The mRNA expression pattern of skeletal muscle regulatory factors in divergent phenotype swine breeds

Kas düzenleyici faktörler (KDF), kas büyümesi ve gelişmesinde önemli bir rol oynar. Bu nedenle domuzlarda et üretim özellikleri için aday genler olarak kabul edilirler. Bu temel helix-loop-heliks (tHLH) ve eşleştirilmiş kutu transkripsiyon faktörleri ile kemik morfogenetik proteinleri miyogenesisi düzenlerler: kas liflerinin oluşumu başlatmak ve kas spesifik genlerin transkripsiyonunu düzenlerler. Bu çalışmanın amacı, Duroc (yüksek et yağ oranı) ve Pietrain (düşük et yağ oranı) domuz ırklarında yetişkin iskelet kası (longissimus dorsi) transkripsiyon faktörü (TF) genlerinin (MYOD, MYF5, MYOG, PAX3, PAX7, BMP2 ve BMP4) mRNA ifadesini araştırmaktır. Bu amaçla, safkan Duroc ve Pietrain hayvanlar (her ırkan cins n = 5) mRNA ifade çalışması için kullanılmıştır. En yüksek gen ifadesi MYOG için, ve onu takiben BMP2 için gözlendi. En düşük gen ifadesi PAX3 için gözlendi. Ayrıca bu genlerin ifadeleri Duroc ve Pietrain ırklarında istatistiksel olarakta araştırılmıştır. Yedi gen içinde MYF5 ve PAX3 genleri Duroc ve Pietrain ırkları arasında (P

Anahtar Kelimeler:

Duroc, Pietrain, gen ifadesi, genler, elçi RNA, fenotip, domuz, iskelet kası, transkripsiyon faktörleri

İskelet kası düzenleyici faktörlerin mRNA ifadesinin farklı özellikteki domuzlarda gösterimi

Muscle regulatory factors (MRFs) play a major role in muscle growth and development. Thus, they are considered as candidate genes for meat production traits in pigs. These basic helix-loop-helix (bHLH) and paired box transcription factors together with Bone morphogenetic proteins, regulate myogenesis: they initiate the formation of muscle fibers and regulate the transcription of muscle specific genes. The objective of this study was to investigate the mRNA abundance of transcription factor (TF) genes (MYOD, MYF5, MYOG, PAX3, PAX7, BMP2 and BMP4) in the adult skeletal muscle (longissimus dorsi) of divergent Duroc (high meat fat ratio) and Pietrain (low meat fat ratio) pig breeds. For this purpose, purebred Duroc and Pietrain animals (for each breed n = 5) were used for mRNA expression study. Highest gene expression was observed for MYOG, followed by BMP2. The lowest gene expression was observed for PAX3. Moreover, we statistically investigated differences in the expression profiles of these genes between the Duroc and Pietrain breeds. Among seven genes MYF5 and PAX3 showed different expression (P<0.01) between Duroc and Pietrain breeds. Duroc showed higher MYF5 and PAX3 mRNA abundance compared to the Pietrain breed. Although PAX3 gene was the lowest expressed, results suggest that Duroc breed may have more skeletal muscle regeneration potential compared to Pietrain breed.

Keywords:

Duroc, Pietrain, gene expression, genes, messenger RNA, phenotypes, pigs, skeletal muscle, transcription factors,

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