Bülent BÜLBÜL, ŞÜKRÜ DURSUN, Mesut KIRBAŞ, MEHMET KÖSE, Seyit ÜMÜTLÜ

Düvelerde embriyo transferi öncesi flunixin meglumin uygulamasının gebelik oranı üzerine etkisi

Bu çalışma ile, östrüsün 7. gününde (östrüs 0) embriyo transferi öncesinde flunixin meglumin (non-steroid antiinflamatuar) enjeksiyonunun gebelik oranı üzerine etkisi araştırıldı. Bu amaçla 39 baş İsviçre Esmeri düve kullanıldı. Östrüs tespitini takiben 7. günde dondurulmuş-çözdürülmüş embriyolar taşıyıcı düvelere transfer edildi. Hayvanlar rastgele iki gruba ayrıldı ve çalışma grubuna (FM grubu, n = 20) transferden 5 dak önce IM yolla 500 mg flunixin meglumin enjeksiyonu yapılırken, diğer grup (Kontrol, n = 19) kontrol olarak bırakıldı ve 2 ml fizyolojik tuzlu su solüsyonu IM olarak enjekte edildi. Bütün hayvanlara dondurulmuş-çözdürülmüş blastosist aşamasında bulunan 1. kalite embriyolar transfer edildi. Transferler çözdürmeden sonraki 5 dak içinde, üst epidural anestezi yardımıyla, corpus luteumun bulunduğu taraftaki kornu uteriye gerçekleştirildi. Gebelik muayeneleri östrüsten sonraki 30. günde ultrason kullanılarak 7.5 MHz rektal prob ile yapıldı. FM ve kontrol gruplarında gebelik oranları %50.0 ve %52.6 olarak tespit edildi. Gruplar arasındaki fark istatistiki açıdan önemli bulunmadı. Düvelerde dondurulmuş-çözdürülmüş embriyo transferinden 5 dak önce IM yolla 500 mg flunixin meglumin uygulamasının gebelik oranlarına etkisinin olmadığı kanısına varıldı.

Anahtar Kelimeler:

hayvan yetiştiriciliği, embriyo nakli, dişi hayvanlar, fluniksin, düve, steroit olmayan antienflamatuar ajanlar, gebelik oranları, çözülme

The effect of flunixin meglumine injected before embryo transfer on pregnancy rates in heifers

In this study, the effect of flunixin meglumine (non-steroid anti-inflamatuar) injection before embryo transfer on d 7 (oestrus 0) on pregnancy rate was examined. For this aim, 39 Brown Swiss heifers were used. Following the oestrus detection, cryopreserved cattle embryos were transferred on d 7. Treatment animals were chosen randomly and injected intramuscularly 500 mg flunixin meglumine (FM group, n = 20) 5 min before embryo transfer application while the others remained as controls (Control, n = 19) and intramuscularly injected 2 ml of saline solution. All animals were transferred first quality cryopreserved blastosist embryos. Transfers were performed to uterine horn ipsilateral to the corpus luteum in 5 min after thawing with the help of epidural anesthesia. Pregnancy diagnoses were performed on d 30 by ultrasound with 7.5 MHz rectal probe. Pregnancy rates were 50.0% in FM group and 52.6% in Control group. The difference between groups was not significant. It is concluded that 500 mg flunixin meglumine injection 5 min before cryopreserved embryo transfer had no effect on pregnancy rates in heifers.

Keywords:

animal husbandry, embryo transfer, female animals, flunixin, heifers, non-steroidal antiinflammatory agents, pregnancy rate, thawing,

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