Mustafa GÜNDOĞAN, Deniz YENİ, Fatih AVDATEK, Ömer HAZMAN

Koçlarda spermaya katılan CAPE'in (caffeic acid phenethyl ester) dondurma ve çözdürme sonrası spermatolojik parametreler, oksidatif stres ve DNA hasarı üzerine etkileri

Bu çalışmanın amacı koç sperma sulandırıcısına ilave edilen farklı dozlardaki caffeic acid phenethyl ester (CAPE) çözüm sonu spermatolojik parametreler, oksidatif stres ve DNA hasarı üzerine etkileri araştırıldı. Ejakulatlar beş baş Merinos koçtan haftada bir kez suni vajen yardımıyla toplandı ve bu işlem altı kere tekrarlandı. Ejakulatlar ml’de 150x106 spermatozoon olacak şekilde antioksidan içermeyen (kontrol) ve antioksidan içeren (10 µg/ml, 50 µg/ml ve 100 µg/ml) sulandırıcılar ile dört bölüme ayrıldı. Sulandırılan örnekler 0,25 ml’lik payetlerde 5 0C’de 3 saat ekilibrasyona tabi tutulduktan sonra sıvı azot buharında donduruldu. Subjektif motilite yönünden 50 ve 100 μg/ml içeren grupların, kontrol grubuna göre belirgin bir üstünlük sağladığı (P

Anahtar Kelimeler:

CAPE (caffeic acid phenethyl ester), dondurma, DNA hasarı, koç, oksidatif stres

The effects of CAPE (caffeic acid phenethyl ester) addition on spermatological parameters, oxidative stress and DNA damage after frozen-thawed process in ram semen.

The aim of this study is to investigate the effects of different doses caffeic acid phenethyl ester (CAPE) adding to extender on some spermatological parameters, oxidative stress and DNA damage during post-thawed of ram semen. Ejaculates were collected from five Merino rams using an artificial vagina ones a week and this process was repeated six times. Ejaculates were split into four aliquots and diluted to a final concentration of 150x106 spermatozoa/ml with the base extender containing antioxidant (10 µg/ml, 50 µg/ml and 100 µg/ml)and no additive (control). All samples were cooled to 4°C and equilibrated for 3 h then were loaded into 0.25 ml straws and frozen using a liqued nitrogen vapour and plunged into liquid nitrogen. In terms of subjective motility, the groups containing 50 and 100 μg / ml were found to be significantly superior (P

Keywords:

CAPE (caffeic acid phenethyl ester), frozen, DNA damage, ram, oxidative stres,

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