20, 2379–2385.
  • Muto, N.; Tan, L. Biochem. Cell Biol. 1986, 64, 184–193.
  • Nisimoto, Y.; Otsuka-Murakami, H.; Iwata, S. Biochem. J. 1994, 297, 585–593.
  • Ravindranath, V.; Anandatheerthavarada, H. K.; Shankar, S. K. Biochem. Pharmacol. 1990, 39, 1013–1018.
  • Shephard, E. A.; Pike, S. F.; Rabin, B. R.; Phillips, I. R. Anal. Biochem. 1983, 129, 430–433.
  • Porter, T. D.; Kasper, C. B. P. Natl. Acad. Sci. USA 1985, 82, 973–977.
  • Bergh, A. F.; Strobel, H. W. J. Neurochem. 1992, 59, 575–581.
  • Klotz, A. V.; Stegeman, J. J.; Walsh, C. Arch. Biochem. Biophys. 1983, 226, 578–592.
  • Williams, D. E.; Becker, R. R.; Potter, D. W.; Guengerich, F. P.; Buhler, D. R. Arch. Biochem. Biophys. 1983, 225, 55–66.
  • Sen, A.; Arinc, E. J. Biochem. Mol. Toxic. 1997, 12, 103–113.
  • Bozcaarmutlu, A. J. Biochem. Mol. Toxic. 2007, 21, 340–347.
  • Comakli, V.; Akkemik, E.; Ciftci, M.; Kufrevioglu, O. I. Toxicol. Ind. Health. Epub ahead of print 30 January 2013. doi: 10.1177/0748233713475514.
  • Bozcaarmutlu, A.; Arin ̧c, E. Toxicol. in Vitro 2007, 21, 408–416.
  • Hashemi, S.; Nasrollah, A.; Rajabi, M. EXCLI Journal 2013, 12, 384–395.
  • Manyanga, V.; Dhulipalla, R. L.; Hoogmartens, J.; Adams, E. J. Chromatogr. A 2010, 1217, 3748–3753.
  • Usluer, G. ANKEM Derg. 2007, 21, 45–51.
  • Aoi, K.; Fujii-Kuriyama, Y.; Tashiro, Y. J. Cell Sci. 1981, 50, 181–198.
  • Kappus, H. Biochem. Pharmacol. 1986, 35, 1–6.
  • Sinha, B. K. Chem.-Biol. Interact. 1989, 69, 293–317.
  • Portal, P.; Villamil, S. F.; Alonso, G. D.; De Vas, M. G.; Flawia, M. M.; Torres, H. N.; Paveto, C. Mol. Biochem. Parasit. 2008, 160, 42–51.
  • Sari, M. Environ. Biol. Fish. 2008, 81, 21–23.
  • Wang, P.; Mason, P. S.; Guengerich, F. P. Arch. Biochem. Biophys. 1981, 99, 206–219.
  • Laporte, F.; Doussiere, J.; Mechin, V.; Vignais, P. V. Eur. J. Biochem. 1991, 196, 59–66.
  • Buege, A. J.; Aust, S. D. BBA-Gen. Subjects 1975, 385, 371–379.
  • Voznesensky, A. I.; Schenkman, J. B. Eur. J. Biochem. 1992, 210, 741–746.
  • Mori, T.; Yamazaki, A.; Kinoshita, T.; Imaoka, S. Life Sci. 2006, 79, 247–251.
  • Şen, A. PhD, Graduate School of Natural and Applied Science, Middle East Technical University, Turkey, 1997.
  • Pillai, V. C.; Mehvar, R. J. Pharm. Sci. 2011, 100, 3495–3505.
  • Kim, J. S.; Ahn, T.; Yim, S. K.; Yun, C. H. Biochemistry-US 2002, 41, 9438–9447.
  • Bozcaarmutlu, A.; Celik, H.; Arin ̧c, E. Toxicol. Lett. 2011, 205, 192.
  • Levesque, H. M.; Moon, T. W.; Campbell, P. G. C.; Hontela, A. Aquat. Toxicol. 2002, 60, 257–267.
  • Masters, B. S. S.; Williams, C. H.; Kamin, H. Method. Enzymol. 1967, 10, 565–573.
  • Yonetani, T. J. Biol. Chem. 1965, 240, 4509–4514.
  • Lowry, O. H.; Rosebrough, N. J.; Farr, A. L.; Randall, R. J. J. Biol. Chem. 1951, 193, 265–275.
  • Laemmli, U. K. Nature 1967, 227, 680–685.
  • Lineweaver, H.; Burk, D. J. Am. Chem. Soc. 1934, 56, 658–666.
Turkish Journal of Chemistry-Cover
  • ISSN: 1300-0527
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
Arşiv
Sayıdaki Diğer Makaleler

Synthesis, properties, and electrochemistry of a photochromic compound based on dithienylethene and ProDOT

Melek PAMUK ALGI, Atilla CİHANER, Fatih ALGI

Secondary metabolites from Sambucus ebulus

İrem ATAY, Hasan KIRMIZIBEKMEZ, Ahmet Ceyhan GÖREN, Erdem YEŞİLADA

Preparation of visible light sensitive nano-sized N-TiO_2 photocatalysts and their photocatalytic activity under visible light

Young-Gu KANG, Kwang-Hyeok LEE, Hyun-Sik HAHM

Synthesis and properties of novel magnetic nanoparticles grafted with nitropyridine-substituted calix[4]arene derivative as Cr^{6+} extractant

Serkan SAYIN, Vildan DOĞAN

Deposition of cadmium sulfide and zinc sulfide thin films by aerosol-assisted chemical vapors from molecular precursors

Ashfaque Ahmed MEMON, Malik DILSHAD, Neerish REVAPRASADU

Synthesis of methyl 4-(9H-carbazole-9-carbanothioylthio) benzoate: electropolymerization and impedimetric study

Murat ATEŞ, Nesimi ULUDAĞ, Fatih ARICAN, Tolga KARAZEHİR

Synthesis and characterization of substituted benzimidazole Co(II), Fe(II), and Zn(II) complexes and structural characterization of dichlorobis{1-[2-(1-piperidinyl)ethyl]-1H-benzimidazole-_KN^3} zinc(II)

Hasan KÜÇÜKBAY, Ülkü YILMAZ, Orhan BÜYÜKGÜNGÖR, Mehmet AKKURT

Electrochemical monitoring of the interaction between mitomycin C and DNA at chitosan--carbon nanotube composite modified electrodes

Pembe Ece CANAVAR, Ece EKŞİN, Arzum ERDEM

Synthesis and properties of novel magnetic nanoparticles grafted with nitropyridine-substituted calix[4]arene derivative as Cr6+ extractant

Serkan SAYIN, Vildan DOĞAN

Synthesis and molecular modeling studies of naproxen-based acyl hydrazone derivatives

Tuğba TAŞKIN TOK, Özgün ÖZAŞIK, Deniz SARIGÖL, Ayşe UZGÖREN BARAN