Role of H2O2 on photosynthetic characteristics of soybean genotypes under low water input

Soybean is subjected to abiotic stresses that immensely affect its productivity during its lifespan and threaten food security globally. Recent research recommends that chemical substances could be applied to plants as an alternative to traditional agriculture to better abide abiotic stresses. Hydrogen peroxide (H2O2) is a potential agent that can serve for this purpose. Up to today, responses of exogenous H2O2 on photosynthetic machinery in plants exposed to drought is poorly investigated. Therefore, the effects of exogenous low dose H2O2 on plant chlorophyll fluorescence in two soybean genotypes (Glycine max L. Merrill), 537 (tolerant) and 520 (susceptible), under drought were evaluated. Drought which we had found significantly reduced in two genotypes, did not cause change in Fv/Fm and ΦPSII of tolerant genotype, contrarily subsided qP and ETR values. However, Fv/Fm, ΦPSII, qP and ETR failed in susceptible genotypes under drought. Increases in NPQ were determined under stress in both genotypes. Exogenous H2O2 mitigated the drought-induced impairment in photosystem II efficiency in both genotypes. This data indicates that low dose H2O2 further enhanced the tolerance to drought via regulation of the photochemical process in both genotypes.Keywords: soybean, hydrogen peroxide, water scarcity, chlorophyll fluorescence

