Eyüp BAĞCI, Fethi Ahmet ÖZDEMİR, Ömer KILIÇ

In Vitro Bulb Regeneration from Stem Explants of Endemic Geophyt Muscari aucheri (Boiss.) Baker

Muscari aucheri (Boiss.) Baker bears beautiful and attractive purplish blue flowers bloom between May and June; it grows stony slopes, mountain pastures an altitude of 1000-3000 meters. The natural populations of Muscari aucheri, is seriously affected by increased environmental pollution, urbanization and grazing. Therefore, this study aimed to accelerate multiplication by devising a strategy for an efficient in vitro bulblet regeneration system of Muscari aucheri using stem explants on MS medium containing 1, 3, 5 mg/l TDZ plus 0, 0.1, 0.2, 0.4 mg/l NAA (12 combinations). The stem explants induced direct bulblet regeneration on explants. Maximum mean number of bulblets per explant was noted on MS medium containing 3 mg/l TDZ + 0.4 mg/l NAA and maximum mean number of bulb diameter was noted on MS medium containing 5 mg/l TDZ + 0.2 mg/l NAA. The regenerated bulblets were isolated from stem explants and cultured on MS medium containing 40 g/l sucrose; where they gained diameter and rooted

Anahtar Kelimeler:

Muscari aucheri, in vitro, bulb regeneration

In Vitro Bulb Regeneration from Stem Explants of Endemic Geophyt Muscari aucheri (Boiss.) Baker

Keywords:

Muscari aucheri, in vitro, bulb regeneration,

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Ekim, T. (2006). Türkiye’nin bitkileri. Türkiye’nin Önemli Doga Alanları. Doga Dernegi, Ankara, Turkey, 47-48.
Jafari, A., Maassoumi, A.A., & Farsi, M. (2008). Karyological study on Bellevalia and Muscari (Liliaceae) species of Iran. Asian Journal of Plant Sciences, 7, 50-59.
Demirci, S., Özhatay, N., Koçyiğit, M. (2013). Muscari erdalii, a new species from Southern Turkey. Phytotaxa, 154, 38-46.
Ekim, T., Koyuncu, M., Vural, M., Duman, H., Aytac, Z., & Adıguzel, N. (2000). Türkiye Bitkileri Kırmızı Kitabı. Turkiye Tabiatını Koruma Dernegi Yayınları, Ankara.
Anonim, www.tubives.com
Davis, P.H., (1984). Flora of Turkey and East Aegean Islands, University Press, Edinburgh, 8.
Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15, 473-497.
Ochatt, S.J., Conreux, C., & Jacas, L. (2013). Flow cytometry distinction between species and between landraces within Lathyrus species and assessment of true-to-typeness of in vitro regenerants. Plant Systematic Evoluation, 299, 75-85.
Malabadi, R.B., & Van Staden, J. (2004). Regeneration of Ornithogalum in vitro. South African Journal of Botany, 70, 618-621.
Suh, J., Lee, W., Lee, A. (2005). New plantlet proliferation and bulbing promotion in vitro cultures of Ornithogalum hybrid. Acta Horticulturae, 683, 155-163.
Uranbey, S. (2010). Stimulating effects of different basal media and cytokinin types on regeneration of M. aucheri. Archives Biological Sciences, 62, 663-667.
Huetteman, C.A., Preece, J.E. (1993). Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell, Tissue and Organ Culture, 33, 105-119.
Nasircilar, A., Mirici, S., Karagüzel, Ö., Eren, Ö., Baktir, I. (2011). In vitro propagation of Muscari mirum from different explant types. Turkish Journal of Botany, 35, 37-43.
Suzuki, S., Nanako, M. (2001). Organogenesis and somatic embryogenesis from callus cultures in Muscari armeniacum. Leichtl. ex Bak. In vitro Cell, 37, 382-387.