MEHMET ULAŞ ÇINAR, Huitao FAN

The mRNA expression pattern of skeletal muscle regulatory factors in divergent phenotype swine breeds

Kas düzenleyici faktörler (KDF), kas büyümesi ve gelişmesinde önemli bir rol oynar. Bu nedenle domuzlarda et üretim özellikleri için aday genler olarak kabul edilirler. Bu temel helix-loop-heliks (tHLH) ve eşleştirilmiş kutu transkripsiyon faktörleri ile kemik morfogenetik proteinleri miyogenesisi düzenlerler: kas liflerinin oluşumu başlatmak ve kas spesifik genlerin transkripsiyonunu düzenlerler. Bu çalışmanın amacı, Duroc (yüksek et yağ oranı) ve Pietrain (düşük et yağ oranı) domuz ırklarında yetişkin iskelet kası (longissimus dorsi) transkripsiyon faktörü (TF) genlerinin (MYOD, MYF5, MYOG, PAX3, PAX7, BMP2 ve BMP4) mRNA ifadesini araştırmaktır. Bu amaçla, safkan Duroc ve Pietrain hayvanlar (her ırkan cins n = 5) mRNA ifade çalışması için kullanılmıştır. En yüksek gen ifadesi MYOG için, ve onu takiben BMP2 için gözlendi. En düşük gen ifadesi PAX3 için gözlendi. Ayrıca bu genlerin ifadeleri Duroc ve Pietrain ırklarında istatistiksel olarakta araştırılmıştır. Yedi gen içinde MYF5 ve PAX3 genleri Duroc ve Pietrain ırkları arasında (P

Anahtar Kelimeler:

Duroc, Pietrain, gen ifadesi, genler, elçi RNA, fenotip, domuz, iskelet kası, transkripsiyon faktörleri

İskelet kası düzenleyici faktörlerin mRNA ifadesinin farklı özellikteki domuzlarda gösterimi

Muscle regulatory factors (MRFs) play a major role in muscle growth and development. Thus, they are considered as candidate genes for meat production traits in pigs. These basic helix-loop-helix (bHLH) and paired box transcription factors together with Bone morphogenetic proteins, regulate myogenesis: they initiate the formation of muscle fibers and regulate the transcription of muscle specific genes. The objective of this study was to investigate the mRNA abundance of transcription factor (TF) genes (MYOD, MYF5, MYOG, PAX3, PAX7, BMP2 and BMP4) in the adult skeletal muscle (longissimus dorsi) of divergent Duroc (high meat fat ratio) and Pietrain (low meat fat ratio) pig breeds. For this purpose, purebred Duroc and Pietrain animals (for each breed n = 5) were used for mRNA expression study. Highest gene expression was observed for MYOG, followed by BMP2. The lowest gene expression was observed for PAX3. Moreover, we statistically investigated differences in the expression profiles of these genes between the Duroc and Pietrain breeds. Among seven genes MYF5 and PAX3 showed different expression (P<0.01) between Duroc and Pietrain breeds. Duroc showed higher MYF5 and PAX3 mRNA abundance compared to the Pietrain breed. Although PAX3 gene was the lowest expressed, results suggest that Duroc breed may have more skeletal muscle regeneration potential compared to Pietrain breed.

Keywords:

Duroc, Pietrain, gene expression, genes, messenger RNA, phenotypes, pigs, skeletal muscle, transcription factors,

