Saifullah KHAN, Farman ULLAH, Tahir MAHMOOD

In vitro antimicrobial and cytotoxic activity of Tamarix dioica Roxb. leaves

Medicinal plants have been in use for thousands of years for treatment of different types of diseases and a large inventory of useful drugs has been isolated from them. The aim of our study is to investigate a new and effective plant possessing antimicrobial activity. The crude extracts of Tamarix dioica Roxb. leaves, as well as fractions of the extract in various solvents, were investigated against standard strains of bacteria Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, Shigella flexneri, Bacillus subtilis, Salmonella typhi, and Staphylococcus aureus and fungi Candida glabrata, Aspergillus niger, Trichophyton rubrum, Candida tropicalis, and Aspergillus fumigatus. For in vitro antibacterial and antifungal studies of T. dioica extracts, the agar well diffusion technique was used. For cytotoxic study, brine shrimp (Artemia salina) larvae were used. Results of the crude extract and some fractionated samples showed significant antifungal properties, but low antibacterial response and negligible cytotoxic activity. It was recognized that Tamarix dioica has the potential for future development of new antifungal drugs/medicine.

Anahtar Kelimeler:

Key words: Tamarix dioica, antifungal, antibacterial, cytotoxic activity

In vitro antimicrobial and cytotoxic activity of Tamarix dioica Roxb. leaves

Keywords:

Key words: Tamarix dioica, antifungal, antibacterial, cytotoxic activity,

PDF

___

Morrison J, Coromilas J, Robbins M et al. Digitalis and myocardial infarction in man. Circulation 62: 8–16, 1980.
Gamboe RW, Chavez P, Baker WP. Antimicrobial activity of natural plant extracts against five bacterial species. J Ariz Nev Acad Sci 40: 165–167, 2008.
Khan R, Islam B, Akram M et al. Antimicrobial activity of five herbal extracts against multi drug resistant (MDR) strains of bacteria and fungus of clinical origin. Molecules 14: 586–597, 200 Baum BR. Introduced and naturalized tamarisks in the United States and Canada. Baileya 15: 19–25, 1967.
Qadry SMJS. Botanical identity and pharmacognosy of jhau Tamarix dioica. Ind J Pharmacy 31: 172, 1969.
Khan SU, Khan GM, Mehsud SUK et al. Antifungal activities of Tamarix dioica - An in vitro study. G J Med Sci 2: 40–42, 2004.
Khan S, Khan GM. In vitro antifungal activity of Rhazya stricta. Pak J Pharm Sci 4: 274–279, 2007.
Rojas R, Bustamante B, Bauer J et al. Antimicrobial activity of selected Peruvian medicinal plants. J Ethnopharmacol 88: 199–204, 2003.
Teyeb H, Zanina N, Neffati M et al. Cytotoxic and antibacterial activities of leaf extracts of Astragalus gombiformis Pomel (Fabaceae) growing wild in Tunisia. Turk J Biol 36: 53–58, 20 Nobmann P, Bourke P, Dunne J et al. In vitro antimicrobial activity and mechanism of action of novel carbohydrate fatty acid derivatives against staphylococcus aureus and MRSA. J Appl Microbiol 108: 2152–2161, 2010.
Hufford CD, Funderburk MJ, Morgan JM et al. Two antimicrobial alkaloids from heartwood of Liriodendron tulipifera. Inter J Pharm Sci 64: 789–792, 1975.
Chopade VV, Tankar AN, Ganjiwale RO et al. Antimicrobial activity of Capparis zeylanica Linn roots. Int J Green Pharmacy 2: 28–30, 2008.
Bastos MLA, Lima MRF, Conserva LM et al. Studies on the antimicrobial activity and brine shrimp toxicity of Zeyheria tuberculosa (Vell.) Bur. (Bignoniaceae) extracts and their main constituents. Ann Clin Micro Antimicrob 8: 8–16, 2009.
Mavi A, Yiğit N, Yiğit D et al. Antioxidant and antimicrobial activity of Turkish endemic Sonchus erzincanicus extracts. Turk J Biol 35: 243–250, 2011.
Brantner A, Grein E. Antibacterial activity of plant extracts used externally in traditional medicine. J Ethnopharmacol 44: 35–40, 1994.
Khan A, Rahman M, Islam S. Antibacterial, antifungal and cytotoxic activities of tuberous roots of Amorphophallus campanulatus. Turk J Biol 31: 167–172, 2007.
National Committee for Clinical Laboratory Standards. Reference method for broth dilution antifungal susceptibility testing of yeasts. Approved standard. Document M27-A. NCCLS. Wayne, PA, USA; 1997.
Parekh J, Chanda S. In vitro antibacterial activity of the crude methanol extract of Woodfordia fructicosa Kurz flower (Lythraceae). Braz J Microb 38: 204–207, 2007.
Nair R, Kalariya T, Chanda S. Antibacterial activity of some selected Indian medicinal flora. Turk J Biol 29: 41–47, 2005.
Morshed MA, Uddin A, Rahman A et al. In vitro antimicrobial and cytotoxicity screening of Terminalia arjuna ethanol extract. Int J Bioscienc 1: 31–38, 2011.
Finy DJ. Probit Analysis, 3rd ed. Cambridge University Press. Cambridge; 1971.
Somchit MN, Reezal I, Nur IE et al. In vitro antimicrobial activity of ethanol and water extracts of Cassia alata. J Ethnopharmacol 84: 1–4, 2003.
Bonjar S. Evaluation of antibacterial properties of some medicinal plants used in Iran. J Ethno Pharmacol 94: 301–305, 200 Sharma B, Kumar P. Extraction and pharmacological evaluation of some extracts of Tridax procumbens and Capparis deciduas. Int J App Res Nat Prod 1: 5–12, 2008.
Korir RK, Mutai C, Kiiyukia C et al. Antimicrobial activity and safety of two medicinal plants traditionally used in Bomet District of Kenya. Res J Med Plant 6: 370–382, 2012.
Parmar VS, Bisht KS, Sharma SK et al. Highly oxygenated bioactive flavones from Tamarix. Phytochemistry 36: 507–511, 19