Turgay TAŞKIN, Çağrı KANDEMİR, Nedim KOŞUM, Güldehan BİLGEN, Raziye IŞIK

Saanen Keçilerinde ?- Laktoglobulin (?-LG) Geni Ekzon 7 Polimorfizmi ve Süt Verimi ile İlişkisi

Süt proteinlerinden biri olan ?-Laktoglobulin (?-LG), peynir yapımı gibi sütün teknolojik özellikleri üzerinde önemli bir fonksiyona sahiptir. Serum protein genleri ile süt verimi/bileşimi arasındaki ilişkiler daha önceki çalışmalarda araştırılmıştır. ?-LG, süt verimi ve protein kalitesini artırmak için seleksiyon ve ıslah programları için aday bir gen olarak kullanılabilmektedir. Bu çalışmanın amacı, ?-LG gen polimorfizmini ve ?-LG genotipleri ile süt verimi arasındaki ilişkiyi araştırmaktır. Bu çalışmada, PCR-RFLP yöntemi ile polimorfizm belirlenmesi amacıyla Avustralya kökenli toplam 74 safkan Saanen keçi kullanılmıştır. SacII restriksiyon enzimi ile ?-LG geni 427 baz çiftlik yedinci ekzonu (GenBank: Z33881.1) genotiplenmiştir. ?-LG/SacII lokusunda iki alel (S1, S2) ve üç genotip (S1S1, S1S2, S2S2) belirlenmiştir. ?- LG/S1S1, genotipi tek kesim bölgesine sahip olduğundan iki bant vermektedir (347 bç ve 80 bç). ?-LG/S1S2 heterozigot genotipi üç bant oluşturmaktadır (427 bp, 347 bp ve 80 bp). ?-LG/S2S2 genotipig.4601G> A pozisyonunda tek nükleotid değişiminden dolayı 427 bç uzunluğunda kesilmemiş ürün vermektedir. SS2 genotip frekansı (% 43.3) diğer genotiplerden daha yüksektir. S1 allel frekansı predominant olarak belirlenmiştir. Saanen ırkında Hardy-Weinberg dengesinde sapma gözlenmemiştir. ?-LG/ SacII lokusunda, genotipler ile laktasyon süt verimi arasında önemli bir ilişki bulunmamıştır. Ancak ?-LG/ S1S1 genotipinin daha yüksek laktasyon süt verimine sahip olduğu belirlenmiştir. ?-LG geninin süt verimi ve kompozisyonu gibi ekonomik özellikler için moleküler marker olarak kullanılabileceği sonucuna varılmaktadır

Polymorphism in Exon 7 of ?- Lactoglobulin (?-LG) Gene and Its Association with Milk Yield in Saanen Goats

B-Lactoglobulin (?-LG) is one of milk protein and has important function on technological properties of milk such as cheese making. The relations between whey protein genes and milk yield/ composition have been investigated in previous researches. ?-LG can be utilized as a candidate gene for selection and breeding programs to increase milk yield and protein quality. The aim of this study is to investigate the ?-LG gene polymorphism and relation between ?- LG genotypes and milk yield. In this study, a total of 74 purebred Saanen goats originated from Australia were used to detect polymorphism with PCR-RFLP method. SacII digestion in 427 bp of ?-LG exon 7 (GenBank: Z33881.1) was revealed. Two alleles (S1, S2) and 3 genotypes (S1S1, S1S2, S2S2) were determined in ?-LG/ SacII locus. The ?-LG/S1S1 genotype with only one restriction site revealed two bands (347 bp and 80 bp). The ?-LG/S1S2 heterozygote genotype generated three bands (427 bp, 347 bp and 80 bp). An undigested product, 427 bp was ?-LG/S2S2 genotype which was due to a single nucleotide substitution at position g.4601G>A. S1S2 with genotype frequency (43.3%) higher than the other genotypes. S1 allele frequency was determined predominantly. Deviation from Hardy-Weinberg equilibrium was not identified in the Saanen breed. In ?-LG/ SacII locus, a significant relationship was not found between genotypes and lactation milk yield. However, ?-LG/ S1S1 genotype was observed to have higher lactation milk yield. It is concluded that ?-LG gene could be used as a molecular marker for economic traits such as milk yield and composition.

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