Gökhan CORAL

99107

The Isolation and Characterization of Glucoamylase Enzyme of an Aspergillus niger Natural Isolate

An extracellular glucoamylase enzyme was isolated from Aspergillus niger Z10 natural isolate in a liquid Czapek Dox medium which contained soluble starch (2%) as the sole carbon source. The molecular weight of the enzyme was found to be about 100.000 Daltons through Starch-SDS Polyacrylamide Gel Electrophoresis. The optimal pH range of the enzyme was found to be 4.5-5.5 and it was determined that the thermal stability of this enzyme is quite high.

Anahtar Kelimeler:

Aspergillus niger, Glucoamylase.

The Isolation and Characterization of Glucoamylase Enzyme of an Aspergillus niger Natural Isolate

An extracellular glucoamylase enzyme was isolated from Aspergillus niger Z10 natural isolate in a liquid Czapek Dox medium which contained soluble starch (2%) as the sole carbon source. The molecular weight of the enzyme was found to be about 100.000 Daltons through Starch-SDS Polyacrylamide Gel Electrophoresis. The optimal pH range of the enzyme was found to be 4.5-5.5 and it was determined that the thermal stability of this enzyme is quite high.

Keywords:

Aspergillus niger, Glucoamylase.,

Turkish Journal of Biology-Cover

ISSN: 1300-0152
Yayın Aralığı: 6
Yayıncı: TÜBİTAK

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