ÖZLEM ÖNEN ÇELEBİ, Burak GÖKÇE, Gürsel ERGEN, Sema ÜÇÜNCÜ İŞİSAĞ

Ham petrolün Poecilia sphenops (Valenciennes, 1846) (Cyprinidae, Teleostei) deri histolojisi üzerine etkileri

Günümüzde endüstriyel teknolojinin gelişmesinde temel enerji kaynağı olan ham petrol, ekotoksikant olarak geniş çapta araştırılmaktadır. Ham petrolün teleostlarda deri üzerine etkilerini ortaya koymak için Poecilia sphenops örnekleri, ham petrolün suda çözünebilen kısımlarına dört farklı etki süresince (24, 48, 72, 96 saat) üç ayrı konsantrasyonuna (%10, %20, %40) maruz bırakılmış ve histolojik değişiklikler belirtilmiştir. Düşük konsantrasyon, kısa uygulama süresinde epidermiste hafif aşınma ve dermiste melanofor birikimi gözlenmiştir. Beklenildiği üzere dermis bozulması, subkutanöz kas tabakasında nekrotik alanların oluşumu ve fibrozis gibi ham petrolün çarpıcı etkileri, artan uygulama süresi ve konsantrasyona paralellik göstermiştir.

Anahtar Kelimeler:

Poecilia, Poeciliidae, maruz kalma, fibroz, histopatoloji, doku ölümü, yağlar, deri, deri lezyonları, toksisite, toksikoloji, su kirliliği

The effects of crude oil on skin histology of Poecilia sphenops (Valenciennes, 1846) (Cyprinidae, Teleostei)

As being the main energy source that is needed for all of the industrial processes, crude oil is evaluated as a widely investigated ecotoxicant. In order to identify the effects of crude oil on the skin of teleosts, Poecilia sphenops specimens were exposed to three different concentration of soluble part of crude oil (10%, 20%, 40%) at four different exposure time (24, 48, 72, 96 h); and the histopathological changes were reported. A slight epidermis corrosion and melanophore aggregation in dermis were observed at low concentration and short exposure time. As estimated, the striking effects of the crude oil; such as dermis disintegration, necrotic areas and fibrosis in subcutaneous muscle layer; were extended prominently in parallel with increasing concentrations and exposure time.

Keywords:

Poecilia, Poeciliidae, exposure, fibrosis, histopathology, necrosis, oils, skin, skin lesions, toxicity, toxicology, water pollution,