PDF

___

[1] DB. Lobell, W. Schlenker, J. Costa-Roberts, “Climate trends and global crop production since 1980”, Science, vol. 333, pp. 616-620, 2011.
[2] S. Fahad, A.A. Bajwa, U. Nazir, et al., “Crop Production under drought and heat stress: plant responses and management options”, Frontiers in Plant Science, vol. 8, p. 1147, 2017. [3] J. Flexas, A. Diaz-Espejo, J. Galmés, et al., “Rapid variations of mesophyll conductance in response to changes in CO2 concentration around leaves”, Plant Cell and Environment, vol. 30, pp. 1284-1298, 2007.
[4] S.H. Hung, C.W. Yu, C.H. Lin, “Hydrogen peroxide functions as a stress signal in plants”, Botanical bulletin of Academia Sinica, vol. 46, pp. 1-10, 2005.
[5] J.T. Li, Z.B. Qiu, X.W. Zhang, et al. “Exogenous hydrogen peroxide can enhance tolerance of wheat seedlings to salt stress”, Acta Physiologiae Plantarum, vol. 33, pp. 835-842, 2011.
[6] J. Dat, S. Vandenabeele, E. Vranová, et al., “Dual action of the active oxygen species during plant stress responses”, Cellular and Molecular Life Sciences, vol. 57, pp. 779- 795, 2000.
[7] X. Wang, C. Hou, J. Liu, et al., “Hydrogen peroxide is involved in the regulation of rice (Oryza sativa L.) tolerance to salt stress”, Acta Physiologiae Plantarum, vol. 35, pp. 891-900, 2013.
[8] F. Ashfaque, M.I.R., Khan, N.A., Khan, “Exogenously applied H2O2 promotes proline accumulation, water relations, photosynthetic efficiency and growth of wheat (Triticum aestivum L.) under salt stress”, Annual Review & Research in Biology, vol. 4, issue 1, pp. 105-120, 2014.
[9] S.M. Abass, H.I. Mohamed, “Alleviation of adverse effects of drought stress on common bean (Phaseolus vulgaris L.) by exogenous application of hydrogen peroxide”, Bangladesh Journal of Botany, vol. 41 pp. 75-83, 2011.
[10] Y. Ishibashi, H. Yamaguchi, T. Yuasa, et al., “Hydrogen peroxide spraying alleviates drought stress in soybean plants”, Journal of Plant Physiology, vol. 168, pp.1562-1667, 2011.
[11] P. Mehta, S.I. Allakhverdiev, A. Jajoo, “Characterization of photosystem II heterogeneity in response to high salt stress in wheat leaves (Triticum aestivum)”, Photosynthesis Research, vol. 105, pp. 249- 255, 2010.
[12] K. Zushi, N. Matsuzoe, “Using of chlorophyll, a fluorescence OJIP transients for sensing salt stress in the leaves and fruits of tomato”, Scientia Hortculture, vol. 219, pp. 216-221, 2017.
[13] T. Shaheen, M. Rahman, M.S. Ria, et al. “Abiotic and biotic stresses in soybean production soybean production: soybean production and drought stress”, vol. 1 pp. 177-196, 2016.
[14] Y.S. Ku, W.K. Au-Yeung, Y.L. Yung, et al. “Drought stress and tolerance in soybean, in a comprehensive survey of international soybean research” Genetics, Physiology, Agronomy and Nitrogen Relationships Board J. E., editor, New York, NY: InTech, pp. 209-237, 2013.
[15] N. Saruhan Guler, N. Pehlivan, “Exogenous low-dose hydrogen peroxıde enhances drought tolerance of soybean (Glycine max L.) through inducıng antioxidant system”, Acta Biologica Hungarica, vol. 67, issue 2, pp.169-183, 2016.
[16] D.I. Arnon, “Copper enzymes in chloroplasts, polyphenoloxidase in Beta vulgaris”, Plant Physiology, vol. 24, pp. 1- 15, 1949.
[17] E.M.J. Jaspars, “Pigmentation of tobacco crown gall tissues cultured in vitro in dependence of the composition of the medium”, Physiologia Plantarum, vol. 18, pp. 933-940, 1965.
[18] R. Terzi, A. Kadioglu, E. Kalaycioglu, et al., “Hydrogen peroxide pretreatment induces osmotic stress tolerance by influencing osmolyte and abscisic acid levels in maize leaves”, Journal of Plant Interactions, vol. 9, pp. 559-565, 2014.
[19] M. Ghorbanpour, M. Hatami, K. Khavazi, “Role of plant growth promoting rhizobacteria on antioxidant enzyme activities and tropane alkaloids production of Hyoscyamus niger under water deficit stress”, Turkish Journal of Biology, vol. 37, pp. 350-360, 2013.
[20] C. Pastenes, P. Pimentel, J. Lillo, “Leaf movements and photoinhibition in relation to water stress in field-grown beans”, Journal of Experimental Botany, vol. 56 pp. 425-433, 2005.
[21] Z. J. Liu, Y.K. Guo, J.G. Bai, “Exogenous hydrogen peroxide changes antioxidant enzyme activity and protects ultrastructure in leaves of two cucumber ecotypes under osmotic stress”, Journal of Plant Growth Regulation, vol. 29, pp. 171-183, 2010.
[22] H.K. Lichtenthaler, F. Babani, “Detection of photosynthetic activity and water stress by imaging the red chlorophyll fluorescence”,
Plant Physiology and Biochemistry, vol. 38, pp. 889-895, 2000.
[23] C. Lu, J. Zhang, “Effects of water stress on photosystem II photochemistry and its thermostability in wheat plants”, Journal of Experimental Botany, vol. 50, pp. 1199- 1206, 1999.
[24] A. Wingler, W.P. Quick, R.A. Bungard, et al., “The role of photorespiration during drought stress: an analysis utilizing barley mutants with reduced activities of photo respiratory enzymes”, Plant, Cell and Environment, vol. 22, pp. 361-373, 1999.
[25] K. Maxwell, G.N. Johnson, “Chlorophyll fluorescence-a practical guide”, Journal of Experimental Botany, 51(345), pp. 659-668, 2000.
[26] S. Hazrati, Z. Tahmasebi-Sarvestani, S.A.M. Modarres-Sanavy, et al., “Effects of water stress and light intensity on chlorophyll fluorescence parameters and pigments of Aloe vera L.”, Plant Physiology and Biochemistry, vol. 106, pp. 141-148, 2016.
[27] S.K. Singh, R.K. Raja, “Regulation of photosynthesis, fluorescence, stomatal conductance and water-use efficiency of cowpea (Vigna unguiculata [L.] Walp.) under drought”, Journal of Photochemistry and Photobiology B: Biology vol. 105, issue 1, pp. 40-50, 2011.