Farklı Etki Mekanizmalı İnsektisitlerin Tek Başına ve Karışım Halindeki Uygulamaların Galleria mellonella Larvalarında Hemosit Sayıları Üzerine Etkileri

Böcek büyüme düzenleyicileri ve mikrobiyal insektisitler zararlılarla mücadelede tarımsal alanlarda sıklıkla kullanılmaktadır. Bu nedenle, her iki insektisit türüne karşı zararlılarda direnç gelişimi meydana gelmektedir. Son yıllarda, direnç gelişimine karşı farklı etki mekanizmalı insektisitlerin karışım halinde uygulanması sıklıkla tercih edilen bir yöntem olarak kabul edilmektedir. İmmun sistem parametreleri, insektisitlerin zararlı böcek türleriyle mücadelede toksik etkilerinin belirlenmesinde biyobelirteç olarak kullanılmaktadır. Bu amaç kapsamında yapılan çalışmada juvenil hormon analoğu pyriproxyfen ve mikrobiyal insektisit B. thuringiensis’ in 50 ve 100 µg/mL derişimlerinin tek başına ve karışım halindeki (25+25 ve 50+50 µg/mL) uygulamalarının Galleria mellonella larvalarında total hemosit sayısı ve diferansiyel hemosit sayısı üzerine etkileri araştırılmıştır. Elde edilen bulgulara göre, pyriproxyfenin, G. mellonella larvalarında bağışıklık sistemini baskılayarak, B. thuringiensis biyopreparatının toksik etkisini arttırdığı belirlenmiştir.

Anahtar Kelimeler:

Bacillus thuringiensis, Galleria mellonella, İmmun sistem

The Effects of the Insecticides with Different Mode of Action Alone and in Mixture on Hemocyte Counts of Galleria mellonella Larvae

Insect growth regulators and microbial insecticides are frequently used in agricultural fields for pest management. Therefore, resistance development occurs in pests against both insecticide types. In recent years, the use of insecticides with different mode of action in mixture against resistance development has been accepted as a frequently preferred method. Immune system parameters are used as biomarkers to determine the toxic effects of insecticides in pest management. In this study, the effects of 50 and 100 µg/mL concentrations of the juvenile hormone analogue pyriproxyfen and the microbial insecticide Bacillus thuringiensis alone and in mixture (25+25 and 50+50 µg/mL) on total hemocyte count and differential hemocyte count in Galleria mellonella larvae were investigated. According to the findings, it was determined that pyriproxyfen increased the toxic effect of B. thuringiensis biopreparate by suppressing the immune system in G. mellonella larvae.

Keywords:

Bacillus thuringiensis Galleria mellonella, Immune system, pyriproxyfen,

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