In-Vitro Nitric Oxide Scavenging and Anti-Tyrosinase Activity of Two Convolvulus L. Species

Convolvulus L. is a member of Convolvulaceae family which have been utilized for their biological activities in folk medicine. The aim of this study is to investigate the inhibitory effect on tyrosinase activity of two Convolvulus species as well as their nitric oxide (NO) scavenging activity potentials. Up to now, no reports have been found on their anti-tyrosinase and NO scavenging activity properties in the literature. C. galaticus and C. phrygius aerial parts were extracted with methanol and then NO scavenging activity assay was utilized for the assurance of radical scavenging activity of the extracts. Anti-tyrosinase activity of the extracts was determined using the modified 96-well microplate method. The highest NO scavenging and anti-tyrosinase activity were found in C. galaticus as 55.20% and 53.16%, respectively.

Anahtar Kelimeler:

Convolvulus L., nitric oxide scavenging activity, plant extract, tyrosinase inhibition

İki Convolvulus L. Türünün In-Vitro Nitrik Oksit Süpürme ve Anti-Tirozinaz Aktivitesi

Convolvulus L., Convolvulaceae familyasının bir üyesi olup, biyolojik aktivitelerinden dolayı halk arasında tıbbi amaçlı kullanılmaktadır. Bu çalışmanın amacı, iki Convolvulus türünün tirozinaz aktivite üzerindeki inhibitör etkisi ve nitrik oksit (NO) süpürme aktivite potansiyellerini belirlemektir. Şimdiye kadar yapılan yayınlarda bu iki bitkinin anti-tirozinaz ve NO süpürme aktivite özelliklerine ilişkin bir rapor bulunamamıştır. C. galaticus ve C. phrygius toprak üstü kısımları metanolde ekstrakte edildikten sonra ekstraktların radikal süpürme aktivitelerinin belirlenmesi için NO süpürme aktivite deneyi kullanıldı. Ekstraktların anti-tirozinaz aktivitesi, 96-kuyucuklu mikroplaka metodunun modifiye edilmesiyle belirlendi. En yüksek NO süpürme ve anti-tirozinaz aktivitesi C. galaticus'ta sırasıyla % 55.20 ve % 53.16 olarak bulundu.

Keywords:

Convolvulus L., nitrik oksit süpürme aktivitesi, bitki ekstraktı, tirozinaz inhibisyonu,

