Blastocystis'in moleküler epidemiyolojisi

Blastocystis patojenitesi ve sınıflandırması moleküler genetik çalışmalarla yeni aydınlatılmış ve son zamanlarda birçok araştırmacının ilgi odağında bulunan bir parazittir. Blastocystisin genotiplendirilmesinde polimeraz zincir reaksiyonu (PCR), PCR-restriksiyon fragment uzunluk polimorfizimi, rastgele arttırılmış polimorfik DNA, real-time polimeraz zincir reaksiyonu ve DNA dizi analizi gibi çeşitli moleküler yöntemler kullanılabilir. Blastocystis parazitleri ishal, karın ağrısı, şişkinlik, gaz, huzursuzluk, anoreksi, kramp, kusma, dehidratasyon, uykusuzluk, bulantı, iş- tahsızlık, kilo kaybı, yorgunluk, kaşıntı gibi semptomlara neden olduğu gibi asemptomatik olgularda da görülmektedir. Bu derlemede, Blastocystis alt tipleri ve patojenitesi arasındaki ilişkiyi özetlemek amaçlanmıştır

Molecular epidemiology of Blastocystis

Blastocystis pathogenicity and classification was newly illuminated with molecular genetic studies and recently the parasite was found in the focus of many researchers. Several molecular methods such as; polymerase chain reaction (PCR), PCR-restriction fragment length polymorphism, random amplified polymorphic DNA, real-time polymerase chain reaction and DNA sequencing analyses can be used in genotyping of Blastocystis. Blastocystis parasites may cause diarrhea, abdominal pain, bloating, gas, irritability, anorexia, cramps, vomiting, dehydration, insomnia, nausea, loss of appetite, weight loss, fatigue symptoms and also could be asymptomatic cases. In this review, it was aimed to summarize the associations between Blastocystis subtypes and pathogenicity.

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