Momen ZAREH, Nashwa FARGHALY, Ahmed FARIED

Micromorphological studies on the genus Lotus L. (Fabaceae: Loteae) from Egypt

Trichomes, epidermal cell features, and seed coat sculpturing of 12 Lotus taxa from Egypt were studied using scanning electron microscopy. In addition, statistical analysis for the taxa under investigation was carried out. The results revealed that trichomes have high taxonomic significances between studied taxa. Anticlinal and periclinal cell walls and epidermal cell shape for the stem, leaf, and calyx for each taxon were examined. Seed coat sculpturing exhibited 4 main different surface patterns categories: rugose, reticulate, sulcate, and verrucate. These features can serve as good diagnostic characters at the species level of the genus Lotus.

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Abou-El-Enain MM, Loutfy MH (1999). SEM of seed coat surface characters and the taxonomic relationships in the genus Sesbania Scop. (Leguminosae-Papilionoideae). Taeckholmia 19: 53-61.
Abou-El-Enain M, Loufty M, Shehata A (2007). Seed surface characters and their systematic significance in the genus Lathyrus (Leguminosae, Papilionoideae, Vicieae). Feddes Repertorium 118: 269-285.
Albert S, Sharma B (2013). Comparative foliar micromorphological studies of some Bauhinia (Leguminosae) species. Turk J Bot 37: 276-281.
Arambarri AM (1999). Illustrated catalogue of Lotus L. seeds (Fabaceae). In: Beuselinck PR, editor. Trefoil: The Science and Technology of Lotus. Madison, WI, USA: Crop Science Society of America and American Society of Agronomy, pp. 21-41.
Ávalos SV, Salinas AD (2003). Los tricomas foliares en la caracterización de un grupo de especies del género Quercus, sección Lobatae (Fagaceae). Anales del Instituto de Biología, Universidad Nacional Autónoma de México, Serie Botánica 74: 5-15 (in Spanish).
Barthlott W (1981). Epidermal and seed surface characters of plants: systematic applicability and some evolutionary aspects. Nord J Bot 1: 345-355.
Barthlott W (1990). Scanning electron microscopy of the epidermal surface in plants. In: Claugher D, editor. Scanning Electron Microscopy in Taxonomy and Functional Morphology. Oxford, UK: Clarendon Press, pp. 69-94.
Boesewinkel FD, Bouman F (1984). The seed: structure. In: Johri B, editor. Embryology of Angiosperms. Berlin, Germany: Springer, pp. 567-610.
Boulos L (2009). Flora of Egypt. Checklist. Cairo, Egypt: Al Hadara Publishing.
Brand A (1898). Monographie der Gattung Lotus. Botanische Jahrbücher fur Systematik 25: 166-232 (in German).
Chernoff M, Plitmann U, Kislev M (1992). Seed characters and testa texture in species of the Vicieae: their taxonomic significance. Israel J Bot 41: 167-186.
Christenhusz MJM, Byng JW (2016). The number of known plants species in the world and its annual increase. Phytotaxa 261: 201-217.
Çildir H, Kahraman A, Dogan M (2012). Petal and sepal epidermal micromorphology of six Lathyrus taxa (Fabaceae) and their systematic value. Not Bot Horti Agrobo 40: 35-41.
Dadandı MY, Yıldız K (2015). Seed morphology of some Silene L. (Caryophyllaceae) species collected from Turkey. Turk J Bot 39: 280-297.
Degtjareva G, Kramina T, Sokoloff D, Samigullin T, ValiejoRoman C, Antonov A (2006). Phylogeny of the genus Lotus (Leguminosae, Loteae): evidence from nrITS sequences and morphology. Botany 84: 813-830.
Erkul S, Celep F, Aytaç Z (2015). Seed morphology and its systematic implications for genus Oxytropis DC. (Fabaceae). Plant Biosyst 149: 875-883.
Escaray FJ, Menendez AB, Gárriz A, Pieckenstain FL, Estrella MJ, Castagno LN, Carrasco P, Sanjuán J, Ruiz OA (2012).
Ecological and agronomic importance of the plant genus Lotus. Its application in grassland sustainability and the amelioration of constrained and contaminated soils. Plant Sci 182: 121-133.
Fawzi NM (2011). Macro- and micromorphological seed characteristics of some selected species of CaesalpinioicleaeLeguminosae. Research Journal of Botany 6: 68-77.
Fayed AA, Zareh M, Hassan N, Faried A (2015). A systematic revision of the genus Teucrium (Lamiaceae) in Egypt. Nord J Bot 33: 389-400.