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1. Rehfeldt C, Pas MFWT, Wimmers K, Brameld JM, Nissen PM, Berri C, Valente LMP, Power DM, Picard B, Stickland NC: Advances in research on the prenatal development of skeletal muscle in animals in relation to the quality of muscle-based food. I. Regulation of myogenesis and environmental impact. Animal, 5 (5): 703-717, 2011.
2. Friedrichs M, Wirsdoerfer F, Flohe SB, Schneider S, Wuelling M, Vortkamp A: Bmp signaling balances proliferation and differentiation of muscle satellite cell descendants. BMC Cell Biol, 12, 26, 2011.
3. Braun T, Gautel M: Transcriptional mechanisms regulating skeletal muscle differentiation, growth and homeostasis. Nat Rev Mol Cell Biol, 12 (6): 349-361, 2011.
4. Martin PT: Role of transcription factors in skeletal muscle and the potential for pharmacological manipulation. Curr Opin Pharmacol, 3 (3): 300-308, 2003.
5. Rehfeldt C, Pas MFWT, Wimmers K, Brameld JM, Nissen PM, Berri C, Valente LMP, Power DM, Picard B, Stickland NC: Advances in research on the prenatal development of skeletal muscle in animals in relation to the quality of muscle-based food. II - Genetic factors related to animal performance and advances in methodology. Animal, 5 (5): 718-730, 2011.
6. Chen D, Zhao M, Mundy GR: Bone morphogenetic proteins. Growth Factors, 22 (4): 233-241, 2004.
7. Lee JY, Musgrave D, Pelinkovic D, Fukushima K, Cummins J, Usas A, Robbins P, Fu FH, Huard J: Effect of bone morphogenetic protein-2 expressing muscle derived cells on healing of critical sized bone defects in mice. J Bone Joint Surg Am, 83A (7): 1032-1039, 2001.
8. Christiansen JH, Goles EG, Wilkinson DG: Molecular control of neural crest formation, migration and differentiation. Curr Opin Cell Biol, 12 (6): 719-724, 2000.
9. Katagiri T, Komaki M, Namiki M, Inada M, Yamaguchi A, Wozney JM, Rosen V, Suda T: Inactivation of the Myo-d family is required for the Bmp-2 induced osteoblast differentiation of muscle cells. J Bone Miner Res, 10, S148-S148, 1995.
10. Allen DL, Greybeck BJ, Hanson AM, Cleary AS, Lindsay SF: Skeletal muscle expression of bone morphogenetic protein-1 and tolloid-like-1 extracellular proteases in different fiber types and in response to unloading, food deprivation and differentiation. J Physiol Sci, 60 (6): 447-447, 2010.
11. Rehfeldt C, Fiedler I, Dietl G, Ender K: Myogenesis and postnatal skeletal muscle cell growth as influenced by selection. Livest Prod Sci, 66 (2): 177-188, 2000.
12. Murani E, Muraniova M, Ponsuksili S, Schellander K, Wimmers K: Identification of genes differentially expressed during prenatal develop-ment of skeletal muscle in two pig breeds differing in muscularity. BMC Dev Biol, 7, 109, 2007.
13. Bi P, Kuang S: Stem cell niche and postnatal muscle growth. J Anim Sci, 90 (3): 924-935, 2012.
14. Moss FP, Leblond CP: Satellite cells as the source of nuclei in muscles of growing rats. Anat Rec, 170 (4): 421-435, 1971.
15. Psilander N, Damsgaard R, Pilegaard H: Resistance exercise alters Mrf and Igf-I mRNA content in human skeletal muscle. J Appl Physiol, 95 (3): 1038-1044, 2003.
16. Fan H, Cinar MU, Phatsara C, Tesfaye D, Tholen E, Looft C, Schellander K: Molecular mechanism underlying the differential Myf6 expression in postnatal skeletal muscle of Duroc and Pietrain breeds. Gene, 486 (1-2): 8-14, 2011.
17. Gil M, Delday MI, Gispert M, MF IF, Maltin CM, Plastow GS, Klont R, Sosnicki AA, Carrion D: Relationships between biochemical characteristics and meat quality of Longissimus thoracis and Semi-membranosus muscles in five porcine lines. Meat Sci, 80 (3): 927-933, 2008.
18. Lefaucheur L, Milan D, Ecolan P, Le Callennec C: Myosin heavy chain composition of different skeletal muscles in Large White and Meishan pigs. J Anim Sci, 82 (7): 1931-1941, 2004.
