Atatürk Baraj Gölü'nde Yaşayan Carasobarbus luteus (H., 1843) ve Carassius carassius (L., 1758) Balıklarının Spermatolojik Özelliklerinin Karşılaştırılması

Bu çalışmanın amacı, Carasobarbus luteus (Heckel, 1843) ve Carassius carassius (L., 1758) balıklarının başlıca spermatolojik özelliklerinin belirlenmesidir. Spermalar üreme sezonunda, 30 adet C. luteus ve 20 adet C. carassiustan abdominal masaj yöntemi ile alındı. Alınan spermalarda, miktar, motilite, motilite süresi, yoğunluk ve pH belirlendi. Çalışmadaki C. luteus ve C. carassius balığından alınan sperma miktarı, spermatozoa motilite oranı, spermatozoa motilite süresi, spermatozoa yoğunluğu ve sperma pHı sırasıyla ortalama 0,72±0,08 ml ve 1,03±0,20 ml; %57,67±3,67 ve %81,00±1,43; 190,30±11,25 sn ve 107,30±12,03 sn; 13,15±1,33 x109 /ml ve 10,53±1,28x109 /ml; 8,13±0,11 ve 7,60±0,04 olarak belirlendi. Söz konusu iki türün spermatozoa motilite oranı, motilite süresi, spermatozoa yoğunluğu ve sperma pHı arasında önemli farklılıklar gözlemlendi.

Comparation of Spermatological Characteristics in Carasobarbus luteus (H., 1843) and Carassius carassius (L., 1758) Living in Atatürk Dam Lake

The aim of this study was to determine the main spermatological properties in Carasobarbus luteus (H., 1843) and Carassius carassius (L., 1758) in Atatürk Dam Lake. Sperm was collected by abdominal massage from thirty C. luteus and twenty C. carassius. Milt volume (ml), spermatozoa motility (%), motility duration (s), spermatozoa concentration (x109 /ml) and pH in C. luteus and C. carassius were 0.72±0.08 ml and 1.03±0.20 ml, %57.67±3.67 and % 81.00±1.43, 190.30±11.25 s and 107.30±12.03 s, 13.15±1.33 and 10.53±1.28x109 /ml, 8.13±0.11 and 7.60±0.04, respectively. The spermatozoa motility, motility duration, spermatozoa concentration and pH was found significant compared with fish species group.

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Ahmed HA, Al-Mukhtar MA, Al-Adhub HY. 1984. The reproductive biology of Carasobarbus luteus (Pisces, Cyprinidae) in Al-Hammar Marsh, Iraq. Cybium, 8: 69-80.
Al Hazzaa R, Hussein A. 2003. Initial observations in himri (Barbus luteus, Heckel) propagation. Turk. J. Fish. Aqua. Sci., 3: 41-45.
Al-Daham NK, Bhatti MN. 1979. Annual changes in the ovarian activity of the freshwater teleost Barbus luteus (Heckel) from Southern Iraq. J. Fish Biol., 14: 381-387.
Amin F, Milad K, Amir T, Mohammad S. 2011.Effects of clove extract as an anesthetic on sperm motility traits and some hematological parameters in Prussian carp Carassius gibelio. Advances in Environmental Biology.
http://www.thefreelibrary.com/Advances+in+Environmental +Biology/2011/ May/1-p52430.
Akcay E, Bozkurt Y, Seçer S, Tekin N. 2004. Cryopreservation of mirror carp semen. Turkish Journal of Veterinary and Animal Sciences 28: 837-843.
Alavi SMH, Linhart O, Coward K, Rodina M. 2008. Fish spermatology: implications for aquaculture management. Pages 397-460 in Alavi SMH, Cosson J, Coward K, Rafiee G. editors. Fish spermatology. Alpha Science International Ltd., Oxford, United Kingdom.
Alavi SMH, Jorfi E, Hatef A, Mortezavi SAS. 2010. Sperm motility and seminal plasma characteristics in Barbus sharpeyi (Gunther, 1874). Aquaculture Research, 41: 688- 694.
