ALİ REHA AĞAOĞLU, MUSTAFA KAYMAZ, ÖZGECAN KORKMAZ AĞAOĞLU, KÜBRA KARAKAŞ ALKAN, İLKNUR PİR YAĞCI, UMUT TAŞDEMİR

Effect of different gonadotropin preparation on ovulatory response and embryo yield in Karayaka ewes

Sunulan çalışmada, Karayaka koyunlarında; oFSH (Ovagen®) ve pFSH (Folltropin®) uygulamalarının, süperovulasyon ve embriyo kalitesi üzerine etkilerinin karşılaştırılması amaçlandı. Bu çalışmada toplam 35 baş Karayaka koyunu kullanıldı. Koyunlar rastgele 2 gruba ayrıldı. Koyunların seksüel siklusları, 14 gün süreyle intravaginal CIDR kullanılarak senkronize edildi. Uygulamanın 12. gününden itibaren, 4 gün süreyle Grup I’e oFSH (GI, n=16), Grup II’ye pFSH (GII, n=19) uygulandı. CIDR çıkartıldıkan 6 gün sonra laparotomik olarak embriyo toplama işlemi ve ovaryum muayenesi yapıldı. Gruplar arasında, korpus luteum, fertilize olmamış oosit, embriyo, dondurulabilecek embriyo sayıları, ovulasyon, fertilizasyon, geri kazanım ve embriyo elde etme oranları arasında istatiksel olarak önemli bir fark bulunmadı. Diğer taraftan; gruplar arasında embriyo kaliteleri karşılaştırıldığında GI’den elde edilen embriyoların kalitesi daha yüksek bulundu (P

Anahtar Kelimeler:

FSH, Karayaka, sarı cisim, embriyo, koyunlar, gonadotropinler, oosit, ovulasyon, koyun, süperovulasyon

Karayaka koyunlarında farklı gonadotropin uygulamalarının embriyo verimleri ve ovulasyon yanıtları üzerine etkileri

In this study, the main goal was to investigate the effect of stimulation with either oFSH (Ovagen®) or pFSH (Folltropin®) on the superovulatory response and embryo quality in Karayaka ewes. Thirty-five ewes were used in this study. The ewes were randomly allocated to two groups. Estrous cycles in ewes were synchronized with the controlled integral drug release dispensers (CIDR®) for 14 days. Starting on day 12 and during 4 consecutive days ewes were treated with oFSH in Group I (GI, n=16) and pFSH in Group II (GII, n=19). Ovarian examination and embryo recoveries were carried out with the aid of laparotomy on day 6 following CIDR withdrawal. There was no significant differences in the number of corpora lutea (CL), unfertilized oocytes (UFOs), total number of embryos (EMBTot), total number of freezable embryos (FEMBTot), ovulation rate (OR), fertilization rate (FR), recovery rate (RR) and embryo recovery rate (ERR) between the groups. On the other hand, embryo quality was better in GI compared to GII (P<0.05). In conclusion, it has been thought that application of oFSH for superovulatory treatment in Karayaka ewes was more effective than pFSH for the quality of embryos.

Keywords:

FSH, Karayaka, corpus luteum, embryos, ewes, gonadotropins, oocytes, ovulation, sheep, superovulation,

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