Detection of enterohemolysin and intimin genes in Escherichia coli O157:H7 strains isolated from calves and cattle in Afyonkarahisar - Turkey

Bu çalışmada, 457 dışkı örneğinden (237 buzağı ve 220 sığır) izole edilen 14 Escherichia coli O157:H7 suşunun enterohemolizin (EhlyA) ve intimin (eaeA) virulens genlerinin PZR ile belirlenmesi amaçlandı. On üç suşta (%92.8) EhlyA geni saptanırken, 8 suşta (%57.1) eaeA geni pozitifti. Sekiz eaeA geninin 4’ü (%50.0) ishalli, 2’si (%25.0) ishalsiz buzağılar ve 2’si de (%25.0) klinik olarak sağlıklı görünen sığırlarda belirlendi. Toplam 7 (%50.0) suşun hem EhlyA hem de eaeA geni taşıdığı belirlendi. Bu çalışma ile özellikle ishalli buzağıların ve sığırların, insanlar için patojenik olabilecek E. coli O157:H7 rezervuarı oldukları doğrulandı.

Afyonkarahisar’da sığır ve buzağılardan izole edilen Escherichia coli O157:H7 suşlarında intimin ve enterohemolizin Genlerinin belirlenmesi

The aim of this study was to detect enterohemolysin (EhlyA) and intimin (eaeA) virulence genes of 14 Escherichia coli O157:H7 strains isolated from 457 fecal samples (237 calves and 220 cattle) by PCR. While EhlyA gene was determined in 13 (92.8%) strains, the eaeA gene was positive in 8 (57.1%) strains. Of the 8 eaeA genes, 4 (50.0%) were obtained from diarrheic calves, 2 (25.0%) from non-diarrheic calves, and 2 (25.0%) from healthy cattle. A total of 7 (50.0%) strains were determined to harbour both of the EhlyA and eaeA genes. This study confirmed that calves, especially diarrheic, and cattle are a reservoir of E. coli O157:H7 strains that may be pathogenic for human.

