Deniz HAZAR, İbrahim BAKTIR

Dianthus orientalis Adams.’ın Üretim Tekniklerinin Belirlenmesi ve Değerlendirilmesi

ÖZ: Bu çalışmanın amacı, Türkiye doğasında bulunan ve sonbaharda son derece dikkat çekici pembe çiçekleriyle peyzaj bitkisi olarak değerlendirilme potansiyeline sahip olan Dianthus orientalis Adams.(Yar karanfili)’ın üretim tekniklerinin belirlenmesi ve değerlendirilmesidir. Bu çalışmada üç farklı üretim yöntemi kullanılmıştır. Bu yöntemler D. orientalis'in tohumla, çelikle ve in vitro ortamda üretimidir. Deneme materyali olarak, bu türün doğadan toplanan tohumları ve çelikleri kullanılmıştır. Tohumların çimlenme oranı ve çeliklerin köklenme oranı belirlenmiştir. D. orientalis'in in vitro tohum çimlenmesi için etkili bir yüzey sterilizasyon protokolü geliştirilmiştir. Tohumlar MS ortamında çimlendirilmiştir. Tohumların MS ortamında çimlenme başarısı belirlenmiştir.

Anahtar Kelimeler:

Dianthus orientalis, yar karanfili, çelik, çimlenme, tohum, in vitro.

Identification and Evaluation of Propagation Techniques of Dianthus orientalis Adams.

ABSTRACT: The aim of the present study was identification and evaluation of propagation techniques of Dianthus orientalis Adams., a Turkish native plant which has good potentials as a landscaping plant with conspicuous pink flowers in autumn. Three different propagation methods were used in this study. These methods are the propagation of D. orientalis by seed, cuttings and in vitro. The seeds and cuttings of this species collected from wild were used as the experimental material. The germination rate of the seeds and the rooting rate of the cuttings were determined. An effective surface sterilization protocol for in vitro seed germination of D. orientalis was developed. The seeds were germinated on MS medium. Germination success of the seeds on MS medium was determined.

Keywords:

Dianthus orientalis, cutting, germination, rooting, seed, in vitro.,

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Al-Absi, K. M. 2010. The effect of pre-sowing seed treatments on breaking the dormancy of mahaleb cherries, Prunus mahaleb L. seeds. Seed Sci. Technol. 38: 332-340.
Anonymous. 1985. SAS User’s Guide: Statistics. Version 5 ed. SAS lnst., Inc. Cary, NC.
Bittrich, V. 1993. Caryophyllaceae. p.206-236. In: K. Kubitzki, J. Rohwer, and V. Bittrich (Eds.) The families and genera of vascular plants, Vol. II, Magnoliid, Hamamelid, and Caryophyllid Families. Springer. Berlin.
Copes, D. L., and N. L. Mandel. 2000. Effect of IBA and NAA on rooting Douglas-fir stem cuttings. New Forests 20: 249-257.
Fincher, G. B. 1989. Molecular and cellular biology associated with endosperm mobilization in germinating cereal grains. Annu. Rev. Plant Physiol Plant Mol. Biol. 40: 305-346.
Galbally, J., and E. Galbally. 1997. Carnations and Pinks for Garden and Greenhouse. Timber Press. Portland, Oregon.
Gubler, F., R. Kalla, J. K. Roberts, and J. V. Jacobsen. 1995. Gibberellin-regulated expression of a myb gene in barley aleurone cells: evidence for MYB transactivation of a high-pI α-amylase gene promoter. Plant Cell 7: 1879-1891.
Hartmann, H.T., D. E. Kester, and Jr. F. T. Davies. 1990. Plant Propagation: Principles and Practices. 5th ed. Prentice Hall, Inc. New Jersey.
Hartmann, H.T., D. E. Kester, Jr. F. T. Davies, and R. L. Geneve. 2002. Hartmann and Kester’s Plant Propagation: Principles and Practices. 7th ed. Prentice Hall, Inc., New Jersey.
Ikinci, A. 2014. Effects of the water presoaking duration and gibberellic acid treatments on seed germination of Argania Spinosa L. under nursery conditions. Fresenius Environmental Bulletin 23 (1): 138-143
Johnson, C. N., D. J. Eakes, A. N. Wright, J. L. Sibley, and L. L. Brune. 2006. Influence of substrates on the propagation of Clematis socialis stem cuttings. SNA Research Conference 51: 357-359.
Khantwal, A., K. S. Negi, and K. Madwal. 2008. Impact of pre sowing treatments on germination of common fodder tree species of lower Siwaliks range of Garhwal Himalayas. Indian J. For. 31 (1): 73-75.
Kumar, A. V., V. Palaniswamy, T. Jayaraj, and R. Arumugam. 1991. Studies on certain seed technological aspects in guava (Psidium guajava L.). South Indian Horticulture 39 (5): 315-316.
Landis, T. D., A. Barthell, and D. Loucks. 1996. Seed treatments to overcome dormancy. Forest Nursery Notes, United States, Department of Agriculture, Forest Services. USA. P: 9-12
Leo, J. 2013. The effect of cold stratification on germination in 28 cultural relict plant species. Nordic Genetic Resource Center, Alnarp. 51pp. From https://stud.epsilon.slu.se/5408/1/leo_j_130410.pdf.
Mayer, A. M., and A. Poljakoff-Mayber. 1989. The Germination of Seeds. 4th ed. Pergamon Press. Oxford. Murashige, T., and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15: 473-497.
Pilon, P. 2006. Dianthus gratianopolitanus ‘Firewitch’. Greenhouse Product News 16: 71-73.
Reeve, H. 1967. Dianthus L. pp. 99-131. In: P. H. Davis (Ed.) Flora of Turkey and the East Aegean Islands. Vol. 2. Edinburgh University Press. Edinburgh.
Riley, J. M. 1987. Gibberellic acid for fruit set and seed germination. CRFG Journal 19: 10-12.
Sakhanokho, H. F. 2009. Sulfuric acid and hot water treatments enhance ex vitro and in vitro germination of Hibiscus seed. African Journal of Biotechnology 8 (22): 6185-6190.
Salisu, A., and S. J. Jiya. 2016. Pre-sowing treatment, formation and transitional changes of leaves from juvenile to adult in Vitex doniana (Linn). Int. J. Plant Biol. Res. 4 (1): 1051.
Sharaf, M. M., A. R. Atawia, K. A. Bakry, and M. Z. EL-Rouby. 2016. Effect of pre-sowing seeds soak in different GA3 and ZnSO4 solutions on germination and growth of Cleopatra mandarin and Rangpur lime rootstocks. Middle East J. Agric. Res. 5 (2): 233-238.
Srivastava, N., V. Sharma, A. K. Dobriyal, B. Kamal, S. Gupta, and V. S. Jadon. 2011. Influence of pre-sowing treatments on in vitro seed germination of ativisha (Aconitum heterophyllum Wall) of Uttarakhand. Biotechnology 10: 215-219.
Steel, R. G. D. and J. H. Torrie. 1980. Principles and Procedures of Statistics: A Biometrical Approach Second Edition, New York: McGraw-Hill Book Co.633 p.
Sujatha, V. N., and K. Manjappa. 2015. Effect of pre-sowing treatments on seed germination of Melia azedarach L. Karnataka J. Agric. Sci. 28 (2): 269-270.