Firdevs KAHVECİOĞLU, Mutlu ÖZCAN, Hayriye Esra ÜLKER, Gül TOSUN

500-1450 ppm Sodyum Florür ve Amin Florür Bazlı, Farklı Deterjan İçerikli Pediatrik Diş Macunlarının Oksidatif Stres ve Hücre Canlılığı Üzerine Etkisi

Amaç: Bu çalışmada, sodyum florür ve amin florür içeren pediatrik diş macunlarının farklı deterjan içeriklerinin oksidatif stres ve hücre canlılığı üzerine etkisi değerlendirilmiştir. Gereç ve Yöntemler: Sodyum florür (Sensodyne Pronamel-SP; Ipana Kids-IK; Signal Kids-SK; Oral B-OB) ve amin florür (Elmex-EL; Elmex Junior-EJ) içeren pediatrik diş macunları kullanıldı. SP, IK, EL kokamidopropil betain deterjan içerirken, SK, OB sodyum lauril sülfat ve EJ olaflur deterjan içerikliydi. Diş macunu numuneleri önceden belirlenmiş farklı konsantrasyonlarda medyum ile seyreltildi ve L929 fibroblast hücreleri, diş macunu ekstraktlarına 37 °C’de 2 dakika maruz bırakıldı.Hücre canlılığı metil tetrazolyum testi kullanılarak test edilirken, reaktif oksijen türlerinin oluşumu (ROS) flow sitometrisi kullanılarak tespit edildi. Bulgular: 1:1’den 1:32’ye azalan konsantrasyon oranı, EL haricinde sitotoksisiteyi azalttı (p

Effect of Pediatric Toothpastes Based on 500 to 1450 ppm Sodium Fluoride and Amine Fluoride with Different Detergents on Oxidative Stress and Cell Viability

Objective: This study evaluated the effect of sodium fluoride and amine fluoride pediatric toothpastes with different detergents on oxidative stress and cell viability. Materials and Methods: Pediatric toothpastes containing sodium fluoride (Sensodyne Pronamel-SP; Ipana Kids-IK; Signal Kids-SK; Oral B-OB) and amine fluoride (Elmex-EL; Elmex Junior-EJ) were obtained. While SP, IK, EL contained cocamidopropyl betaine detergent, SK, OB sodium lauryl sulfate and EJ had olaflur detergents. Toothpaste samples were diluted with medium at different predetermined concentrations. L929 fibroblast cells were exposed to toothpaste extracts at 37 °C for 2 min. Cell viability was tested using methyl tetrazolium test, while the formation of reactive oxygen species (ROS) was detected using flow cytometry. Results: The decreasing concentration ratio from 1:1 to 1:32 decreased the cytotoxicity (p

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