Mujeebur Rahman KHAN, Shabbir ASHRAF, Farahnaz RASOOL, Khalid Mahmood SALATI

Field performance of Trichoderma species against wilt disease complex of chickpea caused by Fusarium oxysporum f. sp. ciceri and Rhizoctonia solani

The effects of Trichoderma harzianum, T. hamatum, T. viride, T. polysporum, and T. koningii on the wilt disease complex of chickpea caused by Fusarium oxysporum f. sp. ciceri and Rhizoctonia solani were investigated under field conditions during 2 consecutive years. Chickpea cultivar Avrodhi, grown in plots inoculated with F. oxysporum f. sp. ciceri and R. solani, showed chlorosis of leaves and wilting of foliage and exhibited a 22%-25% decrease in yield. Soil application of biocontrol agents (BCAs) checked the severity of wilt by 25%-56% and 39%-67% and increased the yield of chickpea by 12%-28% and 8%-24% in the 2 years, respectively. The disease control and yield enhancement were highest with T. harzianum, followed by T. hamatum and T. viride. Carbendazim treatment suppressed the disease by 20%-24%, leading to a 23%-28% improvement in the yield of chickpea. The BCAs established in the soil, and their rhizosphere populations, increased in the pathogen-infested plots. The study has demonstrated that T. harzianum, T. hamatum, and T. viride may perform as well as fungicide against wilt disease in chickpeas.

Anahtar Kelimeler:

Disease management, Trichoderma spp., Fusarium oxysporum f. sp. ciceri, nodulation, Rhizoctonia solani, fungicides

Field performance of Trichoderma species against wilt disease complex of chickpea caused by Fusarium oxysporum f. sp. ciceri and Rhizoctonia solani

Keywords:

Disease management, Trichoderma spp., Fusarium oxysporum f. sp. ciceri, nodulation, Rhizoctonia solani, fungicides,