Aral F., Şahinöz, E., Doğu Z., Demirkol R. 2004. Atatürk Baraj Gölündeki Carasobarbus luteus (Heckel, 1843)un spermatolojik özelliklerinin belirlenmesi, Süleyman Demirel Üniversitesi, Eğirdir Su Ürünleri Fakültesi Dergisi, 2: 72-77.
Baker TT, Timmons LS. 1991. Precision of ages estimated from five bony structure of arctic char (Salvelinus alpinus) from the Wood River System, Alaska. Canadian Journal of Fisheries and Aquatic Sciences, 48: 1007-1014.
Becer ZA, Kır İ, Çubuk H. 1998. Some Reproductive Characteristics of Carassius carassius L., 1758 (IspartaBurdur) in the Karacaören-I Dam Lake (in Turkish). XIV National Biology Congress 710 September, Samsun, Turkey, Volume II, 126138.
Billard R, Cosson J, Perchec G, Linhart O. 1995. Biology of sperm and artificial re-production in carp, Aquaculture, 124: 95-112. doi:10.1016/0044-8486(94)00231-C.
Bozkurt Y, Öğretmen F, Seçer FS. 2009. Effect of different extenders and storage periods on motility and fertilization success of grass carp (Ctenopharyngodon idella) sperm during spawning season. Agricultural Science Journal, 15: 277-284.
Bromage RN, Roberts JR. 1995. Broodstock management and egg larval quality. Blackwell Science Ltd., Cambridge, 424 p. Brown DR, Shrable JB, Orr WH. 1994. The use of various fertilization media and their effects on rainbow trout gametes. Developments in fish culture. http://www.fws.gov/ennis/investigations/the%20use%20of% 20varıous%20fertilization%20media%20and%20their%20ef fects.pdf
Cabrita E, Anel L, Herraéz PM. 2001. Effect of external cryoprotectans as membrane stabilizers on cryopreserved trout sperm. Theriogenology, 56: 623635.
Chowdhury I, Joy KP. 2001. Seminal vesicle and testis secretions in Heteropneuste fossilis (Bloch): composition and effects on sperm motility and fertilisation. Aquaculture 193: 355 371.
Epler P, Sokolowska-Mikolajczyk M, Popek W, Bieniarz K, Kime DE, Bartel R. 1996. Gonadal development and spawning of Barbus sharpeyi, Barbus luteus and Mugil hishni in fresh and saltwater lakes in Iraq. Arch. Pol. Fish., 4: 113-124.
Fauvel C, Savoye O, Dreanno C, Cosson J, Suquet M. 1998. Characteristics of sperm of captive seabass (Dicentrarchus labrax L.) in relation to its fertilisation potential. Journal of Fish Biology, 54: 356-369.
Geffen AJ, Evans JP. 2000. Sperm traits and fertilisation success of male and sex-reversed female rainbow trout (Oncorhynchus mykiss). Aquaculture 182: 6172.
Geldiay R, Balık S. 1988. Türkiye Tatlısu Balıkları (Ders Kitabı), Ege Üniversitesi, Fen Fakültesi Kitaplar Serisi, No: 97, Bornova İzmir, 519 s.
Holopainen IJ, Tonn WM, Paszkowski CA. 1997. Tales of two fish: the dichotomous biology of crucian carp (Carassius carassius (L.)) in Northern Europe. Ann. Zool. Fennici., 34: 1-22. Islam MS, Akhter T. 2011. Tale of fish sperm and factors affecting sperm motility: A Review. Advances in Life Sciences. 1: 11-19.
Jezierska B, Witeska M. 1999. The effect of time and temperature on motility of spermatozoa of common and grass carp. Elec J. Polish Agricult Univers 2 (2), #04. http://www.ejpau.media.pl/volume2/issue2/fisheries/art-04.html.
Kaspara V, Kohlmann K, Vandeputtec M, Rodinaa M, Gelaa D, Kocoura M, Alavia SMH, Hulaka M, Linhart O. 2007.
Equalizing sperm concentrations in a common carp (Cyprinus carpio) sperm pool does not affect variance in proportions of larvae sired in competition. Aquaculture. 272, Suppl. 1, 204209.
