AYŞEGÜL KUBİLAY, SONER ALTUN, Behire Işıl DİDİNEN, Seçil EKİCİ, Öznur DİLER

Gökkuşağı alabalığı (Oncorhyncus mykiss) işletmelerinde Flavobacterium psychrophilum izalosyonu

Dört faklı gökkuşağı alabalığı kuluçkahanesinden alınan örneklerde Flavobacterium psychrophilum’un ortaya çıkış noktaları ve bulaşma yolları incelenmiştir. Bu amaçla yumurta inkubasyon suyu, ovaryum sıvısı, sperm, döllenmemiş yumurta, döllenmiş yumurta, gözlenmiş yumurta ve larval dönemlerden örneklemeler yapılmıştır. Araştırma sonucunda, ovaryum sıvısında, inkübasyon suyunda ve yumurtada F. psycrophilum tespit edilmiş, sperm örneklerinde ise etkene rastlanmamıştır. Dolayısıyla anaç balıkların bu patojenin latent taşıyıcısı olabileceği kanısına varılmıştır. Araştırmada Fethiye bölgesinde 4 farklı gökkuşağı alabalığı kuluçkahanesinden Ocak-Şubat 2007 tarihlerinde F. psychrophilum’un toplamda 44 izolatı elde edilmiştir. Farklı işletmelerden izole edilen F. psychrophilum’un fenotipik karekterleri homojen bulunmuştur. Bu izolatların biyokimyasal ve fizyolojik özellikleri F.psychrophilum’u karakterize etmektedir. Enzim üretimleri API ZYM test kitleri kullanılarak yapılmıştır. API ZYM testleri sonucunda; F. psychrophilum izolatlarının proteolitik ve lipolitik enzimleri üretebilme yeteneğinde olduğu ancak karbonhidratları kullanamadığı tespit edilmiş, izolatların esteraz ve sistin arilamidaz enzim üretiminde de farklılık gösterdikleri saptanmıştır.

Anahtar Kelimeler:

izolasyon, kuluçkahane, balık çiftlikleri, balık yetiştiriciliği, balık yumurtası, fenotip, enzimler, esteraz, anaçlar, gökkuşağı alabalığı, yumurtalıklar, gizli enfeksiyonlar

Isolation of Flavobacterium psychrophilum in farmed rainbow trout (Oncorhynchus mykiss)

Occurrence of Flavobacterium psychrophilum at four rainbow trout hatcheries was investigated to proved more knowledge about the reservoirs and transmission of this bacterium. Incubating water, ovarian fluid, milt, unfertilized eggs, fertilized eggs, eyed eggs and fry stages were sampled. Flavobacterium psychrophilum was not found in milt while the pathogen was found ovarian fluid. Indicating that broodstock may serve as a reservoir and are latent carriers of the pathogen. F. psychrophilum was determined on eggs and from water samples. F. psychrophilum 44 isolates was isolated from four rainbow trout hatcheries in Fethiye region during January and February 2007. The F. psychrophilum isolates obtained from different farms proved to be homogeneously phenotipic characteristics. The F. psychrophilum isolates represented a biochemical and physiological characteristics were observed. Enzym production was tested using API ZYM strips. In the result of API ZYM test, the F. psychrophilum isolates produced lipolytic and proteolytic enzymes but not utilise simple or complex carbohydrates. Isolates from all of the four hatcheries studied showed differences in estrase and cyctin arylamidase production within a group.

Keywords:

isolation, hatcheries, fish farms, fish farming, fish eggs, phenotypes, enzymes, esterases, rootstocks, rainbow trout, ovaries, latent infections,

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