Gillett J (1958). Lotus in Africa south of the Sahara (excluding the Cape Verde islands and Socotra) and its distinction from Dorycnium. Kew Bulletin 13: 361-381.
Heyn C (1970). Studies in Lotus. 3. The L. angustissimus group. Israel J Bot 19: 271-292.
Heyn CC (1967). The Lotus creticus group. Kew Bulletin 21: 299-309.
Heywood VH (1971). Scanning Electron Microscopy. Systematic and Evolutionary Applications. London, UK: Academic Press.
Kramina T, Sokoloff D (2004). A taxonomic study of Lotus australis complex (Leguminosae), with special emphasis on plants from Pacific Ocean islands. Adansonia 26: 171-197.
Kramina TE (1999). A contribution to the taxonomic revision of the Lotus corniculatus complex (Leguminosae, Loteae) in the European part of the former USSR. Systematics and Geography of Plants 68: 265-279.
Kramina TE (2006). A contribution to the taxonomic revision of the Lotus angustissimus-complex (Leguminosae, Loteae). Wulfenia 13: 57-92.
Lersten NR, Curtis JD (1996). Survey of leaf anatomy, especially secretory structures, of tribe Caesalpinieae (Leguminosae, Caesalpinioideae). Plant Syst Evol 200: 21-39.
Lewis GP, Schrire B, Mackinder B, Lock M (2005). Legumes of the World. Kew, UK: Royal Botanic Gardens.
Metcalfe CR, Chalk L (1950). Leguminosae. In: Metcalfe CR, Chalk K, editors. Anatomy of the Dicotyledons. 1st ed. Oxford, UK: Clarendon Press, pp. 476-501.
Mirzaei L, Assadi M, Nejadsatari T, Mehregan I (2015). Seed surface morphology in some species of the Colutea L. (Fabaceae) in Iran. Advances in Bioresearch 6: 105-109.
Murthy G, Sanjappa M (2002). SEM studies on seed morphology of Indigofera L. (Fabaceae) and its taxonomic utility. Rheedea 12: 21-51.
Ojeda I, Francisco-Ortega J, Cronk QC (2009). Evolution of petal epidermal micromorphology in Leguminosae and its use as a marker of petal identity. Ann Bot-London 104: 1099-1110.
Ponomarenko S, Khrzhanovskiĭ YV, Buivids K (1990). Seed structure of species in the genus Cassia (Tourn) L. Adaptatsionnaya izmenchivostrasteniĭ pri introduktsii 40: 162-164 (in Russian with abstract in English).
Roe KE (1971). Terminology of hairs in the genus Solanum. Taxon 20: 501-508.
Rohlf F (2005). NTSYS-pc: Numerical Taxonomy and Multivariate Analysis System, Version 2.2. Setauket, NY, USA: Exeter Publishing Ltd.
Saheed SA, Illoh HC (2010). A taxonomic study of some species in Cassiinae (Leguminosae) using leaf epidermal characters. Not Bot Horti Agrobo 38: 21-27.
Sandral G, Remizowa MV, Sokoloff DD (2006). A taxonomic survey of Lotus section Pedrosia (Leguminosae, Loteae). Wulfenia 13: 97-192.
Stearn WT (1966). Botanical Latin. 2nd ed. London, UK: Nelson.
Stearn WT (2009). Botanical Latin. 4th ed. London, UK: Nelson.
Stenglein SA, Colares MN, Arambarri AM, Novoa MC, Vizcaíno CE, Katinas L (2003). Leaf epidermal microcharacters of the Old World species of Lotus (Leguminosae: Loteae) and their systematic significance. Aust J Bot 51: 459-469.
Uphof JCT (1962). Plant hairs. In: Hummel K, editor. Encyclopedia of Plant Anatomy. 2nd ed. Berlin, Germany: Gebrüder Borntraeger, pp. 1-206.
Vural C, Ekici M, Akan H, Aytaç Z (2008). Seed morphology and its systematic implications for genus Astragalus L. sections Onobrychoidei DC., Uliginosi Gray and Ornithopodium Bunge (Fabaceae). Plant Syst Evol 274: 255-263.
Yagueddú C, Comparatore V, Paoletti G (2009). Identification of six Papilionaceae species by epidermal characteristics: microanalysis of handcomposed mixtures. Boletín de la Sociedad Argentina de Botánica 44: 305-315.
Yildiz K (2002). Seed morphology of Caryophyllaceae species from Turkey (north Anatolia). Pak J Bot 34: 161-171.
Zareh M (2005). SDS-PAGE seed proteins and seed coat characters of Hippocrepis L. (Leguminosae) in Egypt. Assiut University Journal of Botany 34: 1-12.
Zorić L, Merkulov L, Luković J, Boža P (2010). Comparative seed morphology of Trifolium L. species (Fabaceae). Period Biol 112: 263-272.
Zoric L, Merkulov L, Lukovic J, Boza P, Polic D (2009). Leaf epidermal characteristics of Trifolium L. species from Serbia and Montenegro. Flora 204: 198-209.