Melatonin Uygulamasının Kronik Kadmiyum Toksikasyonuna Maruz Kalan Sıçanlarda Bazı Serum İmmun-Regülatör Sitokinler Üzerine Etkisi

Bu çalışmada kronik kadmiyum toksikasyonuna maruz kalan sıçanlarda melatonin’in bazı serum immun-regülatör sitokin seviyeleri üzerine etkilerinin araştırılması amaçlanmıştır. Bu amaç doğrultusunda, hayvanlar (n =32); kontrol grubu (K) kadmiyum grubu (Cd), melatonin grubu (Mlt) ve kadmiyum + melatonin grubu (CdMlt) olmak üzere rastgele dört eşit gruba ayrıldı. Cd ve CdMlt gruplarındaki hayvanlara, 4 hafta boyunca haftada üç kez gastrik gavaj yoluyla oral kadmiyum klorür (CdCl2) (2 mg/kg/gün) verildi. Öte yandan Mlt ve CdMlt gruplarına 4 hafta boyunca haftada beş kez oral Mlt (100 mg/ kg/gün) uygulaması yapıldı. Kontrol grubunda yer alan sıçanlara herhangi bir uygulama yapılmadı. Deney periyodundan sonra, sıçanlar sakrifiye edildi ve kan örnekleri antikoagulant içermeyen tüplere alındı. Deneme sonunda serum IL-1β, IL-2, IL-6, TNF-α ve INFγ konsantrasyonları belirlendi. IL-1β, IL-2 ve IL-6 seviyelerinde deney grupları arasında bir değişiklik bulunmadı (p >0,05). Ayrıca, Mlt uygulaması CdMlt grubundaki TNF-α düzeylerini Cd grubuna kıyasla iyileştirdi (p <0,05). IFNγ seviyeleri Cd grubu ile karşılaştırıldığında en yüksek oranda C ve Mlt gruplarında tespit edildi (p<0,05). Sonuç olarak, Mlt uygulaması Cd toksisitesine maruz kalan sıçanlarda sadece TNF-α düzeylerinde önemli bir değişikliğe neden olmuştur.

Anahtar Kelimeler:

Kadmiyum, melatonin, serum, sitokin, sıçan

The Effects Of Melatonin Treatment On Some Serum Immunoregulatory Cytokine Levels In Rats Exposed To Chronic Cadmium Toxicity

The present study aimed to investigate the effect of melatonin treatment on some serum immunoregulatory cytokine levels in rats exposed to chronic cadmium toxicity. For this purpose, animals (n = 32) were divided randomly into four equal groups as untreated control (C), cadmium (Cd), melatonin (Mlt) and Cd + Mlt (CdMlt). The rats in Cd and CdMlt groups received cadmium chloride (CdCl2) (2 mg/kg/day) orally by gastric gavage three times a week for 4 weeks. On the other hand, Mlt (100 mg/kg/day) was orally administrated to Mlt and CdMlt groups five times a week for 4 weeks. C group was not received any treatment. After the treatments, the animals were sacrificed and blood samples were taken to without anticoagulant tubes. Then, levels of IL-1β, IL-2, IL- 6, TNF-α, and INFγ in the serum were determined. It was not found any change among the groups according to IL-1β, IL-2, and IL-6 levels (p>0,05). Besides, the administration of Mlt ameliorated the TNF-α levels in CdMlt group compared to Cd (p<0,05). IFNγ levels were found the highest in C and Mlt groups compared to Cd (p<0,05). In conclusion, Mlt treatment caused a significant change only in TNF-α levels in rats exposed to Cd.

Keywords:

Cadmium, cytokine, melatonin, serum, rat,

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