PDF

___

Amaeze, O.U., Ayoola, G.A., Sofidiya, M.O., Adepoju- Bello, A.A., Adegoke, A.O., Coker, H.A.B., 2011, Evaluation of antioxidant activity of Tetracarpidium conophorum (Mull. Arg) Hutch & Dalziel leaves, Oxidative Medicine and Cellular Longevity, Article ID 976701, 7 pages.
Atta, A.H., El-Sooud, K.A., 2004, Antinociceptive effect of some Egyptian medicinal plant extracts, Journal of Ethnopharmacology,95, 235-238.
Atta, A.H., Mohamed, N.H., Nasr, S.M., Mouneir, S.M., 2007, Phytochemical and pharmacological studies on Convolvulus fatmensis Ktze., Journal of Natural Remedies, 7, 109-119.
Aykurt, C., Sümbül, H., 2010, Varieties and chorology of C. oleifolius Desr. (Convolvulaceae) in Turkey. Biological Diversity and Conservation, 3(2), 155-162.
Balakrishnan, N., Panda, A.B., Raj, N.R., Shrivastava, A., Prathani, R.B., 2009, The evaluation of nitric oxide scavenging activity of Acalypha indicaLinn Root, Asian Journal Research Chemistry, 2(2), 148-150.
Baruah, N.C., Das, S., Yadav, S.K., 2014, Preliminary Phyto-chemical Analysis and Antioxidant Activities of Methanol Extract of Argyreia roxburghii Choisy, International Journal of Herbal Medicine, 2(1), 126-131.
Bhaskar, H.V. and Balakrishnan, N., 2009, In vitro antioxidant property of laticiferous plant species from Western Ghats Tamilnadu, India, International Journal of Health Research, 2(2), 163-170.
Bihaqi, S.W., Sharma, M., Singh, A.P., Tiwari, M., 2009, Neuroprotective role of Convolvulus pluricaulis on aluminium induced neurotoxicity in rat brain, Journal of Ethnopharmacology,124,409-415.
Cronquist, A., 1981, An Integrated System of Classification of Flowering Plants. Columbia University Press, NewYork.
Dhingra, D. and Valecha, R., 2007, Evaluation of the antidepressant-like activity of Convolvulus pluricaulis choisy in the mouse forced swim and tail suspention tests. Medical Science Monitor, 13, 155-161.
El-Shazlya, A. and Wink, M., 2008, Tropane and pyrrolidine alkaloids from Convolvulus lanatus Vahl, Zeitschrift für Naturforschung, 63c, 321-325.
Friedman, M., 1996, Food browning and its prevention: An overview. Journal of Agricultural and Food Chemistry, 44, 631-653.
Gomathi, D., Ravikumar, G., Kalaiselvi, M., Vidya, B., Uma, C., 2015, In vitro free radical scavenging activity of ethanolic extract of the whole plant of Evolvulus alsinoides (L.) L. Chinese Journal of Integrative Medicine, 21(6), 453-458.
Karioti, A., Protopappa, A., Megoulas, N., Skaltsa, H., 2007, Identification of tyrosinase inhibitors from Marrubium velutinum and Marrubium cylleneum, Bioorganic & Medicinal Chemistry, 15, 2708-2714.
Lakhanpal, P. and Rai, D.K., 2007, Quercetin: a versatile flavonoid, Internet Journal of Medical Update, 2(2), 22-37.
Lim, T.Y., Lim, Y.Y., Yule, C.M., 2009, Evaluation of antioxidant, antibacterial and antityrosinase activities of four Macaranga species, Food Chemistry, 114, 594-599.
Marcocci, L., Maguire, J.J., Droy-Lefaix, M.T., Packer, L., 1994, The nitric oxide scavenging properties of Ginkgo bilobaextract EGb 76J, Biochemical and Biophysical Research Communications, 201, 748-755.
Masuda, T., Yamashita, D., Takeda, Y., Yonemori, S., 2005, Screening for tyrosinase inhibitors among extracts of seashore plants and identification of potent inhibitors from Garciniasubelliptica, Bioscience Biotechnology and Biochemistry, 69(1), 197-201.
Nacef, S., Jannet, H.B., Abreu, P. and Mighri, Z., 2010, Phenolic constituents of Convolvulus dorycnium L. flowers, Phytochemistry Letters, 3, 66-69.
Nagmoti, D.M., Khatri, D.K., Juvekar, P.R., Juvekar, A.R., 2011, Antioxidant activity and free radical-scavenging potential of Pithecellobium dulce Benth seed extracts, Free Radical andAntioxidants, 2(2), 37-43. No, J.K., Soung, D.Y., Kim, Y.J., Shim, K.H., Jun, Y.S., Rhee, S.H., 1999, Inhibition of tyrosinase by green tea components, Life Science, 65, 241-246.
Ozay, C., Mammadov, R., Tasdelen, G., Karagur, E.R., Akca, H., 2015, Potential antioxidant, antiproliferative and hepatoprotective effects of Crataegus meyeri, Journal of Food Biochemistry,39,548-553.
Sadeghi-Aliabadi, H., Ghasemi, N., Kohi, M., 2008, Cytotoxic effect of Convolvulus arvensis extracts on human cancerous cell line. Research in Pharmaceutical Sciences,3(1), 31-34.
Taiz, L. and Zeiger, E., 1998, Plant Physiology, 2nd Edition, Sinauer Associates Publishers, pp. 778, Massachusetts.
Tawaha, K., Alali, F.Q., Gharaibeh, M., Mohammad, M., El-Elimat, T., 2007, Antioxidant activity and total phenolic content of selected Jordanian plant species, Food Chemistry, 104, 1372-1378.
Todd, F.G., Stermitz, F.R., Schultheis, P., Knight, A.P., Traub-Dargatz, J., 1995, Tropane alkaloids and toxicity of Convolvulus arvensis, Phytochemistry,39, 301-303.