___

  • 1. Gyles CL: Shiga toxin-producing Escherichia coli: An overview. J Anim Sci, 85, 45-62, 2007.
  • 2. Karmali MA, Gannon V, Sargeant JM: Verocytotoxin-producing Escherichia coli (VTEC). Vet Microbiol, 140, 360-370, 2010.
  • 3. Chapman PA, Siddons CA, Wright DJ, Norman P, Fox J, Crick E: Cattle as a possible source of verocytotoxin-producing Escherichia coli O157 infections in man. Epidemiol Infect, 111, 439-447, 1993.
  • 4. Osek J, Gallien P, Protz D: Characterization of Shiga toxin-producing Escherichia coli strains isolated from calves in Poland. Comp Immunol Microbiol Infect Dis, 23, 267-276, 2000.
  • 5. Rangel JM, Sparling PH, Crowe C, Griffin PM, Swerdlow DL: Epidemiology of Escherichia coli O157:H7 outbreaks, United States, 1982– 2002. Emerg Infect Dis, 11, 603-609, 2005.
  • 6. Belongia E, MacDonald K, Parham G, White K, Korlath J, Lobato D, Strand S, Casale S, Osterholm M: An outbreak of Escherichia coli O157:H7 colitis associated with consumption of precooked meat patties. J Infect Dis, 164, 338-343, 1991.
  • 7. Bielaszewska M, Janda J, Blahova K, Minarikova H, Jikova E, Karmali MA, Laubova J, Sikulova J, Preston MA, Khakhria R, Karch H, Klazorova H, Nye O: Human Escherichia coli O157:H7 infection associated with the consumption of unpasteurized goat’s milk. Epidemiol Infect, 119, 299-305, 1997.
  • 8. Nielsen EM, Scheutz F: Characterization of Escherichia coli 0157 isolates from Danish cattle and human patients by genotyping and presence and variants of virulence genes. Vet Microbiol, 88, 259-273, 2002.
  • 9. Kang SJ, Ryu SJ, Chae, JS, Eo SK, Woo GJ, Lee JH: Occurrence and characteristics of enterohemorrhagic Escherichia coli O157 in calves associated with diarrhoea. Vet Microbiol, 98, 323-328, 2004.
  • 10. Dean-Nystrom EA, Bosworth BT, Cray WC, Moon HW: Pathogenicity of Escherichia coli O157:H7 in the intestines of neonatal calves. Infect Immun, 65, 1842-1848, 1997.
  • 11. Beutin L, Geiger D, Steinruck H, Zimmermann S, Karch H: Virulence markers of Shiga-like toxin-producing Escherichia coli strains originating from healthy domestic animals of different species. J Clin Microbiol, 33, 631-635, 1995.
  • 12. Bauer ME, Welch RA: Characterization of an RTX Toxin from Entero- hemorrhagic Escherichia coli O157:H7. Infect Immun, 64, 167-175, 1996.
  • 13. Şeker E, Kuyucuoğlu Y, Sareyyüpoğlu B, Yardımcı H: PCR detection of Shiga toxins, enterohaemolysin and intimin virulence genes of Escherichia coli O157:H7 strains isolated from faeces of Anatolian water buffaloes in Turkey. Zoonoses and Public Hlth, 57, e33-e37, 2010.
  • 14. Wells JG, Davis BR, Wachsmuth IK, Riley LW, Remis RS, Sokolow R, Morris GK: Laboratory investigation of hemorrhagic colitis outbreaks associated with a rare Escherichia coli serotype. J Clin Microbiol, 18, 512- 520, 1983.
  • 15. Schmidt H, Beutin L, Karch H: Molecular analysis of the plasmid- encoded hemolysin of Escherichia coli O157:H7 strain EDL 933. Infect Immun, 63, 1055-1061, 1995.
  • 16. Batchelor M, Knutton S, Caprioli, Huter V, Zanial M, Dougan G, Frankel G: Development of a universal intimin antiserum and PCR primers. J Clin Microbiol, 37, 3822-3827, 1999.
  • 17. Dorn CR, Francis DH, Angrich EJ, Willgohs JA, Wilson RA, Collins JE, Jenke BH, Shawd SJ: Characteristics of vero cytotoxin producing Escherichia coli associated with intestinal colonization and diarrhea in calves. Vet Microbiol, 13, 149-159, 1993.
  • 18. Aslantaş Ö, Erdoğan S, Cantekin Z, Gülaçtı İ, Evrendilek GA: Isolation and characterization of verocytotoxin -producing Escherichia coli O157 from Turkish cattle. Int J Food Microbiol, 106, 338-342, 2006.
  • 19. Yilmaz A, Gun H, Ugur M, Turan N, Yimaz H: Detection and frequency of VT1, VT2 and eaeA genes in Escherichia coli O157 and O157:H7 strains isolated from cattle, cattle carcasses and abattoir environment in Istanbul. Int J Food Microbiol, 106, 213-217, 2006.
  • 20. Beutin L, Krause G, Zimmermann S, Kaulfuss S, Gleier K: Characterization of Shiga toxin-producing Escherichia coli strains isolated from human patients in Germany over a 3-year period. J Clin Microbiol, 42, 1099-1108, 2004.
  • 21. Mainil JG, Jacquemin ER, Kaeckenbeeck AE: Association between the effacing (eae) gene and Shiga-like toxin-encoding genes in Escherichia coli isolates from cattle. Am J Vet Res, 54, 1064-1068, 1993.
  • 22. Sandhu KS, Clark RC, McFadden K, Brouwer A, Louie M, Wilson J, Lior H, Gyles CL: Prevalence of the eaeA gene in verotoxigenic Escherichia coli strains from dairy cattle in Southwest Ontario. Epidemiol Infect, 116, 1-7, 1996.
  • 23. Wieler LH, Vieler E, Erpenstein C, Schlapp T, Steinrück H, Bauerfeind R, Byomi A, Baljer G: Shiga toxin-producing Escherichia coli strains from bovines: Association of adhesion with carriage of eae and other genes. J Clin Microbiol, 34, 2980-2984, 1996.
  • 24. Beutin L, Montenegro MA, Ørskov I, Ørskov F, Prada J, Zimmermann S, Stephan R: Close association of verotoxin (Shiga- like toxin) production with enterohemolysin production in strains of Escherichia coli. J Clin Microbiol, 27, 2559-2564, 1989.
  • 25. Gyles C, Johnson R, Gao A, Ziebell K, Pierard D, Aleksic S, Boerlin P: Association of enterohemorrhagic Escherichia coli hemolysin with serotypes of Shiga-like-toxin-producing Escherichia coli of human and bovine origins. Appl Environ Microbiol, 64, 4134-4141, 1998.
Kafkas Üniversitesi Veteriner Fakültesi Dergisi-Cover
  • ISSN: 1300-6045
  • Yayın Aralığı: Yılda 6 Sayı
  • Başlangıç: 1995
  • Yayıncı: Kafkas Üniv. Veteriner Fak.
Sayıdaki Diğer Makaleler

