BİLAL AKYÜZ, Okan ERTUĞRUL, Evren KOBAN BAŞTANLAR

Blood protein polymorphism of horse types being bred by public at villages in three different regions of Turkey

Türkiye’de halk elinde yetiştirilen çeşitli at tipleri mevcuttur. Tarımda mekanizasyonun artması sonucunda Türkiye’de yetiştirilen yerli tip atların sayıları her geçen gün azalmaktadır. Çalışmada 35 baş Adapazarı, 30 baş Kars-Erzurum ve 20 baş Şanlıurfa menşeli toplam 85 baş yerli at kullanılmıştır. Çalışmada nişasta jel elektroforezi, poliakrilamid jel elektroforezi ve poliakrilamid izoelektrik odaklama yapılarak, albümin (ALB), transferin (TF), hemoglobin alfa (HBA), vitamin D-bağlayıcı protein (GC), serum karboksilesteraz (ES), A1B-glikoprotein (A1B), 6-fosfoglukonat dehidrojenaz (6-PGD) ve fosfoglukomutaz (PGM) olmak üzere sekiz sistem çalışılmıştır. İncelenen kan protein sistemlerinin gen frekansları direk hesaplama yöntemi ile hesaplanmıştır. Hesaplanan gen frekansları kullanılarak, bu sistemlere ait homozigotluk dereceleri ve ki-kare testi yapılmıştır. İncelenen üç farklı bölgedeki yerli atlarda çalışılan sekiz sistemin ki-kare test sonuçlarına göre 6-PGD sistemi, Kars-Erzurum ve Şanlıurfa bölgelerine ait yerli atlarda P

Anahtar Kelimeler:

fosfoglukonat dehidrogenaz, serum albumin, transferrin, D vitamini, coğrafi dağılım, glikoprotein, hemoglobin, genler, genetik polimorfizm, kan proteinleri, bağlayıcı proteinler, Türkiye, karboksiesteraz, at, lokuslar, yerli çiftlik hayvanları, fosfoglukomutaz

Türkiye'nin üç farklı bölgesinde halk elinde yetiştirilen yerli at tiplerinde kan protein polimorfizmi

Different breeds and types of horses are raised in different regions of Turkey. The numbers and types of native horses have declined steadily over time because of increased agricultural mechanization. The blood samples of 85 native Turkish horses of different types from different geographical regions (Kars-Erzurum 30, Şanlıurfa 20 and Adapazarı 35) were analysed for blood protein polymorphism. Starch gel electrophoresis, polyacrylamide gel electrophoresis and polyacrylamide isoelectric focusing were used to identify genotypic variants of albumin (ALB), transferrin (TF), hemoglobin alpha (HBA), vitamin D-binding protein (GC), serum carboxylesterase (ES), A1B-glycoprotein (A1B), 6-phosphogluconate dehyrogenase (6-PGD) and phosphoglucomutase (PGM) loci. The direct counting method was used to calculate the frequency of genes for blood protein systems. Homozygosity degrees and chi-square were calculated with the frequency of genes for blood protein systems. According to the results of chi-square testing on eight systems of three different regions, 6-PGD system was significant P<0.001 in the eastern population (Kars-Erzurum). Among the local horses in the south-east region (Şanlıurfa) 6-PGD system (P<0.001) and HBA system (P<0.05) were found to be significant. On the other hand, in the western region (Adapazarı), chi-square analyses of 6-PGD and HBA systems were found to be significant at P<0.05. In this study, it was found that the three different local horse types in the three regions descended from the same origin.

Keywords:

phosphogluconate dehydrogenase, serum albumin, transferrin, vitamin D, geographical distribution, glycoproteins, haemoglobin, genes, genetic polymorphism, blood proteins, binding proteins, Turkey, carboxylesterase, horses, loci, native livestock, phosphoglucomutase,

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