Morphological and 18S rRNA analysis of coccoid green algae isolated from lakes of Kızılırmak Delta

Molecular techniques are used today in many areas and have increased the confidence level of studies. The aim of the study was to compare the classical morphological characters of coccoid green algae under field and culture conditions by using modern molecular analysis. To achieve this aim, we first isolated the coccoid green algae from Liman and Cernek lakes. The morphological variations of green algae were observed under culture conditions and the phylogenetic relationships of these strains were defined according to the 18S rRNA gene sequences. According to sequence analysis, 2 of the isolated species had high similarity to Scenedesmus subspicatus (98%) and Desmodesmus sp. (100%).

Anahtar Kelimeler:

Key words: Coccoid green algae, Scenedesmus subspicatus, molecular analysis, Desmodesmus sp., sequence analysis

Morphological and 18S rRNA analysis of coccoid green algae isolated from lakes of Kızılırmak Delta

Keywords:

Key words: Coccoid green algae, Scenedesmus subspicatus, molecular analysis, Desmodesmus sp., sequence analysis,

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Callieri C, Stockner JG. Freshwater autotrophic picoplankton: a review. J Limnol 61: 1-14, 2002.
Fawley MW, Fawley KP, Buchheim MA. Molecular diversity among communities of freshwater microchlorophytes. Microb Ecol 48: 489-499, 2004.
Fawley MW, Fawley KP, Hoff man MJ. Diversity of coccoid algae in Minnesota and North Dakota Lakes. J Phycol 38: 8, 2002.
Lewis LA, McCourt RM. Green algae and the origin of land plants. Am J Bot 91: 1535-1556, 2004.
Kessler E, Schafer M, Hümmer C et al. Physiological, biochemical, and molecular characters for the taxonomy of the subgenera of Scenedesmus (Chlorococcales, Chlorophyta). Botanica Acta 110: 244-250 1997.
Hanagata N. Phylogeny of the subfamily Scotiellocystoideae (Chlorophyceae, Chlorophyta) and related taxa inferred from 18S ribosomal RNA gene sequence data. J Phycol 34: 1049- 1054, 1998.
Doyle JJ, Doyle JL. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19: 11-15, 1987. 8. Cullings KW. Design and testing of a plant-specifi c PCR primer for ecological and evolutionary studies. Mol Ecol 1: 233-240, 1992.
Medlin M, Elwood HJ, Stickel S et al. Th e characterization of enzymatically amplifi ed eukaryotic 16S-like rRNA-coding regions. Gene 71: 491-499, 1988.
Coleman AW, Suarez A, Goff L. Molecular delineation of species and syngens in Volvocacean green algae (Chlorophyta). J Phycol 30: 89-90, 1994.
Komárek J, Fott B. Chlorophyceae (Grünalgen) Ordnung: Chlorococcales. In: Huber-Pestalozzi G. ed. Das Phytoplankton des Süßwassers. E. Schweizerbart’sche Verlagsbuchhandlung; 1983: pp. 628-647.
Altschul SF, Madden TL, Schäff er AA et al. Gapped BLAST
and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25: 3389-402, 1997.
John DM, Whitton BA, Brook AJ. Th e Freshwater Algal Flora of the British Isles: An Identifi cation Guide to Freshwater and Terrestrial Algae. Cambridge University Press. Cambridge; 2003.
Fott B, Lochhead R, Clémençon H. Taxonomie der Arten Chlorella ultrasquamata Clém. et Fott und Chlorella fusca Shih. and Krauss. Arch Protistenk 117: 228-296, 1975.
Hegewald E. Th e Scenedesmus strains of the culture collection at Austin, Texas (UTEX). Arch. Hydrobiol/Suppl. 82 Algol Stud 48: 309-312, 1989.
Hegewald E, Schnepf E. Scenedesmus abundans (Kirchn.) Chod., an older name for Chlorella fusca Shih. et Krauss. Arch Protistenk 139: 133-176, 1991.
Hegewald E. Taxonomy and Phylogeny of Scenedesmus. Algae (Th e Korean Journal of Phycology) 12: 235-246, 1997.
Johnson JL, Fawley MW, Fawley KP. Th e diversity of Scenedesmus and Desmodesmus (Chlorophyceae) in Itasca State Park, Minnesota, USA. Phycologia 46: 214-229, 2007.
Li WH. Molecular Evolution. Sinauer Associates. Sunderland, MA, USA; 1997.
Tüney İ, Sukatar A, Pehlivan S. İzmir Körfezi’nde Gelişen Bir Kahverengi Alg Olan Cystoseira mediterranea Sauvageau’dan DNA İzolasyonu.Ulusal Su Günleri 28-30 Eylül 2005 Trabzon. Türk Sucul Yaşam Dergisi (Düzgüneş E, Okumuş İ, Öğüt H, eds.) - Yıl: 3, Sayı: 4, Sayfa: 100-104, 2005.
Tüney İ, Sukatar A. DNA extraction protocol from brown algae. Biol Divers Conserv 3: 51-55, 2010.