Mehmet HALIGÜR, ÖZLEM ÖZMEN, Sevil Atalay VURAL, Şenay BERKIN

Pathological, ımmunohistochemical and electron microscopical examinations on chorioallantoic membrane lesions in experimental fowl poxvirus ınfection

Bu çalışmada, tavuk çiçek virusu ile deneysel olarak enfekte edilen korioallantoik membranda immunohistokimyasal yöntemlerle viral antijenin varlığı ve elektron mikroskobik olarak da virus ile inkluzyon cisimciklerinin gelişim aşamalarının incelenmesi amaçlanmıştır. İngiltere Weybirdge Veteriner Merkez Laboratuvarından getirilen liyofilize tavuk çiçek virus 92 suşu 103 titrede kullanıldı. Deneysel olarak bu suş ile 10 günlük 30 adet SPF embriyolu tavuk yumurtasının korioallantoik membranına ekim yapıldı. Suşun inokulasyonundan 7 gün sonra membranda grimsi-beyaz, 0.5-2.5 mm arasında değişen çapta çiçek lezyonları görüldü. Bu lezyonlar makroskobik ve mikroskobik olarak değerlendirildi. Histopatolojik incelemede hipertrofik ve hiperplastik epitel hücrelerinde intrasitoplazmik inkluzyon cisimcikleri ile balonumsu dejenerasyona rastlandı. Indirekt immunoperoksidaz tekniği ile yapılan boyamalarda intrasitoplazmik inkluzyon cisimciklerinin yanı sıra proliferatif epitel hücrelerinin sitoplazmalarında da sadece elektron mikroskobik olarak görülebilen histopatolojik incelelemerde saptanmayan makriks inklüzyonlarının varlığını kanıtlayan pozitif immunoreaksiyonlar görüldü. Elektron mikroskobik incelemede virusun gelişim aşamalarından oluşan çok sayıda matriks inkluzyon cisimcikleri saptandı. Sonuç olarak, bu çalışma immunoperoksidaz tekniğinin tavuk çiçek virus (FVP) enfeksiyonda erken devrelerin morfolojik teşhisinde kullanılabileceğini göstermiştir.

Anahtar Kelimeler:

çiçek hastalığı virüsü, hayvan patolojisi, koryoallantoyik zar, elektron mikroskopisi, epitel, deneysel enfeksiyon, dölüt, kanatlı çiçek virüsü, tavuk, immünohistokimya

Deneysel tavuk çiçek virus enfeksiyonunda korioallantoik membrandaki patolojik, ımmunohistokimyasal ve elektron mikroskobik incelemeler

The objectives of this study are immunohistochemical detection of the viral antigen and structure of fowl pox virus and developmental stage of inclusion bodies by electron microscopy in experimentally infected chorioallantoic membrane (CAM). Lyophilized fowl pox virus, strain 92 which was obtained from the Veterinary Central Laboratory in Weybirdge, (UK) used in 103 titer. Thirty, 10 days old Specific Pathogen Free (SPF) chicken embryonated eggs chorioallantoic membrane that experimentally infected with this strain. Seven days after inoculation of strain, pocks lesions occurred as 0.5-2.5mm diameter in size with grayish-white color and evaluated macroscopically and microscopically. At the histopathological examination, intracytoplasmic inclusion bodies and ballooning degeneration were detected in the hyperplastic and hypertrophic epithelial cells. Positive immunoreactions were also seen in the cytoplasm of the proliferative epithelial cells which may be reflected matrix inclusion bodies that is early form of inclusion bodies and not seen in histopathologically, but seen in ultrastructural examination. Numerous matrix inclusions which consist of developmental stage of virus were detected in effected cells by electron microscopic examination. As a result, this study indicated that, immunoperoxidase technique may be use in the morphologic diagnosis of early stage of the fowl poxvirus (FVP) infection.

Keywords:

Avipoxvirus, animal pathology, chorioallantoic membrane, electron microscopy, epithelium, experimental infection, fetus, fowl pox virus, fowls, immunohistochemistry,

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