Ahmet AYGÜN, Hatice DUMANOĞLU

Ayvada Cydonia vulgaris Pers. Olgunlaşmamış Çenek Yapraklardan Somatik Embriyo Oluşumu

Bu çal ışmada, Limon ayvas ı n ı n Cydonia vulgaris Pers. cv Limon serbest tozlanma sonucu olu ş muş tohumları n ı n olgunlaş mam ış çenek yapraklar ı tam çiçeklenmeden 7, 8, 9, 10 ve 11 hafta sonra ç ı karı lm ış ve 250 mg/I Lglutamin, 500 mg/I kasein hidrolisat, 0.0, 0.5, 1.0, 2.0 and 5.0 mg/I benziladenin BA , 0.0, 0.5, 1.0 ve 2.0 mg/I 2,4- diklorofenoksiasetik asit 2,4-D ya da naftalenasetik asit NAA ilave edilmi ş makro elment ve demir düzeyi 'A olan değ iştirilmiş Murashige ve Skoog MS ortam ı nda kültüre al ı nm ışt ı r. Çenek yapraklar 4 hafta süreyle karanl ı k koş ullarda inkübe edilmiş ve daha sonra hormon içermeyen 250 mg/I L-glutamin, 500 mg/I kasein hidrolisat ilave edilmi ş makro element ve demir düzeyi A olan de ğ i ştirilmiş MS ortamı nda karanl ı k koş ullarda 3 kez alt kültüre al ı nm ışt ı r. Her alt kültür sonras ı nda embriyonik çenek yaprak oran ı % ve somatik embriyo say ı s ı adet/çenek yaprak belirlenmi ştir. Ara şt ı rmada en yüksek embriyonik çenek yaprak oran ı %53.3 - 80.0 ve somatik embriyo say ı s ı 1.07±0.07 - 2.53±0.79 adet/ çenek yaprak , ba ş lang ı ç ortam ı nda 0.0 mg/I BA + 2.0 mg/ NAA, 0.5 mg/I BA + 0.5 mg/I 2,4-D, 0.5 mg/I BA + 1.0 mg/I NAA, 1.0 mg/I BA + 2.0 mg/I 2,4-D ya da 1.0 mg/I BA + 1.0 mg/I NAA kombinasyonlar ı kullan ı lm ış ve tam çiçeklenmeden 7 ve 11 hafta sonra kültüre al ı nm ış çenek yapraklardan elde edilmiştir

Anahtar Kelimeler:

Ayva, somatik embriyo olu şumu, benziladenin, naftalenasetik asit, 2, 4-diklorofenoksiasetik asit, L-glutamin, kasein hidrolisat

Somatic Embryogenesis from Immature Cotyledons in Quince Cydonia vulgaris Pers.

In this study, immature cotyledons of open-pollinated seeds from 'Limon' quince cuttivar Cydonia vulgaris Pers. cv 'Limon' were excised at 7, 8, 9, 10 and 11 weeks after anthesis and cultured on Modified Murashige and Skoog MS medium containing macroelements and iron supplemented with 250 mg/I L-glutamine, 500 mg/I casein hydrolysate , 0.0, 0.5, 1.0, 2.0 and 5.0 mg/I 6-benzyladenine BA and 0.0, 0.5, 1.0 and 2.0 mg/I 2,4- dichlorophenoxyacetic acid 2,4-D or naphthalene-acetic acid NAA . The cotyledons were incubated for 4 weeks in darkness, prior to subculturing on hormone-free MS medium containing A macroelements and iron supplemented with 250 mg/I L-glutamine, 500 mg/I casein hydrolysate in darkness for three times. Percentage of embryonic cotyledon % and number of somatic embryos number per cotyledon were determined after each subculture. In this research, the highest percentage of embryonic cotyledons 53.3 - 80.0% and number of somatic embryo 1.07±0.07 - 2.51:0.79 were obtained with 0.0 mg/I BA + 2.0 mg/ NAA, 0.5 mg/I BA + 0.5 mg/I 2,4-D, 0.5 mg/I BA + 1.0 mg/I NAA, 1.0 mg/I BA + 2.0 mg/I 2,4-D or 1.0 mg/I BA + 1.0 mg/I NAA combinations in induction medium and with cotyledons cultured at 7 and 11 weeks after anthesis.

Keywords:

Quince, somatic embryogenesis, benzyladenine, naphthalene-acetic acid, 2, 4-dichlorophenoxyacetic acid, L-glutamine, casein hydrolysate,

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