Nezahat AKPOLAT, İbrahim Halil YILDIRIM, Mutalip ÇİÇEK, Şebnem NERGİZ, Özgür EZİN, Nida ÖZCAN, Mehmet HARMAN
Identification of causative species in patients with cuteneous leishmaniasis in Diyarbakır by polymerase chain reaction (pcr)-restriction fragment lenght polymorphism (RFLP)
Identification of causative species in patients with cuteneous leishmaniasis in Diyarbakır by polymerase chain reaction (pcr)-restriction fragment lenght polymorphism (RFLP)
Background: Leishmaniasis is a protozoan disease caused by more than twenty Leishmania species which are transmitted by infected phlebotomine sandflies to humans. Leishmaniasis include multiple clinical syndromes such as visceral , mucosal and cutaneous forms. Cutaneous Leishmaniasis (CL) is the most common and endemic form in southeastern Turkey. Purpose: CL can be caused by L. major, L. tropica, L. mexicana and L. amazonensis. Microscopy remains to be the standard diagnostic method because of its cheapness, ease of application and high specificity . Microscopic examination of smears, parasite culture and serological tests are performed for diagnosis. Recently several molecular methods, especially those based on the Polymerase chain reaction (PCR) have been developed for identification of Leishmania species. Method: In this study, 150 smear samples taken from patients with clinical findings of CL were studied by PCR- RFLP (restriction fragment length polymorphism) Results: Leishmania tropica was detected in 128 smear sample. L. tropica was found to be the most common species causing CL in southeastern Turkey. Conclusion: Identification of Leishmania species is important because different types may require different treatments. Determining the common species in the region may lead to develop treatment protocols.
Keywords:
Cutaneous Leishmaniasis, Polymerase Chain Reaction (PCR), Restriction Fragment Lenght Polymorphısm (RFLP),
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- Reithinger R, Dujardin JC., Molecular Diagnosis of Leishmaniasis: Current Status and Future Applications. Journal of Clinical Microbiology 2007: 21-25 Health 2. World Organization web sayfası
- (http://www.who.int/tdr/diseases/Leish,Progress 2003
- ,17thProgrammeReport) Erişim Tarihi: 05.12.2007
- Özbel Y, Töz ÖS, Leishmaniosis, Özcel MA. Tıbbi Parazit Hastalıkları 1. Baskı, İzmir: Mete Basım Matbaacılık Hizmetleri, 2007: 198-230
- Gangneux JP, Menotti J, Lorenzo F, Sarfati C, Blanche H, Bui H, Pratlong F, Garin YJF, Derouin F. Prospective Value of PCR Amplification and Seqencing for Diagnosis and Typing of Old World Leishmania Infections in an Area of Nonendemicity.Journal of Clinacal Microbiology.2003:1419-1422.
- Gürel MS, Ulukanlıgil M, Özbilge H, 2002. Cutaneous Leishmaniasis in Sanliurfa: epidemiologic and clinical features of the last four years (1997-2000). Int J Dermatol, 41: 1-6.
- Ertem M, Aytekin S, Acemoğlu H, Akpolat N. Aytekin N. Diyarbakır Dicle İlçesi Dedeköy ve Durabeyli’de Kutanöz Leishmaniasis Olgularının İncelenmesi.Türkiye Parazitoloji Dergisi. 2004 28 (2): 65-68
- Uzun S, Yücel A, Özpoyraz M.Cutaneous Leishmaniasis: Evalution of 3074 cases in the Çukurova Region,Turkey. Tibbi Parasitoloji Dergisi. 1997:21:142.
- ÖzpoyrazM, Memişoğlu HR.Kutanöz Leishmaniasis Türkiyedeki durumu.Arşiv 1996:5:1-5.
- Bensoussan, E., A. Nasereddin, F. Jonas, L. F. Schnur, and C. L. Jaffe. 2006. Comparison of PCR assays for diagnosis of cutaneous leishmaniasis. J. Clin. Microbiol. 44:1435-1439. [PMC free article][PubMed]
- Weigle KA, Valderlama L, Arias AL, Santrica C, Saravia NG. Leishmnanin standardization and evalution af safety,dose,storage,longevity of reaction and sensitization. Am Journal Tropica Medical Hyeneg. 1991:44:260-271.
- Piarroux R, Gambarelli F, Duman H, Fontes M, Dunan S, Mary C, Toga B, Qulici M. Comparison of PCR with Direct Examination of Bone Marrow Aspiration,Myeloulture and Serology for Diagnosis of Visceral Leishmniasis in Immunocompromised Patients. Journal of Clincal Microbiology.1994:746-749.
- Piarroux R, Gambarelli F, Duman H, Fontes M, Dunan S, Mary C, Toga B, Qulici M. Comparison of PCR with Direct Examination of Bone Marrow Aspiration,Myeloulture and Serology for Diagnosis of Visceral Leishmniasis in Immunocompromised Patients. Journal of Clincal Microbiology.1994:746-749.
- Demirel R, Erdoğan S. Determination of high risk regions of cutaneous leishmaniasis in Turkey using spatial analysis. Turkiye Parazitol Derg. 2009;33(1):8-14.
- Singh,S., A.Dey, and R. Sivakumar. 2005. Applications of molecular methods for Leishmaniacontrol. Exp. Rev. Mol. Diagn. 5:251-265. [PubMed].
- Zeyrek F, Korkmaz M, Ozbel Y. Serodiagnosis of anthroponotic cutaneous leishmaniasis (ACL) caused by Leishmania tropica in Sanliurfa Province, Turkey, where ACL Is highly endemic. Clin Vaccine Immunol 2007 Nov 29;14(11):1409-15. Epub 2007 Aug 29
- Dilmeç F, Matur A, Uzun S, Karakaş M, Memişoğlu HR. Çukurova ve Şanlıurfa Bölgelerinde Deri Lezyonlarından İzole Edilen Leishmania sp. DNA’larının Restriksiyon Endonükleazlarla Karşılaştırılması. Harran Tıp Fakültesi Dergisi. 2004; 1: 21-27.
- Kutanöz Leyişmanyozlu Hastalarda Etken Türlerin Pcr-Rflp Yöntemi İle Tanımlanması ,Fadime Eroğlu,yüksek lisans tezi. Danışman: Prof. Dr. İ. Soner Koltaş,ADANA-2008
- Yıldız Z. F, Gurses G, Uluca1 N, Yentur D N, Toprak Ş, Yeşilova Y, Culha5 G. Turkiye Parazitol Derg .2014; 38: 270-4
- ISSN: 2146-6033
- Yayın Aralığı: Yılda 2 Sayı
- Başlangıç: 2011
- Yayıncı: Veysi AKPOLAT