Nail Tekin ÖNDER, Taygun GÖKDEMİR, Muhammet Can KILIÇ, Savaş YILDIZ, Yavuz ÖZTÜRKLER

Insülin Lispro’nun Kriyoprezervasyon Sırasında Koç Sperması Üzerine Etkileri

Sunulan çalışmada, insülin ilave edilen sperma sulandırıcısının koç sperması üzerindeki etkilerininin çözdürme sonrası aşamada değerlendirilmesi amaçlanmıştır. Tuj ırkı 5 adet koçtan beş kez elektro-ejakülatör ile sperma alındı. Deneme grupları, kontrol grubu, 15 IU ve 20 IU insulin lispro ilave edilen sperma sulandırıcıları ile oluşturuldu. Çözdürme sonrası, motilite ve plazma membran bütünlüğü mikroskopi ile değerlendirildi. Akrozom bütünlüğü (Fitc-Peanut Agglutinin) ve mitokondrial membran potansiyeli (Rhodamine 123) akış sitometrisi kullanılarak, propidium iodide (PI) ile ikili boyama yapılarak değerlendirildi. Motilite ve plazma membran fonksiyonel bütünlüğünün kontrol grubuna göre daha iyi korunduğu bulundu (p<0.05). Akrozom bütünlüğü sonuçları kontrol ve 15 IU insülin grubu arasında benzer olduğu (p>0.05), ancak 20 IU insulin grubunda akrozom bütünlüğünün olumsuz etkilediği bulundu (p<0.05). Mitokondrial membran potansiyeli, 20 IU insulin içeren grupta daha yüksek bulundu (p<0.05). Sonuç olarak, insulin ilave edilmiş sulandırıcılar ile dondurulan koç spermasında enerji metabolizmasının uyarıldığı ve spermanın kontrol grubuna göre daha iyi korunduğu düşünülmüştür.

Anahtar Kelimeler:

Tuj, Koç sperması, İnsülin, Kriyoprezervasyon

Effects of Insulin Lispro on Ram Semen Cryopreservation

In the present study, it was aimed to evaluate the effects of semen extender with added insulin on ram semen at the post-thaw stage. Semen was collected from 5 Tuj rams five times with an electro-ejaculator. Experimental groups were designated as control group and groups containing 15 IU and 20 IU insulin lispro added semen extenders. At the post-thaw stage, motility and plasma membrane integrity were evaluated by microscopy. Acrosome integrity (Fitc-Peanut Agglutinin) and mitochondrial membrane potential (Rhodamine 123) were assessed by dual staining with propidium iodide (PI) using flow cytometry. It was found that motility and plasma membrane functional integrity were better preserved in the control group (p<0.05). Acrosome integrity results were similar between the control and 15 IU insulin groups (p>0.05), but acrosome integrity was negatively affected in the 20 IU insulin group (p<0.05). Mitochondrial membrane potential was higher in the group containing 20 IU insulin (p<0.05). As a result, it was thought that energy metabolism was stimulated in ram semen frozen with insulin-added extenders, and the semen was better preserved than the control group.

Keywords:

Tuj, Ram semen, Insulin, Cryopreservation,

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