Xanthomonas gardneri and X. vesicatoria are the causal agents of bacterial spot of tomato in Bulgaria. X. gardneri was identified in the aria of our country on variety Bela for the first time in 2010. Symptoms on the leaves were dark, circular to irregular, water-soaked spots surrounded by chlorotic halos on fruit formed scabs. X. vesicatoria and X. gardneri can be identified through bacterial isolation only. X. gardneri was gram-negative, aerobic rods with a single flagellum. Bacterial colonies on peptone sucrose agar were yellow and rose with smooth margins. Starch and pectate hydrolysis tests were not positive. BIOLOGTM GN2 (Biolog, Inc., Hayward, CA, USA) microplates were used for obtaining metabolic fingerprints. The metabolic profile of tomato isolates with bacterial spot symptoms was typical for X. gardneri. The distribution of X. gardneri on tomato crops around the country required a research on the resistance of the Bulgarian tomato varieties. Immune tomato varieties were not identified. Resistant genotypes (with mean score 0.01-0.60) were Nikolina F1 -determinate large-fruit variety for mid-early field production and IZK Alya - variety of cherry type. Highly sensitive (with mean score over 3.0) were two determinate varieties - Bela and Venera, suitable for industrial processing