Comparative micromorphological and phytochemical studies on the roots of three Viburnum (Caprifoliaceae) species
In this study, the roots of Viburnum punctatum Buch.-Ham. ex D.Don, Viburnum coriaceum Blume, and Viburnum erubescens Wall. ex DC. were collected from the Nilgiri and Coimbatore hills in Tamil Nadu, India. Transverse sections of plant roots were prepared with the aid of a rotary microtome. The sections, each at a thickness of 10 µm, were stained and fixed in Canada balsam and the morphoanatomical features of each specimen were noted. The specimens were powdered using a mechanical grinder and were mounted in suitable media for starch grains, sclereids, druses, fibres, and vessels. Morphoanatomical features of diagnostic importance were recorded under different magnifications, followed by the determination of dimension and histological features of root components using a calibrated eyepiece micrometer and a polariser. The starch grains averaged 12, 17, and 20 µm in diameter in V. erubescens, V. punctatum, and V. coriaceum, respectively. The fibres were about 1 mm long in V. punctatum and V. coriaceum, whereas those found in V. erubescens specimens were around 700 µm. Qualitative chemical screening indicated that phytosterols, triterpenoids, and phenolic compounds and their glycosides were among the commonly found phytoconstituents.
Comparative micromorphological and phytochemical studies on the roots of three Viburnum (Caprifoliaceae) species
In this study, the roots of Viburnum punctatum Buch.-Ham. ex D.Don, Viburnum coriaceum Blume, and Viburnum erubescens Wall. ex DC. were collected from the Nilgiri and Coimbatore hills in Tamil Nadu, India. Transverse sections of plant roots were prepared with the aid of a rotary microtome. The sections, each at a thickness of 10 µm, were stained and fixed in Canada balsam and the morphoanatomical features of each specimen were noted. The specimens were powdered using a mechanical grinder and were mounted in suitable media for starch grains, sclereids, druses, fibres, and vessels. Morphoanatomical features of diagnostic importance were recorded under different magnifications, followed by the determination of dimension and histological features of root components using a calibrated eyepiece micrometer and a polariser. The starch grains averaged 12, 17, and 20 µm in diameter in V. erubescens, V. punctatum, and V. coriaceum, respectively. The fibres were about 1 mm long in V. punctatum and V. coriaceum, whereas those found in V. erubescens specimens were around 700 µm. Qualitative chemical screening indicated that phytosterols, triterpenoids, and phenolic compounds and their glycosides were among the commonly found phytoconstituents.
___
- Altun ML, Saltan Çitoğlu G, Sever Yılmaz B & Özbek H (2009). Antinociceptive and anti-infl ammatory activities of Viburnum opulus. Pharm Biol 47: 653-658.
- Angiosperm Phylogeny Group (2009). An update of the Angiosperm Phylogeny Group classifi cation for the orders and families of fl owering plants: APG III. Bot J Linn Soc 161: 105-121.
- Babu K, Gokul Shankar S & Rai S (2010). Comparative pharmacognostic studies on the barks of four Ficus species. Turk J Bot 34: 215-224.
- CSIR (2003). Th e Wealth of India: A Dictionary of Indian Raw Materials and Industrial Products – Raw Material Series, Vol. 10, pp. 437-446. New Delhi: Council of Scientifi c and Industrial Research.
- Easu K (1964). Plant Anatomy. New York: John Wiley and Sons.
- Fukuyama Y, Kubo M, Minami H, Yuasa H, Matsuo A, Fujii T, Morisaki M & Harada K (2005). Rearranged vibsane-type diterpenes from Viburnum awabuki and photochemical reaction of vibsanin-B. Chem Pharm Bull 53: 72-80.
- Gamble JS (1935). Flora of the Presidency of Madras, Vol. I. Calcutta, India: Botanical Survey of India.
- Güvenç A & Duman H (2010). Morphological and anatomical studies of annual taxa of Sideritis L. (Lamiaceae), with notes on chorology in Turkey. Turk J Bot 34: 83-104.
- Hoerhammer L, Wagner H & Reinhardt H (1965). Isolation of fl avonoids from the barks of Viburnum prunifolium Dent. Apothekerzer 105: 1371.
- Johansen DA (1940). Plant Microtechnique. New York: McGraw-Hill Book Co.
- Khosa RL, Wahi AK, Mohan Y & Ray AB (1979). Isolation of Bergenin from roots of Viburnum nervosum Hook. Indian J Pharm 41: 120.
- Lala PK (1981). Practical Pharmacognosy, Vol. 1, pp. 136-153. India: Lina Guha Publication.
- Mutlu B (2010). New morphological characters for some Erysimum (Brassicaceae) species. Turk J Bot 34: 115-121.
- Nadkarni KM (2002). Indian Materia Medica, Vol. 1, pp. 1271-1272. Bombay, India: Popular Prakashan.
- Prabhu K, PK Karar, K Ponnudurai & S Hemalatha (2009). Pharmacognostic and preliminary phytochemical investigations on the leaves of Viburnum punctatum Buch.- Ham. ex D.Don. J Pharm Sci Res 1: 43-50.
- Sass JE (1940). Elements of Botanical Microtechnique. New York: McGraw-Hill Book Co.
- Scott FI (1950). Polyploidy among plant species extraneous in Indiana. Butler University Botanical Studies 9: 177-185.
- Tomassini L, Brkic D, Foddai S & Nicoletti M (1997). Iridoid glucosides from Viburnum rhytidophyllum. Phytochemistry 44: 751-753.
- Tomassini L, Gao J, Foddai S, Serafi ni M, Ventrone A & Nicoleti (2006). Iridoid glycosides from Viburnum chinshanense. Nat Prod Res 20: 697-700.
- Wallis TE (2005). Text Book of Pharmacognosy, pp. 559-618. New Delhi, India: CBS Publishers and Distributors.