Palavan Narçin ÜNSAL, Ayşin ÇOTUK, Kalaç Yeşim MARTINI

$alpha$ difluoromethylornithine inhibits the growth of fungus Macrophomina phaseoli

Ornitin dekarboksilaz (ODC) enziminin spesifik inhibitörü -difluorometilornitinin (DFMO) 0,1, 1,0 ve 2,0 mM konsantrasyonlarda Macrophomina phaseoli (Tassi) Goidanich mantarında misel büyümesini kayda değer bir şekilde inhibe ettiği saptandı. Besleyici ortama 0,25 mM konsantrasyonda putresin (Put) ilave edildiğinde ise DFMO nun ket vurucu etkisinin azaldığı belirlendi. Bu bulgular sonucunda poliaminlerin mantar büyümesinde esas işleve sahip olduğu ve fitopatojenik mantarların neden olduğu ürün kaybının önlenmesi amacıyla DFMO nun kullanabilirliği ortaya çıkmış olmaktadır.

$alpha$ difluorometilornitinin Macrophomina phaseoli mantarının büyümesine ket vurması

$alpha$ -Difluoromethylornithine (DFMO), a specific enzyme activated inhibitor of ornithine decarboxylase (ODC), significantly inhibited the mycelial growth of the fungus Macrophomina phaseoli (Tassi) Goidanich. Putrescine (Put), when added to the nutrient medium at a concentration of 0.25 mM, decreased the inhibitory effect of DFMO. These results suggest that polyamines (PAs) are essential for the growth of fungi and that DFMO is applicable to the alleviation or prevention of crop losses due to phytopathogenic fungi.

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