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1. Marty GD, Hoffmann A, Okihiro MS, Hepler K, Hanes D: Retrospective analysis: bile hydrocarbons and histopathology of demersal rockfish in Prince William Sound, Alaska, after the Exxon Valdez oil spill. Mar Environ Res, 56, 569-584, 2003.
2. Fent K: Ecotoxicological effects at contaminated sites. Toxicology, 205 (3): 223-240, 2004.
3. Carls MG, Rice SD, Hose JE: Sensitivity of fish embryos to weathered crude oil: Part I. Low-level exposure during incubation causes mal-formations, genetic damage, and mortality in larval Pacific herring (Clupea pallasi). Environ Toxicol Chem, 18 (3): 481-493, 1999.
4. Heintz RA, Short JW, Rice SD: Sensitivity of fish embryos to weathered crude oil: Part II. Increased mortality of pink salmon (Oncorhynchus gorbuscha) embryos incubating downstream from weathered Exxon Valdez crude oil. Environ Toxicol Chem, 18 (3): 494-503, 1999.
5. Heintz RA, Rice SD, Wertheimer AC, Bradshaw R, Thrower FP, Joyce JE, Short JW: Delayed effects on growth and marine survival of pink salmon Oncorhynchus gorbuscha after exposure to crude oil during embryonic development. Mar Ecol Prog Ser, 208, 205-216, 2000.
6. Peterson CH, Rice SD, Short JW, Esler D, Bodkin JL, Ballachey BE, Irons DB: Long-term ecosystem response to the Exxon Valdez oil spill. Science, 302 (5653): 2082-2086, 2003.
7. Incardona JP, Carls MG, Teraoka H, Brown DW, Collier TK, Scholz NL: Aryl hydrocarbon receptor-independent toxicity of weathered crude oil during fish development. Environ Health Perspect, 113 (12): 1755-1762, 2005.
8. Incardona JP, Collier TK, Scholz NL: Defects in cardiac function precede morphological abnormalities in fish embryos exposed to poly-cyclic aromatic hydrocarbons. Toxicol Appl Pharmacol, 196 (2): 191-205, 2004.
9. Berdougo E, Coleman H, Lee DH, Stainier DY, Yelon D: Mutation of weak atrium/atrial myosin heavy chain disrupts atrial function and influences ventricular morphogenesis in zebrafish. Development, 130 (24): 6121-6129, 2003.
10. Joseph EM: Zebrafish IRX1b in the embryonic cardiac ventricle. Dev Dyn, 231(4): 720-6, 2004.
11. Scott KK, Norris RA, Potter SS, Norrington DW, Baybo MA, Hicklin DM, Kern MJ: GeneChip microarrays facilitate identification of Protease Nexin-1 as a target gene of the Prx2 (S8) homeoprotein. Cell Biol, 22 (2): 95-105, 2003.
12. Cowart LA, Okamoto Y, Pinto FR, Gandy JL, Almeida JS, Hannun YA: Roles for sphingolipid biosynthesis in mediation of specific programs of the heat stress response determined through gene expression profiling. J Biol Chem, 278 (32): 30328-30338, 2003.
13. Brand DG, Fink R, Bengeyfield W, Birtwell IK, Mcallister CD: Salt water-acclimated pink salmon fry (Oncorhynchus gorbuscha) develop stress-related visceral lesions after 10-day exposure to sublethal concentrations of thewater-soluble fraction of north slope crude oil. Toxicol Pathol, 29 (5): 574-584, 2001.
14. Machado M R, Fanta E: Effects of Organophosphorus Metyl Parathion on the Branchial Epithelium of a Freshwater Fish Metynnis roosevelti. Braz Arch Biol Technol, 46(3): 361-372, 2003.
15. Akaishi FM, Silva de Assis HC, Jakobi SCG, Eiras-Stofella DR, St-Jean SD, Courtenay SC, Lima EF, Wagener ALR, Scofield AL, Oliveira Ribeiro CA: Morphological and neurotoxicological findings in Tropical Freshwater fish (Astyanax sp.) after waterborne and acute exposure to Water Soluble Fraction (WSF) of crude oil. Arch Environ Contam Toxicol, 46: 244–253, 2004.
16. Law M: Differential diagnosis of ulcerative lessions in fish. Environ Health Perspect, 109 (Suppl. l5): 681-686, 2001.
17. Önen Ö, Gündüz Ö, İşisağ Üçüncü S: Ham petrolün suda çözünebilen kısımlarının Pelvicachromis pulcher (Boulenger, 1901) (Cichlidae, Teleostei) bağırsak ve karaciğeri üzerindeki etkileri. Kafkas Univ Vet Fak Derg, 17 (Suppl-A): S105-S110, 2011.
18. Baumann PC: The use of tumors in wild fish to assess ecosystem health. Aquat Ecosyst Health, 1: 135-142, 1992.
19. Engel AG, Arahata K: Mononuclear cells in myopathies: quantitation of functionally distinct subsets, recognition of antigenspecific cell mediated cytotoxicity in some diseases, and implications for the pathogenesis of the different inflammatory myopathies. Hum Pathol, 17, 704-721, 1986.
20. Jerusalem F, Rakusa M, Engel AG, MacDonald RD: Morphometric analysis of skeletal muscle capillary ultrastructure in inflammatory myopathies. J Neurol Sci, 23, 391-401, 1974.
21. Whitaker JN, Engel WK: Vascular deposits of immunoglobulin and complement in idiopathic inflammatory myopathy. N Engl J Med, 286, 333-338, 1972.
22. Illa I, Nath A, Dalakas MC: Immunocytochemical and virological characteristics of HIV-associated inflammatory myopathies: similarities with seronegative polymyositis. Ann Neurol, 49, 474-481, 1991.
23. Emslie-Smith AM, Arahata K, Engel AG: Major histocompatibility complex class-I antigen expression, immunolocalization of interferon subtypes and T-cell-mediated cytotoxicity in myopathies. Hum Pathol, 20, 224-231, 1989.