ARZU FINDIK, Nergiz AKAN, ERTAN EMEK ONUK, Duygu ÇAKIROĞLU, ALPER ÇİFTCİ

Methicilin resistance profile and molecular typing of Staphylococcus aureus strains isolated from noses of the healthy dogs

Bu çalışma, Samsun İli’ndeki sağlıklı köpeklerin burunlarından izole edilen Staphylococcus aureus suşlarının metisilin direnç profilini belirlemek ve bu izolatların koagulaz (coa) ve protein A (spa) gen polimorfizmlerini araştırmak üzere gerçekleştirildi. Toplam 80 izolat, fenotipik ve genotipik olarak koagulaz pozitif S. aureus olarak identifiye edildi. İzolatların tümü agar disk difüzyon yöntemi ile metisilin duyarlı bulundu. Bununla birlikte, mecA ve fem genlerini belirlemeye yönelik yapılan multipleks PCR’da 3 (%3.75) izolatın mecA genine sahip olduğu bulunurken, suşların hiçbiri fem pozitif değildi. Seksen S. aureus izolatından coa ve spa genlerinin amplifikasyonu sonucunda sırasıyla 9 ve 3 farklı PCR ürünü elde edildi. Multipleks PCR ile metisilin dirençli S. aureus olarak identifiye edilen 3 izolatın da aynı koagulaz ve spa grubunda olduğu belirlendi. Çalışılan bölgede, çok sayıda coa genotipine ve birden fazla spa grubuna sahip S. aureus suşlarının bulunduğu ve MRSA izolatlarının tek bir coa ve spa grubuna dahil olduğu sonucu çıkarıldı. Bu sonuçlar sağlıklı köpeklerde MRSA kolonizasyon oranının yüksek olduğunu ve insan sağlığı için risk teşkil edebileceğini gösterdi.

Anahtar Kelimeler:

genetik polimorfizm, zoonoz, burun, metisilin, genler, ilaç direnci, köpek, amplifikasyon, Staphylococcus aureus

Sağlıklı köpeklerin burunlarından izole edilen Staphylococcus aureus suşlarının metisilin direnç profili ve moleküler tiplendirilmesi

This study was conducted to determine the profile of methicillin resistance of Staphylococcus aureus strains isolated from noses of healthy dogs in Samsun and to investigate the coagulase (coa) and protein A (spa) gene polymorphisms of these isolates. A total of 80 isolates were identified as coagulase positive S. aureus phenotypically and genotypically. All of the isolates were found to be methicillin sensitive determined by agar disc diffusion method. However, in multiplex PCR performed to detect mecA and fem genes, 3 (3.75%) isolates were found to have mecA gene and none were fem positive. Amplification of the coa and spa genes from 80 S. aureus isolates produced 9 and 3 different PCR products, respectively. All 3 methicillin resistant isolates identified by multiplex PCR were observed in the same coa and spa groups. It was concluded that various coagulase types and more than one spa types of S. aureus are present in the region where the study was performed and MRSA isolates were included in unique coa and spa groups. Also, the MRSA colonization rate was considered to be high and may pose a risk for human health.

Keywords:

genetic polymorphism, zoonoses, nose, methicillin, genes, drug resistance, dogs, amplification, Staphylococcus aureus,

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