Esra CEBECİ, H. Filiz BOYACI, Yıldız DOĞAN, Laura TOPPINO, Giuseppe L. ROTINO

Determination of Heat and Drought Tolerant Lines in Segregating Populations Produced by Interspecific Crosses in Eggplant

Nowadays abiotic stresses originated by climate change are one of the main factors causing reductions to the agricultural production. Heat and drought are the most prominent abiotic stress factors affecting both eggplant production worldwide and food security. Although eggplant is known as more tolerant to these stresses compared to other vegetables and solanaceous crops, its quality and yield suffers from severe stress conditions. In this research, 256 F2 plants developed from the interspecific cross between the wild relative of Solanum insanum L. and the pure line (BATEM-TDC47) from Batı Akdeniz Agricultural Research Institute, (BATEM) eggplant gene pool were used as plant materials. Seedlings at 3-4 true leaves stage bred in three-liter pots filled with a 1:1 mixture of peat and perlite were subjected to drought stress test to this end, a 75% deficit irrigation was applied to the plants, while control plants were irrigated with the required amount to recover the 100% of ETp as appropriate management strategy. The stress symptoms of plants were determined by morphological and chemical analyses. Plant heights were measured on the 25th day of the experiment and visual evaluation stress symptoms was observed according to the 0-5 scale. Morphological observations, MDA (malondialdehyde) and proline content of selected plants were performed to confirm their tolerance levels to heat and drought. Following the drought test, 100 F2 lines, which were selected as drought tolerant, were transferred to the greenhouse for determination of heat tolerant individuals.

Keywords:

