Structure of midgut and peritrophic matrix in the last instar larvae of culex pipiens (Diptera: Culicidae)

Culex pipens'ın larval ortabağırsağı ve peritrofik matriks (PM)'i ışık ve elektron mikroskobuyla incelenmiştir. Önbağırsak ve anterior ortabağırsak birlikte, kardiya olarak isimlendirilen özel bir katlanma bölgesi oluştururlar. Kardiya ve gastrik çekumlar toraksta bulunur. Gastrik çekumlardan sonra, birinci ve beşinci abdomen segmentleri arasında uzanan abdominal ortabağırsak gelir. Yukarıda sözü edilen yapıların lümene bakan tarafları tek tabakalı epitelle döşelidir. Ancak, hücresel büyüklükleri ve boyanma özellikleri farklılık göstermektedir. Kardiyada yer alan anterior ortabağırsak hücreleri PM'i salgılar. PM, besin kitlesini çepeçevre sarar ve beşinci abdomen segmentinin sonuna kadar uzanır. Ortabağırsak epitel hücrelerindeki Golgi kanalları hücrenin apikal zarına diktir ve periferal sitoplazmada hücresel organeller azdır. PM'nin elektron mikrograflannda elektronca yoğun granüler ve elektronca az yoğun mikrofibrilli tabakalar görülür. Kalın olan mikrofibrilli tabaka ise elektronca yoğun iki tabaka tarafından üç bölgeye ayrılır.

Culex pipiens (Diptera: Culicidae)' in son evre larvalarında ortabağırsak ve peritrofik matriksin yapısı

Larval midgut and peritrophic matrix (PM) of Culex pipiens were investigated by light and electron microscope. Foregut and anterior midgut together form the special folding area called cardia. Cardia and gastric caeca are located in the thorax. Abdominal midgut follows the gastric caeca and lies between the first and the fifth abdominal segments. The luminal surfaces of the structures mentioned above are lined by single layered epithelial cells. However, the cell sizes and staining properties exhibit differences. The epithelial cells of the anterior midgut which are located in the cardia, produce PM. It surrounds the food bolus and lies to the end of the fifth abdominal segment. Golgi canals of midgut epithelial cells are upright to the apical membrane of the cell and the cellular organelles are seldom in peripheral cytoplasm. Electron-dense granular and electron-lucent microfibrillated layers are seen in the electron micrograph of PM. The thick microfibrillated layer is separated into three regions by electron dense bilayer

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