Effects of Nigella sativa aqueous extracts on gastric acid secretion in isolated rat stomach

AHMET TOPAL, FİKRET ÇELEBİ

Tavukçuluk Araştırma Enstitüsünde bulunan kahverengi yumurtacı saf hat horozlarda sperma kalitesinin değerlendirilmesi

ONGUN UYSAL, Zuhal DEMİR, ÇİĞDEM ÇEBİ ŞEN, Şahnur Erdoğan DEMİRTAŞ, MEHMET BORGA TIRPAN, Muhammet TUNCA, Serdar KAMANLI

Tapeworm infections in stray dogs and cats in İstanbul, Turkey

KEREM ÖTER, ZAHİDE BİLGİN, Recep TINAR, Erkut TÜZER

The effect of water temperature on standard and routine metabolic rate in two different sizes of Nile tilapia

HAKAN TÜRKER

Two new nematode species for Turkey Helmint fauna in roe deer (Capreolus capreolus), Spiculopteragia spiculoptera (Guschanskaia, 1931) and Minor Morph S. (Rinadia) mathevossiani (Ruchliadev, 1948)

ŞİNASİ UMUR, A. Tümay GÜRLER, Y. Emre BEYHAN, Cenk S. BÖLÜKBAŞ, MUSTAFA AÇICI

Japon bıldırcınlarında (Coturnix coturnix japonica) rasyona yüksek düzeylerde bakır ilavesinin tiroit hormonları ve bazı kan değerleri üzerine etkisi

Feyyaz ÖNDER, METİN ÇENESİZ, MEHMET KAYA, Metehan UZUN, Güler KARADEMİR

Practical diet includes low fish meal protein is able to sustain growth performance in gilthead sea bream (Sparus aurata, L.;1758) during the grow-out period

Özgür ALTAN, Aykut CİHANER

İki köpekte granüloza hücre tümörü

RECAİ TUNCA, Güneş SERİN, Erkmen Tuğrul EPİKMEN, AHMET AYDOĞAN, HAMDİ AVCI

Determination of lactic acid production and antagonistic activity against Helicobacter pylori of L. delbrueckii subsp. bulgaricus and S. thermophilus strains

BELMA ASLIM, Dilşad ONBAŞILI, Zehra Nur YÜKSEKDAĞ

Elazığ’da kırmızı et tüketim alışkanlıkları ve tüketicilerin hayvan refahı konusundaki görüşleri

İBRAHİM ŞEKER, ABDULLAH ÖZEN, HAKAN GÜLER, Pınar ŞEKER, İbrahim ÖZDEN