ATİLA ATEŞ, FERAYE ESEN GÜRSEL, AYŞEN ALTINER, TANAY BİLAL, ÖZGE ERDOĞAN BAMAÇ, Hande ÖZYOĞURTÇU

Effect of Garcinia cambogia extract on fatty liver in rats fed high lipid

Garcinia cambogia hidroksisitrik asit içeren bir bitki olup, iştah ve vücut yağ birikimini azaltmaktadır. Bu çalışmayla yüksek lipid diyeti ile beslenen dişi ratlarda Garcinia cambogia ekstresinin karaciğer fosfolipid, trigliserit, lipid hidroperoksit, total protein düzeyleri ve gamma glutamil transferaz (GGT) aktivitesine olan etkilerini ve karaciğer histopatolojisini araştırılması amaçlanmıştır. Araştırma için 30 adet 1 yaşında Sprague-Dawley sıçan kullanılmış ve üç eşit gruba ayrılmışlardır. Grup 1 (kontrol grubu) %2 sıvı nebati yağ içeren standart yemle beslenmiştir. Grup 2 ve 3’ün yemlerinin her ikisi de yükse oranlarda nebati yağ (%2 sıvı ve %5 hidrojenize) ve kolesterol (%3) içermektedir. 45. günden itibaren 3. grubun yemine %4.5 (W/W) Garcinia cambogia ekstresi ilave edilmiştir. Sıçanlar 75. günün sonunda ötenazi edilmiştir. Karaciğer örnekleri, post mitokondriyal fraksiyon (PMF) elde etmek üzere tartılmış, homojenize edilmiş ve santrifüjlenmiştir. Kontrol grubuna kıyasla yüksek lipid diyetiyle beslenen grupta ve diğer iki gruba kıyasla Garcinia cambogia ilave edilmiş yemle beslenen grupta, PMF fosfolipid düzeyleri anlamlı olarak düşmüştür (P

Anahtar Kelimeler:

lipit, Garcinia cambogia, beslenme engelleyiciler, kan proteinleri, vücut yağı, kolesterol, diyetler, enzim aktivitesi, karaciğer yağlanması, yem zenginleştiriciler, gama-glutamiltransferaz, histopatoloji, lipid peroksit, fosfolipit, bitki özütü, bitkisel yağlar, sıçan, triyosilgliserol

Yüksek lipid diyetiyle beslenen sıçanlarda Garcinia cambogia ekstresinin yağlı karaciğer üzerine etkisi

Garcinia cambogia is a plant which contains hydroxycitric acid and suppresses appetite and body fat accumulation. The aim of the study was to investigate the improving effect of Garcinia cambogia-extract on liver phospholipid, triglyceride, lipid hydroperoxide, total protein and gamma-glutamyltransferase (GGT) activity, and histopathology of liver in female rats fed high-lipid diet. One-year-old 30 female Sprague-Dawley rats were separated to three equal groups. Group 1 (control group) was fed basal diet (2% liquid vegetable oil, 0% cholesterol), while the diets of both group 2 and 3 contained vegetable oil (2% liquid and 5% hydrogenated vegetable oil) and cholesterol (3%). 4.5% (W/W) Garcinia cambogia-extract was added to the diet of group 3 from day 45. Rats were euthanized on day 75. Liver samples were weighed, homogenized and centrifuged to obtain post-mitochondrial fractions (PMF). PMF phospholipid levels significantly decreased in the group fed high-lipid diet compared with the control group and in the group fed Garcinia cambogia-extract compared with the other two groups (P<0.05). PMF lipid hydroperoxide levels were significantly higher and PMF triglyceride levels were significantly lower in the control group than those in the other two groups (P<0.05). In the result of histopathological examinations, marked fat infiltration was observed in hepatocytes of animals fed high-lipid diet. Livers of animals fed Garcinia cambogia-added diet showed moderate fat infiltrations of the hepatocytes. Liver fattening partly occurred in rats fed high-lipid diet. However, this did not lead to the severe cellular degeneration. Garcinia cambogia added to the high-lipid diet insufficiently impaired liver fattening in the present dose.

Keywords:

lipids, Garcinia cambogia, antifeedants, blood proteins, body fat, cholesterol, diets, enzyme activity, fatty liver, feed supplements, gamma-glutamyltransferase, histopathology, lipid peroxides, phospholipids, plant extracts, plant oils, rats, triacylglycerols,

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