19. Rehfeldt C, Henning M, Fiedler I: Consequences of pig domestication for skeletal muscle growth and cellularity. Livest Sci, 116 (1-3): 30-41, 2008.
20. ZDS: Richtlinie fuer die Stationspruefung auf Mastleistung, Schlachtkoerperwert und Fleischbeschaffenheit beim Schwein. Zentralverband der Deutschen Schweineproduktion eV, Ausschussfuer Leistungspruefung und Zuchtwertschaetzung, Bonn, 2003.
21. Rozen S, Skaletsky H: Primer3 on the www for general users and for biologist programmers. Methods Mol Biol, 132, 365-386, 2000.
22. Hammer Ø, Harper DAT: Paleontological data analysis. 1st ed., 368p, Blackwell Publishing Ltd, Oxford, 2006.
23. Cagnazzo M, te Pas MF, Priem J, de Wit AA, Pool MH, Davoli R, Russo V: Comparison of prenatal muscle tissue expression profiles of two pig breeds differing in muscle characteristics. J Anim Sci, 84 (1): 1-10, 2006.
24. Ringner M: What is principal component analysis? Nat Biotechnol, 26 (3): 303-304, 2008.
25. Hawke TJ, Garry DJ: Myogenic satellite cells: physiology to molecular biology. J Appl Physiol, 91 (2): 534-551, 2001.
26. Siegel AL, Kuhlmann PK, Cornelison DD: Muscle satellite cell pro- liferation and association: New insights from myofiber time-lapse imaging. Skelet Muscle, 1 (1): 7, 2011.
27. Seale P, Sabourin LA, Girgis-Gabardo A, Mansouri A, Gruss P, Rudnicki MA: Pax7 is required for the specification of myogenic satellite cells. Cell, 102 (6): 777-786, 2000.
28. Maroto M, Reshef R, Munsterberg AE, Koester S, Goulding M, Lassar AB: Ectopic Pax-3 activates MyoD and Myf-5 expression in embryonic mesoderm and neural tissue. Cell, 89 (1): 139-148, 1997.
29. Edwards DB, Tempelman RJ, Bates RO: Evaluation of Duroc- vs. Pietrain-sired pigs for growth and composition. J Anim Sci, 84 (2): 266-275, 2006.
30. Edwards DB, Bates RO, Osburn WN: Evaluation of Duroc- vs. Pietrain-sired pigs for carcass and meat quality measures. J Anim Sci, 81 (8): 1895-1899, 2003.
31. Wang YX, Rudnicki MA: Satellite cells, the engines of muscle repair. Nat Rev Mol Cell Bio, 13, 127-133, 2012.
32. Gayraud-Morel B, Chretien F, Flamant P, Gomes D, Zammit PS, Tajbakhsh S: A role for the myogenic determination gene Myf5 in adult regenerative myogenesis. Dev Biol, 312 (1): 13-28, 2007.
33. Ustanina S, Carvajal J, Rigby P, Braun T: The myogenic factor Myf5 supports efficient skeletal muscle regeneration by enabling transient myoblast amplification. Stem Cells, 25 (8): 2006-2016, 2007.
34. Pierzchała M, Pareek CS, Lisowski P, Urbanski P, Goluch D, Czarnik U, Kamyczek M, Rozycki M, Cooper RG, Kuryl J: Expression profile of Myf5 and Myf6 genes in skeletal muscles of young growing gilts of five breeds at different ages, based on the most stable reference genes. Anim Sci Pap Rep, 29 (3): 231- 246, 2011.
35. Caliaro F, Maccatrozzo L, Toniolo L, Reggiani C, Mascarello F, Patruno M: Myogenic regulatory factors expressed during postnatal hyper- plastic growth in porcine muscles. Basic Appl Myol, 15 (2): 55-60, 2005.
36. Carvajal JJ, Cox D, Summerbell D, Rigby PWJ: Control of the expression of the Mrf4 and Myf5 genes: A BAC transgenic approach. Int J Dev Biol, 45, S139-S140, 2001.
37. Cinar MU, Fan H, Neuhoff C, Große-Brinkhaus C: Eqtl analysis and association of Myf6 mrna expression with meat quality traits in pigs. Kafkas Univ Vet Fak Derg, 18 (2): 235-242, 2012.
38. te Pas MFW, Harders FL, Soumillion A, Born L, Buist W, Meuwissen THE: Genetic variation at the porcine Myf-5 gene locus. Lack of association with meat production traits. Mamm Genom, 10 (2): 123-127, 1999.
39. Zhang RF, Chen H, Lei CZ, Zhang CL, Lan XY, Zhang YD, Zhang HJ, Bao B, Niu H, Wang XZ: Association between polymorphisms of Mstn and Myf5 genes and growth traits in three Chinese cattle breeds. Asian Austral J Anim, 20 (12): 1798-1804, 2007.