biotic stress drought, eggplant, heat, MDA, proline,

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Ajayi AT, Gbadamosi AE and Olumekun VO, (2018). Screening for drought tolerance in cowpea (Vigna unguiculata L, Walp) at seedling stage under screen house condition. International Journal of BioSciences & Technology, 11(1), 1-10.
Banik P, Zeng W, Tai H, Bizimungu B and Tanino K, (2016). Effects of drought acclimation on drought stress resistance in potato (Solanum tuberosum L.) genotypes. Environmental and Experimental Botany, 126:76-89 http://dx,doi,org/10,1016/ j,envexpbot, 2016,01,008
Barchi L, Portis E, Lanteri S, Alonso D, Diez MJ, Prohens J and Giuliano G, (2022). Worldwide population structure of eggplant identified by SPET genotyping over 3,400 accessions. Virtual Plant and Animal Genome XXIX Conference, Jan 2022, San Diego (CA), United States. Hal- 03551609.
Bates LS, Waldren RP and Teare ID, (1973). Rapid determination of free proline for water-stress studies. Plant and Soil, 39(1):205-207. Behboudian MH, (1977). Responses of eggplant to drought. I. Plant water balance. Scientia Horticulturae, 7(4):303-310.
Boyaci HF, Topcu V, Tepe A, Yildirim IK, Oten M and Aktas A, (2015). Morphological and molecular characterization and relationships of Turkish local eggplant heirlooms. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 43(1):100-107.
Boyaci HF, (2020). Development of new valuable introgression lines from the interspecific cross in eggplant (Solanum melongena L.). Applied Ecology and Environmental Research, 18(1):1771-1781.
Diaz-Perez JC and Eaton TE, (2015). Eggplant (Solanum melongena L.) plant growth and fruit yield as affected by drip irrigation rate. HortScience, 50(11):1709-1714.
Espanani S, Majidi MM, Saeidi G and Alaei H, (2019). Physiological aspects of inter-specific gene introgression to improve drought tolerance in safflower. Euphytica, 215(10):1-18.
Fahad S, Bajwa AA, Nazir U, Anjum SA, Farooq A, Zohaib A, Sadia S Nasim W, Adkins S, Saud S, Ihsan MZ, Alharby H, Wu C, Wang D and Huang J, (2017). Crop production under drought and heat stress: plant responses and management options, Frontiers in plant science, 8:1147.
Faiz H, Ayyub CM, Khan RW and Ahmad R, (2020). Morphological, physiological and biochemical responses of eggplant (Solanum melongena L.) seedling to heat stress. Pakistan Journal of Agricultural Sciences, 57(2):371-380
Fita A, Fioruci F, Plazas M, Rodriguez-Burruezo A and Prohens J, (2015). Drought tolerance among accessions of eggplant and related species. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Horticulture.72:461-462
Fowler C, Moore G and Hawtin GC, (2003). The international treaty on plant genetic resources for food and agriculture: A primer for the future harvest centres of the CGIAR, International Plant Genetic Resources Institute, Rome, Italy.
Frary A, Frary A, Daunay MC, Huvenaars K, Mank R and Doğanlar S, (2014). QTL hotspots in eggplant (Solanum melongena) detected with a high resolution map and CIM analysis. Euphytica, 197(2):211-228.
Gaufichon L, Prioul J and Bachelier B, (2010). What are the prospects for genetic improvement in drought-tolerant crops? Foundation Farm press 52 p.
Hoagland D and Arnon DI, (1950). The water culture method for growing plants without soil, California Agricultural Experiment Station, 347(2nd edit) 39 p., Newyork.
Karam F, Saliba R, Skaf S, Breidy J, Rouphael Y, and Balendonck J, (2011). Yield and water use of eggplants (Solanum melongena L,) under full and deficit irrigation regimes, Agricultural Water Management, 98(8):1307-1316.
Kandemir D, Balkaya A, Taşan M, Kobal Bekar N, Cemek B and Teksöz E, (2018). Nitelikli taze fasulye hatlarının kuraklığa dayanım düzeylerinin belirlenmesi ve kuraklık stresinde geliştirdikleri savunma mekanizmalarının ı̇ ncelenmesi, TÜBITAK TOVAG 116O881 No'lu Proje Raporu, 142s. (in Turkish)
Kıran S, Kuşvuran Ş, Özkay F and Ellialtıoğlu ŞŞ, (2015). Domates, patlıcan ve kavun genotiplerinin kuraklığa dayanım durumlarını belirlemeye yönelik olarak incelenen özellikler arasındaki ilişkiler. Nevşehir Bilim ve Teknoloji Dergisi, 4(2):9-25. (in Turkish)
Kıran S, Kuşvuran Ş. Özkay F and Ellialtioglu ŞŞ, (2019). Change of physiological and biochemical parameters under drought stress in salt-tolerant and salt-susceptible eggplant genotypes. Turkish Journal of Agriculture and Forestry 43(6):593- 602
Kouassi B, Prohens J, Gramazio P, Kouassi AB, Vilanova S, Galán-Ávila A and Plazas M, (2016). Development of backcross generations and new interspecific hybrid combinations for introgression breeding in eggplant (Solanum melongena), Scientia Horticulturae 213:199- 207, doi:10,1016/j,scienta,2016,10,039.
Knapp S, Vorontosova M and Prohens J, (2013). Wild relatives of eggplant (Solanum melongena L.:Solanaceae): New understanding of species names in a complex group, PLOS ONE 8:e57039.
Kuşvuran Ş, (2010). Kavunlarda kuraklık ve tuzluluğa toleransın fizyolojik mekanizmaları arasındaki bağlantılar, Çukurova Üniversitesi Fen Bilimleri Enstitüsü Bahçe Bitkileri Anabilim Dalı, Adana. 377 s. (in Turkish)
Li Y, Li Z, Luo S and Sun B, (2011). Effects of heat stress on gene expression in eggplant (Solanum melongena L.) seedlings, African Journal of Biotechnology, 10(79):18078-18084.
Lum MS, Hanafi MM, Rafii YM and Akmar ASN, (2014). Effect of drought stress on growth, proline and antioxidant enzyme activities of upland rice, J, Animal and Plant Sciences 24(5):1487-1493.
Lutts S, Kinet JM and Bouharmont J, (1996). NaClinduced senescence in leaves of rice (Oryza sativa L.) cultivars differing in salinity resistance, Annals of Botany 78(3):389-398.
Noori M, Azar AM, Saidi M, Panahandeh J and Haghi DZ, (2018). Evaluation of water deficiency impacts on antioxidant enzymes activity and lipid peroxidation in some tomato (Solanum lycopersicum L.) lines, Indian Journal of Agricultural Research 52(3):228-235.
Plazas M, Rahma AF, Rodríguez-Burruezo A, Prohens J and Fita A, (2016). Screening for drought tolerance in eggplant relatives and interspecific hybrids, In Proceedings of XVIth Eucarpia Capsicum and Eggplant Working Group Meeting in memoriam Dr, Alain Palloix, 12-14 September 2016, Kecskemét, Hungary (pp, 306- 310).
Plazas M, Nguyen HT, González-Orenga S, Fita A, Vicente O, Prohens J and Boscaiu M, (2019). Comparative analysis of the responses to water stress in eggplant (Solanum melongena) cultivars, Plant Physiology and Biochemistry, 143:72-82.
Prohens J, Whitaker BD, Plazas M, Vilanova S, Hurtado M, Blasco M, Gramazio P and Stommel JR, (2013). Genetic diversity in morphological characters and phenolic acids content resulting from an interspecific cross between eggplant, Solanum melongena, and its wild ancestor (S. incanum). Annals of Applied Biology, 162(2):242-257.
Rotino GL, Sala T and Toppino L, (2014). Eggplant in alien gene transfer in crop plants, Volume 2 (pp. 381-409). Springer, New York, NY.
Sarker BC, Hara M and Uemura M, (2004). Comparison of response of two C3 species to leaf water relation, proline synthesis, gas exchange and water use under periodic water stress, Journal of Plant Biology, 47(1):33-41.
Semida WM, Abdelkhalik A, Mohamed GF, Abd ElMageed TA, Abd El-Mageed SA, Rady MM and Ali EF, (2021). Foliar application of zinc oxide nanoparticles promotes drought stress tolerance in eggplant (Solanum melongena L.). Plants, 10(2):421.
Singh M, Singh P, Singh S, Saini RK and Angadi SV, (2021). A global meta-analysis of yield and water productivity responses of vegetables to deficit irrigation. Scientific reports, 11(1):1-13.
Sseremba G, Tongoona P, Eleblu J, Danquah EY, and Kizito EB, (2018). Heritability of drought resistance in Solanum aethiopicum Shum group and combining ability of genotypes for drought tolerance and recovery, Scientia Horticulturae, 240:213-220.
Wakchaure GC, Minhas PS, Meena KK, Kumar S and Rane J, (2020). Effect of plant growth regulators and deficit irrigation on canopy traits, yield, water productivity and fruit quality of eggplant (Solanum melongena L,) grown in the water scarce environment, Journal of environmental management, 262:110320.
Yekbun A, Kabay T, (2017). Kuraklık stresinin yerli ve ticari domates çeşitlerinde bazı fizyolojik parametreler üzerine etkileri, Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi 22(2):86-96. (in Turkish)
Zhong YP, Li Z, Bai DF, Qi XJ, Chen JY, Wei CG and Fang JB, (2018). In vitro variation of drought tolerance in five Actinidia species, Journal of the American Society for Horticultural Science 143(3):226-234.