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Abd-El-Khair H, Khalifa RKM, Haggag KHE (2010). Effect of Trichoderma species on damping off diseases incidence, some plant enzymes activity and nutritional status of bean plants. J Am Sci 6: 486–497.
Akrami A, Ibrahimov AS, Zafari DM, Valizadeh E (2009). Control Fusarium rot of bean by combination of by Trichoderma harzianum and Trichoderma asperellum in greenhouse condition. Agr J 4: 121–123.
Andrabi M, Vaid A, Razdan VK (2011). Evaluation of different measures to control wilt causing pathogens in chickpea. J Plant Prot Res 51: 5–59.
Bhatti MA, Ali S, Khan IU (1987). Pathogenicity of fungi associated with wilt of chickpea with special reference to Verticillium albo-atrum. Pakistan J Agr Res 8: 39–42.
Bruckner H, Przybylski M (1984). Isolation and structural characterization of polypeptides antibiotics of the peptaibol class by high-performance liquid chromatography with field desorption and fast atom bombardment mass spectrometry. J Chromatogr 296: 263–275.
Chattopadhyay SB, Sen Gupta PK (1967). Studies on wilt diseases of pulses I. Variation and taxonomy of Fusarium spp. associated with the wilt disease of pulses. Indian J Mycol Res 5: 45–53.
Chet I, Baker R (1981). Isolation and biocontrol potential of Trichoderma hamatum from soil naturally suppressive of Rhizoctonia solani. Phytopathology 71: 286–290.
Dubey SC, Tripathi A, Singh B (2012). Combination of soil application and seed treatment formulations of Trichoderma species for integrated management of wet root rot caused by Rhizoctonia solani in chickpea (Cicer arietinum ). Indian J Agr Sci 82: 356–362.
Ganesan S, Kuppusamy RG, Sekar R (2007). Integrated management of stem rot disease (Sclerotium rolfsii) of groundnut (Arachis hypogaea L.) using rhizobium and Trichoderma harzianum (ITCC - 4572). Turk J Agr For 31: 103–108.
Haware MP, Nene YL, Natarajan M (1996). The survival of Fusarium oxysporum f. sp. ciceri in soil in the absence of chickpea. Phytopathol Mediterr 35: 19–12.
Hussain S, Ghaffar A, Aslam M (1990). Biological control of Macrophomina phaseolina charcoal rot of sunflower and mung bean. J Phytopathol 130: 157–160.
Jalali BL, Chand H (1992). Chickpea wilt. In: Singh US, Mukhopadyayas AN, Kumar J, Chaube HS, editors. Diseases of International Importance, Vol 1. Upper Saddle River, NJ, USA: Prentice Hall, pp. 429–464.
Jeffries P, Young TWK (1994). Interfungal Parasitic Relationship. Wallingford, UK: CAB International.
Kaur NP, Mukhopadhyay AN (1992). Integrated control of chickpea wilt complex by Trichoderma and chemical methods in India. Trop Pest Manag 38: 372–375.
Kerik DC (2009). A rapid method to quantify fungicide sensitivity in the brown rot pathogen Monolinia fructicola. Plant Dis 93: 328–331.
Khan MR, Anwer MA, Shahid S (2011). Management of gray mould of chickpea, Botrytis cinerea with bacterial and fungal biopesticides using different modes of inoculation and application. Biol Cont 57: 13–23.
Khan MR, Gupta J (1998). Antagonistic efficacy of Trichoderma species against Macrophomina phaseolina on eggplant. J Plant Dis Prot 105: 387–393.
Khan MR, Khan N, Khan SM (2001). Evaluation of agricultural materials as substrate for mass culture of fungal biocontrol agents of fusarial wilt and root knot nematode diseases. Test Agrochem Culti 22: 50–51.
Khan MR, Khan SM, Mohiddin FA (2004). Biological control of Fusarium wilt of chickpea through seed treatment with the commercial formulation of Trichoderma harzianum and Pseudomonas fluorescens. Phytopathol Mediterr 43: 20–25.
Kumar M, Jain AK, Kumar P, Chaudhary S, Kumar S (2008). Bio- efficacy of Trichoderma spp. against management of chickpea damping-off caused by Rhizoctonia solani Kuhn. Plant Arch 8: 399–400.
Lifshitz R, Windam MT, Baker R (1986). Mechanism of biological control of preemergence damping-off of pea by seed treatment with Trichoderma species. Phytopathology 76: 720–725.
Lorito P, Emerson OH, Lomas N (1993). The isolation of toxic substances from the culture filtrate of Trichoderma. Phytopathology 26: 1068–1068.
Marten P, Bruckner S, Luth P (1999). Plant growth promotion of different cultivated plants and biological control of soil-borne phytopathogenic fungi by Bacillus subtilis strain B2g. J Plant Dis Prot 106: 74–81.
Mohiddin FA, Khan MR, Khan SM (2010). Why Trichoderma is considered super hero (super fungus) against the evil parasites? Plant Pathol J 9: 92–102.
Nautiyal CS (1997). Selection of chickpea-rhizosphere-competent Pseudomonas fluorescens NBRI 1303 antagonistic to Fusarium oxysporum f. sp. ciceri, Rhizoctonia bataticola and Pythium sp. Curr Microbiol 35: 52–58.
Nene YL, Thapliyal PN (1993). Fungicides in Plant Disease Control. New Delhi, India: Oxford and IBH.
Papavizas GC, Dunn MT, Lewis JA, Beagle-Ristaino J (1984). Liquid fermentation technology for experimental production of biocontrol fungi. Phytopathology 74: 1171–1175.
Prasad RD, Rangeshwaran R, Anuroop CP, Rashmi HJ (2002). Biological control of wilt and root rot of chickpea under field conditions. Ann Plant Prot Sci 10: 72–75.
Rizvi R, Mahmood I, Tiyagi S A, Khan Z (2012). Effect of some botanicals for the management of plant-parasitic nematodes and soil-inhabiting fungi infesting chickpea. Turk J Agr For 36: 710–719.
Rudresh DL, Shivaprakash MK, Prasad R D (2005). Potential of Trichoderma spp. as biocontrol agents of pathogens involved in wilt complex of chickpea (Cicer arietinum L.). J Biol Control 19: 157–166.
Singh RS (2005). Plant Diseases. New Delhi, India: Oxford and IBH.
Tomar OK, Singh D, Singh D (2010). Stability for yield and related traits in chickpea (Cicer arietinum). Indian J Agric Sci 80: 1076–1080.
Toyoda H, Utsumi R (1991). Method for the Prevention of Fusarium Diseases and Microorganisms Used for the Same. US Patent No. 4 988 586.
Waksman SA (1922). A method of counting the number of fungi in the soil. J Bacteriol 7: 339–341.
Xiao CL, Subbarao KV (1998). Relationships between Verticillium dahliae inoculum density and wilt incidence, severity, and growth of cauliflower. Phytopathology 88: 1108–1115.
Yedidia I, Benhamou N, Chet I (1999). Induction of defense responses in cucumber plants (Cucumis sativus L.) by the biocontrol agent Trichoderma harzianum. Appl Environ Microbiol 65: 1061–1070.