Savaş ALTINSOY, Başak GÜLEL, Derya ÖZKAN, Ali YALÇINDAĞ, Lütfiye Tuba HANCI, Aslı DÖNMEZ

Diz Protezi Cerrahisi Sırasında Diyabetik Hastalarda İskemi-Reperfüzyon Modelindeki SCUBE-1’in Değeri

Amaç: Diabetes Mellitus (DM), tüm dünyada yüksek mortalite ve morbiditesi olan yaygın bir hastalıktır. Pnömotik turnike kullanılarak diz protezi ameliyatı yapılan ve DM’i olan ve olmayan hastalar arasındaki oksidatif stres seviyesini belirlemek ve signal peptide-CUB-EGF domaincontaining protein 1 (SCUBE-1) seviyelerinin malondialdehit (MDA) ve total antioksidan durumu (TAS) gibi iskemi reperfüzyon (IR) belirteçleriyle korele olup olmadığını araştırmayı amaçladık. Yöntem: Hastalar diyabetik (grup D; n = 15) veya diyabetik olmayan (grup C; n=15) olarak 2 gruba ayrıldı. MDA, TAS ve SCUBE-1 üç dönemde değerlendirildi: spinal anestezi öncesi (T1), turnike indirilmeden 5 dk. önce (T2) ve turnike indirildikten 2 saat sonra (T3). Bulgular: Grupların demografik özellikleri benzerdi. Her 2 grupta da SCUBE-1, MDA ve TAS düzeyleri bakımından tüm zaman dilimlerinde istatistiksel olarak anlamlı fark yoktu. SCUBE-1 seviyeleri T2’de yükseldi ve T3’te neredeyse normal seviyelere döndü. Sonuç: SCUBE-1, MDA ve TAS seviyeleri turnike uygulamasının ardından arttı ve reperfüzyon döneminde azaldı. Bununla birlikte, artışın derecesi, DM olan veya olmayan hastalar arasında farklılık göstermedi. Sonuçlarımız SCUBE-1’in turnike ile ilişkili iskemi-reperfüzyon modelinin bir belirteci olarak kullanılabileceğini göstermektedir.

The Value of SCUBE-1 on Ischemia-Reperfusion ID Model in Diabetic Patients During Knee Replacement Surgery

Objective: Diabetes Mellitus (DM) is a common disease with high mortality and morbidity worldwide.We aimed to assess the oxidative stress levels in patients with and without DM who underwentknee replacement surgery using a pneumatic tourniquet and investigate whether signalpeptide-CUB-EGF domain-containing protein 1 (SCUBE-1) levels are correlated with other ischemia-reperfusion (IR) markers such as malondialdehyde (MDA), and total antioxidant status(TAS).Method: Patients were assigned into either the diabetic (Group D; n=15) or non-diabetic groups(Group C; n=15). MDA, TAS, and SCUBE-1 were assessed at three time points before spinal anesthesia(T1), 5 minutes before (T2) and 2 hours after deflation of the tourniquet (T3).Results: Demographic variables of the groups were similar. There were no statistically significantdifferences in SCUBE-1, MDA and TAS levels of both groups at all time points. SCUBE-1 levels werehigher at T2 and returned to almost normal levels at T3.Conclusion: SCUBE-1, MDA and TAS levels increased following tourniquet application anddecreased during the reperfusion period. The magnitude of increase, however, didn’t differbetween patients with or without DM. Our results suggest that SCUBE-1 may be used as amarker of tourniquet-related ischemia-reperfusion model.

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