Kadri GÖKÇE, Ramazan SABIRLI, Aylin KÖSELER, İbrahim TÜRKÇÜER, Atakan YILMAZ

Koroner arter hastalığı olan hastalarda matriks metalloproteinaz-2 ve anjiyotensin-1 gen polimorfizmleri

Giriş: MMP'lerin lokal fibröz kapak üzerindeki etkisi aterosklerotik plakların kopmasına yol açar ve sonuç olarak kronik bir hastalığı akut miyokard enfarktüsüne çevirerek ani ölüme sebep olabilir. ACE (Anjiotensin dönüştürücü enzim)'nin çeşitli biyolojik eylemleri iskemik kalp hastalığının patogenezinde yer alır. Bu nedenle biz de çalışmamızda anjiotensin converting enzim (ACE) ve matrix metalloproteinaz 2 (MMP-2) gen polimorfizminin KAH gelişimindeki rolünü değerlendirmeyi amaçladık.Gereç ve yöntem: Çalışmaya toplamda 300 gönüllü(100 sağlıklı/200 hasta) dahil edildi. ACE gen I/D için PCR-RFLP yöntemi ve MMP-2 (-1306 C/T) polimorfizmi için DNA dizi analizi yöntemi uygulandı. Bulgular: Gruplar arasında ACE-1 genotiplerine bakıldığında CAD grubunda D/D genotip frekansı %50, I/D genotip frekansı %29, I/I genotip frekansı %21; kontrol grubunda D/D genotip frekansı %37, I/D genotip frekansı %45, I/I genotip frekansı %18 olarak saptanmıştır. Gruplar arasında ACE-1 geni genotipi açısından anlamlı fark mevcuttur (p=0,021). CAD grubunda ise D/D genotip frekansı kontrol grubuna göre daha yüksektir. ACE-1 geni alleli sayılarına bakıldığında her iki gruptaki I ve D allel frekansları benzerdir ve istatistiksel olarak anlanmlı fark mevcut değildir (p=0,314). Sonuç: Bu çalışma MMP-2 (rs243865) gen polimoforfizmi ile koroner arter hastalığı arasında bir ilişki olmadığını göstermiştir. I/D genotip frekansı kontrol grubunda KAH grubuna göre daha yüksek, D/D genotip frekansı ise KAH grubunda kontrol grubuna göre daha yüksekti. D/D genotipli hastalarda KAH hastalığı riski artmıştır.

Anahtar Kelimeler:

ACE-1 gen polimorfizmi, MMP-2 (rs243865) gen polimorfizmi, Türk populasyonu, Koroner arter hastalığı

Matrix metalloproteinase-2 and angiotensin-1 gene polymorphisms in patient who have coronary artery disease

Introduction: The rupture of atherosclerotic plaques is caused by the impact of matrix metalloproteinases (MMPs) upon the local fibrous valve and so might convert a chronic disease to a myocardial infarction, ultimately leading to instant death. Angiotensin converting enzyme (ACE) is actively engaged in the pathogenesis of ischemic heart disease. This study tries to unravel whether/how ACE and MMP-2 gene polymorphism contributes to the occurrence of Coronary Artery Disease (CAD). Materials and methods: A total of 300 individuals (100 healthy/200 patients) were included in the study. A PCR-RFLP method was utilized for ACE gene I/D and DNA sequencing MMP-2 (-1306 C/T) polymorphisms. Results: The ACE-1 gene D/D, I/D and I/I genotype frequency of the CAD cohort was 50%, 29%, and 21%, respectively whereas that of the healthy control cohort was 37%, 45% and 18%, respectively. Our findings indicate that the groups differed significantly in relation to the ACE-1 genotypes (p=0.021). The frequencies of ACE-1 gene allele I and D in both cohorts did not reveal a significant difference (p=0.314). In addition, the two groups did not manifest MMP-2 (rs243865) gene polymorphism. Conclusion: The association between MMP-2 gene polymorphism and CAD is too weak to suggest a conclusive evidence. The I/D genotype frequency remained higher in the healthy individuals than in the CAD cohort, while in the CAD group D/D genotype was more frequently than control group. Finally, the patients with D/D genotype tend to bear greater risk for cardiovascular diseases.

Keywords:

MMP-2 gene polymorphism, ACE-1 gene polymorphism, coronary artery disease, Turkish population,

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