SAPAN KUMAR SHARMA, NANDINI GAUTAM

Metacordyceps dhauladharensis sp. nov., a new entomopathogenic fungus from India

This paper describes a new species of Clavicipitaceae fungi belonging to the genus Metacordyceps G.H. Sung, J.M. Sung, Hywel-Jones and Spatafora, defined as Metacordyceps dhauladharensis sp. Nov., from India. The diagnostic features of this new species are stipitate stromata of brownish color and an elongated fertile head on a Hymenopteran host. The perithecium size of this species was measured as 152-257 Ã 65-72 µm, and the asci were measured as 30-48 µm in length and 2.5 µm in breadth. Based on molecular studies, the sequence of 469 bp of this species showed only 86% homology with Metacordyceps indigotica (Kobayasi and Shimizu) Kepler, G.H. Sung and Spatafora.

Anahtar Kelimeler:

Entomopathogenic fungi, Metacordyceps, ITS1-5.8S-ITS2, new species

Metacordyceps dhauladharensis sp. nov., a new entomopathogenic fungus from India

Keywords:

Entomopathogenic fungi, Metacordyceps, ITS1-5.8S-ITS2, new species,

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Chang ST, Quimio TH (1982). Tropical Mushrooms: Biological Nature and Cultivation Methods. 1st ed. Hong Kong: Chinese University Press.
Hibbett DS, Binder M, Bischoff JF, Blackwell M, Cannon PF, Eriksson OE, Huhndorf S, James T, Kirk PM, Lücking R et al. (2007). A higher-level phylogenetic classification of the Fungi. Mycol Res 111: 509–547.
Hywel-Jones NL (2002). Multiples of eight in Cordyceps ascospores. Mycol Res 106: 1–3.
Kepler R, Sung GH, Ban S, Nakagiri A, Chen MJ, Huang B, Li Z, Spatafora JW (2012). New teleomorph combinations in the entomopathogenic genus Metacordyceps. Mycologia 104: 182– 197.
Kobayasi Y (1982). Keys to the taxa of the genera Cordyceps and Torrubiella. T Mycol Soc Jpn 23: 329–364.
Kornerup A, Wanscher JH (1978). Methuen Handbook of Colour. 3rd ed. London, UK: Eyre Methuen.
Liu ZY, Yao YJ, Liang ZQ, Liu AY, Pegler DN, Chase MW (2001). Molecular evidence for the anamorph–teleomorph connection in Cordyceps sinensis. Mycol Res 105: 827–832.
Mains EB (1958). North American entomogenous species of Cordyceps. Mycologia 50: 169–222.
Roberts DW (1989). World picture of biological control of insects by fungi. Mem I Os Cr 84: 89–100.
Rossman AY, Samuels GJ, Rogerson CT, Lowen R (1999). Genera of Bionectriaceae, Hypocreaceae and Nectriaceae (Hypocreales, Ascomycetes). Stud Mycol 42: 1–248.
Stensrud Ø, Hywel–Jones NL, Schumacher T (2005). Towards a phylogenetic classification of Cordyceps: ITS nrDNA sequence data confirm divergent lineages and paraphyly. Mycol Res 109: 41–56.
Sung GH, Hywel-Jones NL, Sung JM, Luangsa-ard JJ, Shrestha B, Spatafora JW (2007). Phylogenetic classification of Cordyceps and the clavicipitaceous fungi. Stud Mycol 57: 5–59.
Sung GH, Shrestha B, Sung JM (2010). Characteristics of Metacordyceps yongmunensis, a new species from Korea. Mycobiology 38: 171–175.
Tamura K, Dudley J, Nei M, Kumar S (2007). MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24: 1596–1599.
Uchiyama S, Udagawa S (2002). Cordyceps owariensis f. viridescens and its new Nomuraea anamorph. Mycoscience 43: 135–141.
White TJ, Bruns TD, Lee S, Taylor J (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfend DH, Sninsky JJ, editors. PCR Protocols: a Guide to Methods and Applications. 1st ed. San Diego, CA, USA: Academic Press, pp. 315–322.