ONUR ATAKİŞİ, SENA ÇENESİZ, AYLA ÖZCAN, EMİNE ATAKİŞİ, Dilek Aksu ELMALI, Ahmet ÖNCÜER

Farkı miktarlarda meşe yaprağı ile beslenen kuzularda redükte glutatyon, malondialdehit, β-karoten ve retinol düzeylerinin araştırılması

Çalışmada, farklı miktarlarda tüketilen meşe yaprağının (Quercus hartwissiana), polietilen glikol (PEG) varlığında ya da yokluğunda, redükte glutatyon (GSH), malondialdehit (MDA), β-karoten ve retinol gibi oksidan/antioksidan sistemde rolü olan parametrelere etkisinin olup olmadığının araştırılması amaçlanmıştır. Çalışmada materyal olarak canlı ağırlık ortalaması 33.0±0.7 kg olan 42 kuzu kullanıldı. Her grupta 6 kuzu olacak şekilde 7 gruba ayrıldı. Deneme boyunca bireysel kafeslerinde barındırılan hayvanlara kuru madde bazında 272 g konsantre yem ve değişik miktarlarda kuru ot verildi. Grup 1: Standart rasyon (Kontrol), Grup 2: 185 g meşe yaprağı ve 0 g PEG; Grup 3: 185 g meşe yaprağı +10 g PEG; Grup 4: 185 g meşe yaprağı + 20 g PEG; Grup 5: 370 g meşe yaprağı; Grup 6: 370 g meşe yaprağı + 20 g PEG; Grup 7: 370 meşe yaprağı + 40 g PEG verilerek beslendi. Tüm hayvanlardan denemeye başlanıldığı gün (kontrol), 30. ve 60. günlerinde kan numuneleri toplandı. Tam kanda GSH, plazmada MDA, β-karoten ve retinol düzeyleri kolorimetrik olarak saptandı. Kaba yem kaynağı olarak katılan meşe yaprağının PEG varlığında ya da yokluğunda lipid peroksidasyonun belirteci olan MDA, antioksidan özelliğe sahip GSH, retinol ve β-karoten düzeylerinde istatistik olarak önemli bir farka neden olmadığı tespit edildi. Gruplar günlere göre mukayese edildiğinde sadece Grup 4’teki 60. gün MDA düzeyinin diğerlerine göre istatistik olarak düşük (P

Anahtar Kelimeler:

Quercus hartwissiana, beta-karoten, kan kimyası, diyetler, glutatyon, koyun, kuzu, malonaldehit, beslenme fizyolojisi, retinol

Reduced glutathione, malondialdehyde, β-carotene and retinol levels in lambs fed with various amounts of oak leaves

In this study, it is aimed to investigate the effects of different amounts of oak leaves (Quercus hartwissiana) consumed with or without polyethylene glycol (PEG) on the levels of reduced glutathione (GSH), malondialdehyde (MDA), β-carotene and retinol. Fourty two lambs with the average body weight 33.0±0.7 kg were used as the animal material in the study. The animals were divided into 7 groups each containing 6 lambs. The lambs housed in individual cages were fed with 272 g concentrate feed and various amounts of dried hay with the following feeding regimen: Group 1: Standard ration (Control); Group 2: 185 g oak leaf plus 0 g PEG; Group 3: 185 g oak leaf plus 10 g PEG; Group 4: 185 g oak leaf plus 20 g PEG; Group 5: 370 g oak leaf; Group 6: 370 g oak leaf plus 20 g PEG; Group 7: 370 g oak leaf plus 40 g PEG. Blood samples were collected from all animals on day 0 (control), 30 and 60. Levels of whole blood GSH, plasma MDA, β-carotene and retinol were measured colorimetrically. It was determined that oak leaves added to rations as roughage did not affect GSH, MDA, retinol and β-carotene levels in the presence or absence of PEG. In daily comparison of groups, only group 4 (60th day) had significantly decreased MDA levels as compared to others (P<0.05). According to the results, it could be concluded that oak leaves do not cause alterations in the selected antioxidant parameters in lambs.

Keywords:

Quercus hartwissiana, beta-carotene, blood chemistry, diets, glutathione, sheep, lambs, malonaldehyde, nutrition physiology, retinol,

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