Lahnsteiner F, Mansour N, McNiven MA, Richardson GF. 2009. fatty acids of rainbow trout (Oncorhynchus mykiss) semen: Composition and effects on sperm functionality. Aquaculture 298: 118-124.
Linhart O, Rodina M, Cosson J. 2000. Cryopreservation of Sperm in Common Carp Cyprinus carpio: Sperm Motility and Hatching Success of Embryos. Cryobiology, 41: 241250.
Olsén KH, Sawisky GR, Stacey NE. 2006. Endocrine and milt responses of male crucian carp (Carassius carassius L.) to periovulatory females under weld conditions. General and Comparative Endocrinology 149: 294302.
Perchec G, Jeulin C, Cosson J, André F, Billard R. 1995. Relationship between sperm ATP content and motility of carp spermatozoa. Journal of Cell Science 108: 747-753.
Perez MJ, Rodriguez C, Cejas JR, Martin MV, Jerez S, Lorenzo A. 2007. Lipid and fatty acid content in wild white seabream (Diplodus sargus) broodstock at different stages of the reproductive cycle. Comparative Biochemistry and Physiology Part B 146: 187-196.
Policar T, Podhorec P, Stejskal V, Kozák P, vinger V, Alavi SMH. 2011. Growth and survival rates, puberty and fecundity in captive common barbel (Barbus barbus L.) under controlled conditions. Czech J. Anim. Sci., 56: 433 442.
Rurangwa E, Kime DE, Ollevier F, Nash JP. 2004. The measurement of 466 sperm motility and factors affecting sperm quality in cultured fish. 467 Aquaculture, 234: 1-28.
Shafi S. 2012. Study on fecundity and GSI of Carassius carassius (Linneaus, 1758-introduced) from Dal Lake Kashmir, Journal of Biology, Agriculture and Healthcare, 2: 3.
Suquet M, Omnes MH, Normant Y, Fauvel C. 1992. Assessment of sperm concentration and motility in turbot Scophthalmus maximus, Aquaculture, 101: 177-85.
Şevik R, Yüksel M. 1997. Atarük Baraj Gölünde yaşayan Carasobarbus luteus (Heckel, 1843) üzerine araştırmalar-II, IX. Ulusal Su Ürünleri Sempozyumu, Isparta, 1719 Eylül.
Taghizadeh V, Imanpoor MR, Sadeghi A. 2013. Effect of Extenders and Different Concentrations of Methanol on Motility Parameters of Goldfish (Carassius auratus gibelio) Spermatozoa after Short-Term Storage. World J. Fish and Marine Sci., 5: 492-496.
Tarkan AS, Coppa GH, Zieba G, Godarda MJ, Cucherousset J. 2009. Growth and reproduction of threatened native crucian carp Carassius carassius in small ponds of epping forest, South-East England, Aquatic Conservation: Marine and freshwater ecosystems aquatic conserv: Mar. Freshw. Ecosyst,19: 797805.
Tekin N. 1990. Erkek üreme organlarının muayenesi (androlojik muaeyeneler). In: Alaçam, E. Ed., Theriogenoloji evcil hayvanlarda reprodüksiyon suni tohumlama obstetrik ve infertilite. Nurol Matbacılık, Ankara. 5367.s.
Tekin N, Seçer S, Akçay E, Bozkurt Y, Kayam S. 2003. The effect of age on spermatological properties in rainbow trout (Oncorhynchus mykiss W. 1792). Turkish Journal of Veterinary and Animal Scinces, 27: 3744.
Verma DK, Routray P, Dash C, Dasgupta S, Jena JK, 2009. Physical and Biochemical Characteristics of Semen and Ultrastructure of Spermatozoa in Six Carp Species. Turkish Journal of Fisheries and Aquatic Sciences 9: 67-76.
Zarski D, Horvath A, Bernath G, Palinska-Zarska K, Krejszeff S, Muller T, Kucharczyk D. 2014. Application of different activating solutions to in vitro fertilization of crucian carp, Carassius carassius (L.), eggs. Aquacult Int 22: 173184.